##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123655
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281846627630195	32.0	32.0	32.0	32.0	32.0
2	31.42611299858028	32.0	32.0	32.0	32.0	32.0
3	31.55987672197226	32.0	32.0	32.0	32.0	32.0
4	31.63737000595671	32.0	32.0	32.0	32.0	32.0
5	31.61203020264591	32.0	32.0	32.0	32.0	32.0
6	35.24959138843174	36.0	36.0	36.0	36.0	36.0
7	35.25560131000563	36.0	36.0	36.0	36.0	36.0
8	35.203595216737185	36.0	36.0	36.0	36.0	36.0
9	35.291589735620896	36.0	36.0	36.0	36.0	36.0
10	35.16984726803553	36.0	36.0	36.0	36.0	36.0
11	35.29451676472873	36.0	36.0	36.0	36.0	36.0
12	35.212440344594576	36.0	36.0	36.0	36.0	36.0
13	35.2502261431353	36.0	36.0	36.0	36.0	36.0
14	35.215187966030264	36.0	36.0	36.0	36.0	36.0
15	35.190245590738606	36.0	36.0	36.0	36.0	36.0
16	35.20555645808759	36.0	36.0	36.0	36.0	36.0
17	35.18232999239519	36.0	36.0	36.0	36.0	36.0
18	35.17747374220389	36.0	36.0	36.0	36.0	36.0
19	35.17471387772496	36.0	36.0	36.0	36.0	36.0
20	35.17173787644415	36.0	36.0	36.0	36.0	36.0
21	35.165898886589396	36.0	36.0	36.0	36.0	36.0
22	35.145868796956194	36.0	36.0	36.0	36.0	36.0
23	35.116545766614635	36.0	36.0	36.0	36.0	36.0
24	35.09743531788355	36.0	36.0	36.0	36.0	36.0
25	35.088161683512624	36.0	36.0	36.0	36.0	36.0
26	35.04234350683138	36.0	36.0	36.0	36.0	36.0
27	35.02814958173526	36.0	36.0	36.0	36.0	36.0
28	35.00906126465928	36.0	36.0	36.0	36.0	36.0
29	34.99541215498751	36.0	36.0	36.0	36.0	36.0
30	34.97615761505518	36.0	36.0	36.0	36.0	36.0
31	34.97310956817374	36.0	36.0	36.0	36.0	36.0
32	34.94812104602678	36.0	36.0	36.0	36.0	36.0
33	34.93084281580577	36.0	36.0	36.0	36.0	36.0
34	34.921489130767476	36.0	36.0	36.0	36.0	36.0
35	34.91232191669551	36.0	36.0	36.0	36.0	36.0
36	34.88971089936925	36.0	36.0	36.0	32.0	36.0
37	34.88145485024639	36.0	36.0	36.0	32.0	36.0
38	34.86025272466573	36.0	36.0	36.0	32.0	36.0
39	34.84410980126245	36.0	36.0	36.0	32.0	36.0
40	34.83264324949203	36.0	36.0	36.0	32.0	36.0
41	34.805792372113174	36.0	36.0	36.0	32.0	36.0
42	34.77516781209754	36.0	36.0	36.0	32.0	36.0
43	34.77822574759083	36.0	36.0	36.0	32.0	36.0
44	34.73290765213747	36.0	36.0	36.0	32.0	36.0
45	34.71806060777292	36.0	36.0	36.0	32.0	36.0
46	34.70332798877407	36.0	36.0	36.0	32.0	36.0
47	34.67739722318362	36.0	36.0	36.0	32.0	36.0
48	34.65530700608149	36.0	36.0	36.0	32.0	36.0
49	34.64065726306768	36.0	36.0	36.0	32.0	36.0
50	34.22110041414448	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	19.0
21	62.0
22	148.0
23	407.0
24	1043.0
25	2509.0
26	5033.0
27	9451.0
28	16329.0
29	26107.0
30	39230.0
31	57820.0
32	89048.0
33	152785.0
34	349842.0
35	1373812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27415438400474	17.56074836350242	11.924752906175708	26.240344346317134
2	16.223513966091097	19.856779049639	36.889872347158466	27.02983463711143
3	18.71195226327527	23.379957507082153	28.284913507323246	29.62317672231933
4	12.541820587618988	15.88374759553694	35.6047945640888	35.96963725275527
5	14.749994702529365	36.58937068403295	33.10561272899788	15.555021884439798
