##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062282_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123655
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21746752650501	32.0	32.0	32.0	32.0	32.0
2	30.675480716029675	32.0	32.0	32.0	32.0	32.0
3	30.705090045228626	32.0	32.0	32.0	32.0	32.0
4	30.712836595398027	32.0	32.0	32.0	32.0	32.0
5	30.598650910811784	32.0	32.0	32.0	32.0	32.0
6	34.26734662645298	36.0	36.0	36.0	32.0	36.0
7	34.19553505630623	36.0	36.0	36.0	32.0	36.0
8	34.138456105158326	36.0	36.0	36.0	32.0	36.0
9	34.30579967085049	36.0	36.0	36.0	32.0	36.0
10	33.93282618881127	36.0	36.0	36.0	32.0	36.0
11	34.30230757820833	36.0	36.0	36.0	32.0	36.0
12	34.08477083142036	36.0	36.0	36.0	32.0	36.0
13	34.188848471149974	36.0	36.0	36.0	32.0	36.0
14	34.07018983780322	36.0	36.0	36.0	32.0	36.0
15	34.00910364442435	36.0	36.0	36.0	32.0	36.0
16	34.01014854107659	36.0	36.0	36.0	32.0	36.0
17	33.94520955616614	36.0	36.0	36.0	32.0	36.0
18	33.951485528487446	36.0	36.0	36.0	32.0	36.0
19	33.97180332963688	36.0	36.0	36.0	32.0	36.0
20	33.9537693269387	36.0	36.0	36.0	32.0	36.0
21	33.92480934991795	36.0	36.0	36.0	32.0	36.0
22	33.90149388671889	36.0	36.0	36.0	32.0	36.0
23	33.87340316576845	36.0	36.0	36.0	32.0	36.0
24	33.83665331704067	36.0	36.0	36.0	32.0	36.0
25	33.46164513539158	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	35.0
5	171.0
6	441.0
7	107.0
8	360.0
9	305.0
10	146.0
11	44.0
12	91.0
13	112.0
14	278.0
15	416.0
16	696.0
17	948.0
18	1353.0
19	1846.0
20	2882.0
21	4269.0
22	6581.0
23	10008.0
24	14207.0
25	20099.0
26	27591.0
27	34838.0
28	46169.0
29	61480.0
30	80159.0
31	108721.0
32	152936.0
33	214359.0
34	452810.0
35	879192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65638633757684	16.992138479540976	11.494112688856607	25.857362494025576
2	16.74891484205996	19.321226527485173	37.239635081797694	26.690223548657173
3	19.40814938850391	22.82520045732712	27.982750404493405	29.78389974967556
4	12.875853806479324	15.439439548805895	35.35747789427681	36.32722875043797
5	15.12906472996952	36.23294630479522	33.20929075752487	15.428698207710392
6	34.97243093126062	34.79856344938006	16.586533704376276	13.642471914983043
7	30.99051518555514	30.0486803234293	19.93470322066983	19.02610127034573
8	28.374124242329962	32.80502671467483	19.002209016520954	19.818640026474256
9	27.04376867539859	14.608557988143922	18.337980280099156	40.00969305635834
10	16.144434469022542	26.699148610314754	31.07426177686574	26.082155143796964
11	37.14354022312069	21.377495348115623	21.950433971535087	19.528530457228598
12	24.635070053158735	23.481835782231194	28.576520946163225	23.306573218446843
13	29.275020109254786	19.4850899187803	24.978429111222802	26.261460860742115
14	23.656077716626235	19.498836004378774	25.005020880261668	31.840065398733326
15	25.26082960955362	26.84747332293174	21.908319812677025	25.983377254837613
16	25.935362082564662	25.95997222165909	23.19722480607729	24.907440889698957
17	24.214159694254253	25.99315032970639	24.81779631849081	24.97489365754855
18	24.831160553750014	25.440660819160062	25.45301205046468	24.27516657662525
19	25.677748998960663	25.161191490164008	25.08322943892305	24.07783007195228
20	25.72667362350317	24.63544951250298	24.616124966653373	25.021751897340472
21	26.438378181437688	24.479943138407357	24.4571777627056	24.624500917449353
22	25.787312310684445	24.378187077000817	24.90551309523922	24.92898751707552
23	24.593235004693987	24.362682999475933	25.180548800488626	25.863533195341454
24	24.618734637799676	25.027937700459773	25.171644235595764	25.181683426144787
25	24.946647562849357	24.567100587253858	25.02238728590685	25.463864563989937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	278.0
1	278.0
2	471.5
3	665.0
4	665.0
