FastQCFastQC Report
Thu 2 Feb 2017
SRR4062282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123655
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23550.027.4753781
GGTATCA11650.023.236271
GTACGTA654.934566E-420.30725711
TCAACGC35100.018.3016034
ATCAACG35800.018.0052013
CGTCTTA1601.1041266E-917.87461715
CGCAATA4450.017.7982136
AACGCAG36650.017.5275926
CAACGCA36550.017.5153565
CGTCGTA5600.017.28534310
TATCAAC37600.017.2602672
CTAGCGG4600.017.21702429
TCTAGCG4850.016.78314628
CTGTACG1451.250828E-716.6892999
TACGAAT4800.016.50081341
CGAATGC4850.016.330743
TAGCGGC5000.015.83966230
ATACCGT5750.015.6866216
ATACGAA5350.015.62656940
ACCGTCG5600.015.32118
Produced by FastQC (version 0.11.5)