6	35.103084263845616	34.931869690452906	16.556251474465437	13.408794571236045
7	31.010545498209456	30.07206914494115	19.96538985852222	18.951995498327175
8	28.43442084519378	32.95186835903195	18.869307867803386	19.74440292797088
9	27.258900339273563	14.374227452199156	18.22555923631663	40.14131297221065
10	16.08196634853545	26.76121134724522	31.216696520898907	25.940125783320433
11	37.44746674954265	21.25269876698428	21.95606160134297	19.3437728821301
12	24.82173422707549	23.44283793742392	28.466394023511356	23.269033811989235
13	29.461753439235654	19.374757199262593	25.0828877571922	26.08060160430955
14	23.71510438371581	19.356204279885386	24.98560265203152	31.943088684367282
15	25.2869227817136	26.822953822537087	21.976686420346052	25.913436975403254
16	25.982685430003606	25.902210586044276	23.20336026030716	24.91174372364496
17	24.340959336615413	25.929588374759554	24.795694215868398	24.93375807275664
18	24.95285246695199	25.31322207535788	25.453357963848145	24.28056749384198
19	25.790877421651548	25.07927374233279	25.132060119021272	23.99778871699439
20	25.832824381829937	24.459196843919916	24.727697211448042	24.980281562802105
21	26.645759315896417	24.358523394807538	24.506758395313742	24.48895889398231
22	25.88508962143098	24.2956600766132	24.949862383485076	24.86938791847075
23	24.623396217175046	24.304464339950396	25.246897913075173	25.825241529799385
24	24.731286087904035	24.922703253008756	25.20462565583335	25.14138500325386
25	25.12875232323955	24.378723245957083	25.14273767205629	25.349786758747083
26	24.91243816048091	25.21182948915118	25.290985957143437	24.58474639322447
27	25.363019371863594	24.88280773593728	24.756327205039256	24.997845687159874
28	24.9549941962173	24.701746785551997	25.31866651911952	25.024592499111186
29	24.790619528866863	25.041414623717888	25.200433593922906	24.96753225349234
30	24.895991621880867	24.939690444696737	25.406439078049015	24.757878855373377
31	25.345938070657724	24.79627018815754	24.632542335210296	25.22524940597444
32	25.254658537182596	24.785508573064586	24.546530748323153	25.413302141429668
33	24.81481150115815	24.637380882981024	24.991582876061795	25.55622473979903
34	25.369342609239425	24.721868793080553	25.253362390388567	24.655426207291452
35	25.851729734998578	24.421596977654968	25.210945876649106	24.51572741069734
36	24.832893917126682	25.010983443136368	24.838262030813915	25.31786060892303
37	25.64224030559125	24.86634856219636	24.866960724159348	24.62445040805304
38	25.01559810797893	24.629283005007878	25.152720192309957	25.202398694703238
39	25.54717578186804	24.691801592742998	24.676120909837486	25.08490171555147
40	25.675463140417413	24.721211647471762	24.947425522236674	24.65589968987415
41	24.743435074028763	25.313448550927326	25.181928712763124	24.76118766228079
42	25.818892810017996	25.030161927876964	24.92208757272062	24.228857689384416
43	25.437047459746992	24.203792861825004	25.13642134370079	25.22273833472721
44	24.867393241199263	24.988227382918968	24.929599749855434	25.214779626026335