5	665.0
6	1306.0
7	1947.0
8	1947.0
9	1947.0
10	2426.5
11	2906.0
12	2906.0
13	2906.0
14	4199.5
15	5493.0
16	5493.0
17	5493.0
18	9232.5
19	12972.0
20	12972.0
21	12972.0
22	20662.5
23	28353.0
24	28353.0
25	28353.0
26	41447.5
27	54542.0
28	54542.0
29	54542.0
30	68573.5
31	82605.0
32	82605.0
33	82605.0
34	104054.5
35	125504.0
36	125504.0
37	125504.0
38	148046.0
39	170588.0
40	170588.0
41	170588.0
42	197706.0
43	224824.0
44	224824.0
45	224824.0
46	249148.5
47	273473.0
48	273473.0
49	273473.0
50	283144.0
51	292815.0
52	292815.0
53	292815.0
54	276696.0
55	260577.0
56	260577.0
57	260577.0
58	238870.0
59	217163.0
60	217163.0
61	217163.0
62	189883.0
63	162603.0
64	162603.0
65	162603.0
66	132724.5
67	102846.0
68	102846.0
69	102846.0
70	77540.5
71	52235.0
72	52235.0
73	52235.0
74	39121.5
75	26008.0
76	26008.0
77	26008.0
78	20381.0
79	14754.0
80	14754.0
81	14754.0
82	10162.0
83	5570.0
84	5570.0
85	5570.0
86	4211.0
87	2852.0
88	2852.0
89	2852.0
90	1838.0
91	824.0
92	824.0
93	824.0
94	551.0
95	278.0
96	278.0
97	278.0
98	629.0
99	980.0
100	980.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.417725572185688E-4
2	4.7088627860928444E-5
3	5.17974906470213E-4
4	0.010500764012987043
5	0.03428052108275591
6	0.06352255898439248
7	0.106608653477142
8	0.11061118684532092
9	0.1200289124175066
10	0.13594486863450045
11	0.1416896812335337
12	0.133590437241454
13	0.12906992896680486
14	0.1183337218145132
15	0.1330724623349838
16	0.12139448262547355
17	0.12605625678370544
18	0.11343650451697662
19	0.09935700478655903
20	0.09422434434971781
21	0.09474231925618803
22	0.10345371541045979
23	0.08433573249892284
24	0.09276459688602905
25	0.09017472235367797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2123655.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.64489104819879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5864930694095	39.26597801306297
2	14.72714762520853	15.506181643596854
3	4.825044294802314	7.620417936078029
4	2.1282160427038908	4.481588067807004
5	1.0539384406191632	2.774223718895218
6	0.6188343036406811	1.9547078695230975
7	0.39875945330222196	1.469485358147435
8	0.2776492385346071	1.1693451129815797
9	0.20986082494024919	0.9943290237838083
>10	1.0153120946681935	9.505149331945324
>50	0.08028574244480856	2.933374103538975
>100	0.06751004964521781	7.200083879753334
>500	0.008166742846929953	2.9525352638674134
>1k	0.002782077233569533	2.1726006770189077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3086	0.1453155055788252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4126588358278534E-4
2	0.0	0.0	0.0	0.0	1.4126588358278534E-4
3	0.0	0.0	0.0	0.0	1.4126588358278534E-4
4	0.0	0.0	0.0	0.0	1.4126588358278534E-4
5	0.0	0.0	0.0	0.0	1.4126588358278534E-4
6	0.0	0.0	0.0	0.0	2.825317671655707E-4
7	0.0	0.0	0.0	0.0	2.825317671655707E-4
8	0.0	0.0	0.0	0.0	2.825317671655707E-4
9	0.0	0.0	0.0	0.0	2.825317671655707E-4
10	0.0	0.0	0.0	0.0	2.825317671655707E-4
11	0.0	0.0	0.0	0.0	2.825317671655707E-4
12	0.0	0.0	0.0	0.0	3.7670902288742755E-4
13	0.0	0.0	0.0	4.7088627860928444E-5	4.2379765074835603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	160	0.0	12.4707775	9
GGTATCA	960	0.0	12.464016	1
GTATCAA	2200	0.0	12.388477	1
GTATTAG	215	0.0	12.367396	1
CGTCAAT	340	0.0	12.292638	19
GCGAATT	55	0.003065345	12.0928755	9
CGAATTA	55	0.0030664282	12.092305	10
CGCGTAA	175	0.0	11.944235	10
CGAACGA	105	2.724064E-7	11.761043	16
AAGACGG	405	0.0	11.729472	5
AGGCCCG	400	0.0	11.6388445	10
GCGTAAC	180	0.0	11.612725	11
CGGTTCT	485	0.0	11.557763	12
GCGTTAT	165	7.2759576E-12	11.510779	1
TACGCTA	200	0.0	11.401854	9
ATAGACG	75	2.0749967E-4	11.399703	3
ACGAACG	110	4.9755545E-7	11.22645	15
GGTTCTA	495	0.0	11.131548	13
CGCATCG	350	0.0	11.128806	13
CGAGCCG	410	0.0	11.121138	15
>>END_MODULE