FastQCFastQC Report
Thu 2 Feb 2017
SRR4062282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123655
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30860.1453155055788252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1600.012.47077759
GGTATCA9600.012.4640161
GTATCAA22000.012.3884771
GTATTAG2150.012.3673961
CGTCAAT3400.012.29263819
GCGAATT550.00306534512.09287559
CGAATTA550.003066428212.09230510
CGCGTAA1750.011.94423510
CGAACGA1052.724064E-711.76104316
AAGACGG4050.011.7294725
AGGCCCG4000.011.638844510
GCGTAAC1800.011.61272511
CGGTTCT4850.011.55776312
GCGTTAT1657.2759576E-1211.5107791
TACGCTA2000.011.4018549
ATAGACG752.0749967E-411.3997033
ACGAACG1104.9755545E-711.2264515
GGTTCTA4950.011.13154813
CGCATCG3500.011.12880613
CGAGCCG4100.011.12113815
Produced by FastQC (version 0.11.5)