45	25.196975718728282	25.101007151965614	24.80081107094893	24.901206058357175
46	24.975124846664045	24.97747933349187	24.898368576077	25.149027243767087
47	25.05177455583129	24.8677500317852	25.188427362581994	24.892048049801517
48	25.78370533338733	25.2694288757684	24.352857410600848	24.59400838024342
49	25.08356227214035	25.291514367813384	24.58275372651474	25.042169633531525
50	25.28082824259793	25.27998063691916	24.50908327207813	24.930107848404784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	114.0
1	87.5
2	61.0
3	141.0
4	221.0
5	252.0
6	283.0
7	221.5
8	160.0
9	232.5
10	305.0
11	527.5
12	750.0
13	1316.0
14	1882.0
15	2564.5
16	3247.0
17	3293.5
18	3340.0
19	3378.0
20	3416.0
21	4261.5
22	5107.0
23	6294.5
24	7482.0
25	9744.0
26	12006.0
27	14915.0
28	17824.0
29	21438.5
30	25053.0
31	28550.5
32	32048.0
33	36563.5
34	41079.0
35	47378.5
36	53678.0
37	63776.5
38	73875.0
39	82173.5
40	90472.0
41	99041.5
42	107611.0
43	113590.0
44	119569.0
45	130461.0
46	141353.0
47	149383.5
48	157414.0
49	163810.0
50	170206.0
51	166746.0
52	163286.0
53	162079.0
54	160872.0
55	161884.5
56	162897.0
57	156617.5
58	150338.0
59	136180.5
60	122023.0
61	107273.0
62	92523.0
63	78822.5
64	65122.0
65	54818.0
66	44514.0
67	38026.0
68	31538.0
69	27655.5
70	23773.0
71	18783.5
72	13794.0
73	11971.5
74	10149.0
75	7428.0
76	4707.0
77	4193.5
78	3680.0
79	3112.0
80	2544.0
81	1997.0
82	1450.0
83	1231.0
84	1012.0
85	780.5
86	549.0
87	366.0
88	183.0
89	117.5
90	52.0
91	37.5
92	23.0
93	27.5
94	32.0
95	26.0
96	20.0
97	18.0
98	16.0
99	9.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03183191243398763
2	0.003908356112457061
3	3.296203950264991E-4
4	0.0
5	0.0
6	1.8835451144371377E-4
7	0.0
8	0.0
9	0.0
10	1.4126588358278534E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.708862786092844E-4
17	0.0
18	9.417725572185689E-5
19	4.7088627860928444E-5
20	3.7670902288742755E-4
21	0.0
22	0.0
23	1.8835451144371377E-4
24	9.888611850794973E-4
25	1.8835451144371377E-4
26	6.121521621920698E-4
27	3.7670902288742755E-4
28	0.0018835451144371376
29	4.2379765074835603E-4
30	0.0013184815801059965
31	4.2379765074835603E-4
32	0.001271392952245068
33	3.7670902288742755E-4
34	8.005066736357836E-4
35	2.825317671655707E-4
36	1.8835451144371377E-4
37	0.0016010133472715673
38	0.0
39	0.0010830384408013543
40	4.7088627860928444E-5
41	9.888611850794973E-4
42	0.005509369459728628
43	0.004096710623900775
44	0.003908356112457061
45	0.0020248109980199234
46	0.0023544313930464224
47	0.0011772156965232112
48	0.004190887879622632
49	0.004190887879622632
50	0.0012243043243841396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2123655.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.27035128293355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75456724788502	39.2893170601558
2	15.269190385630216	16.267902712970265
3	5.095452516252851	8.14309636458891
4	2.1416739537600082	4.563508954012193
5	1.083717624135375	2.8865009264598784
6	0.6527864196015009	2.0864497130940243
7	0.40324068561399695	1.5036541081960009
8	0.28693481224847983	1.2228094595023282
9	0.19222074987955864	0.9215700182957696
>10	0.9743195696111993	9.20045934899016
>50	0.0745299644676981	2.7719942353199736
>100	0.06122497859841355	6.510401844364103
>500	0.007561340761790632	2.72668709738808
>1k	0.0025797515540226658	1.9056481566625576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7088627860928444E-5
2	0.0	0.0	0.0	0.0	4.7088627860928444E-5
3	0.0	0.0	0.0	0.0	4.7088627860928444E-5
4	0.0	0.0	0.0	0.0	4.7088627860928444E-5
5	0.0	0.0	0.0	0.0	4.7088627860928444E-5
6	0.0	0.0	0.0	0.0	4.7088627860928444E-5
7	0.0	0.0	0.0	0.0	4.7088627860928444E-5
8	0.0	0.0	0.0	0.0	4.7088627860928444E-5
9	0.0	0.0	0.0	0.0	4.7088627860928444E-5
10	0.0	0.0	0.0	0.0	4.7088627860928444E-5
11	0.0	0.0	0.0	0.0	4.7088627860928444E-5
12	0.0	0.0	0.0	0.0	2.3544313930464223E-4
13	0.0	0.0	0.0	4.7088627860928444E-5	2.825317671655707E-4
14	0.0	0.0	0.0	9.417725572185689E-5	2.825317671655707E-4
15	0.0	0.0	0.0	2.3544313930464223E-4	3.7670902288742755E-4
16	0.0	0.0	0.0	3.296203950264991E-4	3.7670902288742755E-4
17	0.0	0.0	0.0	3.296203950264991E-4	3.7670902288742755E-4
18	0.0	0.0	0.0	4.2379765074835603E-4	4.2379765074835603E-4
19	0.0	0.0	0.0	5.17974906470213E-4	4.7088627860928446E-4
20	0.0	0.0	0.0	6.592407900529982E-4	6.121521621920698E-4
21	0.0	0.0	0.0	7.534180457748551E-4	6.592407900529982E-4
22	0.0	0.0	0.0	8.475953014967121E-4	7.534180457748551E-4
23	0.0	0.0	0.0	0.0012243043243841396	7.534180457748551E-4
24	0.0	0.0	0.0	0.0017422792308543526	7.534180457748551E-4
25	0.0	0.0	0.0	0.0025898745323510645	7.534180457748551E-4
26	0.0	0.0	0.0	0.0032962039502649914	9.417725572185689E-4
27	0.0	0.0	0.0	0.003814178856735204	9.888611850794975E-4
28	0.0	0.0	0.0	0.004661774158231916	9.888611850794975E-4
29	0.0	0.0	0.0	0.0061686102497816265	9.888611850794975E-4
30	0.0	0.0	0.0	0.00819342124780155	9.888611850794975E-4
31	0.0	0.0	0.0	0.011583802453788398	9.888611850794975E-4
32	0.0	0.0	0.0	0.01662228563490774	9.888611850794975E-4
33	0.0	0.0	0.0	0.02123697116527873	9.888611850794975E-4
34	0.0	0.0	0.0	0.02571039081206693	0.0010830384408013543
35	0.0	0.0	0.0	0.030654696737464418	0.0010830384408013543
36	0.0	0.0	0.0	0.037859256800186473	0.0010830384408013543
37	0.0	0.0	0.0	0.0491605274868093	0.0010830384408013543
38	0.0	0.0	0.0	0.06427597703016732	0.0010830384408013543
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2355	0.0	27.475378	1
GGTATCA	1165	0.0	23.23627	1
GTACGTA	65	4.934566E-4	20.307257	11
TCAACGC	3510	0.0	18.301603	4
ATCAACG	3580	0.0	18.005201	3
CGTCTTA	160	1.1041266E-9	17.874617	15
CGCAATA	445	0.0	17.79821	36
AACGCAG	3665	0.0	17.527592	6
CAACGCA	3655	0.0	17.515356	5
CGTCGTA	560	0.0	17.285343	10
TATCAAC	3760	0.0	17.260267	2
CTAGCGG	460	0.0	17.217024	29
TCTAGCG	485	0.0	16.783146	28
CTGTACG	145	1.250828E-7	16.689299	9
TACGAAT	480	0.0	16.500813	41
CGAATGC	485	0.0	16.3307	43
TAGCGGC	500	0.0	15.839662	30
ATACCGT	575	0.0	15.686621	6
ATACGAA	535	0.0	15.626569	40
ACCGTCG	560	0.0	15.3211	8
>>END_MODULE