FastQCFastQC Report
Thu 2 Feb 2017
SRR4062281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82723
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC20772.510789018773497No Hit
CCCATGTACTCTGCGTTGATACCAC15121.8277867098630367No Hit
GAGTACATGGGAAGCAGTGGTATCA6930.8377355753538919No Hit
GTATCAACGCAGAGTACATGGGAAG5950.7192679182331395No Hit
CATGTACTCTGCGTTGATACCACTG5360.6479455532318702No Hit
GCGTTGATACCACTGCTTCCCATGT5230.6322304558587092No Hit
TATCAACGCAGAGTACATGGGAAGC5100.6165153584855482No Hit
GTACATGGAAGCAGTGGTATCAACG5070.6128887975532803No Hit
GTATCAACGCAGAGTACTTTTTTTT4840.5850851637392261No Hit
TATCAACGCAGAGTACTTTTTTTTT4180.5053008232293316No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4170.5040919695852423No Hit
GCTTCCCATGTACTCTGCGTTGATA4160.502883115941153No Hit
TCCATGTACTCTGCGTTGATACCAC4100.4956299940766172No Hit
ACGCAGAGTACATGGGAAGCAGTGG3710.448484701957134No Hit
GGTATCAACGCAGAGTACATGGGAA3580.432769604583973No Hit
ACTCTGCGTTGATACCACTGCTTCC2790.33727016670091753No Hit
AAAAAGTACTCTGCGTTGATACCAC2600.31430194746322065No Hit
GGTATCAACGCAGAGTACTTTTTTT2530.3058399719545955No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2430.29375143551370236No Hit
CAGTGGTATCAACGCAGAGTACATG2310.27924519178463064No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2050.2478149970383086No Hit
GTACTCTGCGTTGATACCACTGCTT1990.24056187517377273No Hit
GTGGTATCAACGCAGAGTACATGGG1720.20792282678336135No Hit
GAGTACATGGAAGCAGTGGTATCAA1710.20671397313927203No Hit
GGGAAGCAGTGGTATCAACGCAGAG1540.18616346118975377No Hit
GCAGTGGTATCAACGCAGAGTACAT1510.1825369002574858No Hit
CTGCTTCCCATGTACTCTGCGTTGA1480.17891033932521788No Hit
GCAGAGTACATGGGAAGCAGTGGTA1390.1680306565284141No Hit
AAGCAGTGGTATCAACGCAGAGTAC1340.16198638830796755No Hit
AAAGTACTCTGCGTTGATACCACTG1220.14748014457889583No Hit
GCTTCCATGTACTCTGCGTTGATAC1210.14627129093480654No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1180.1426447300025386No Hit
ATACCACTGCTTCCCATGTACTCTG1120.13539160813800272No Hit
TACCACTGCTTCCCATGTACTCTGC1020.12330307169710963No Hit
CCACTGCTTCCCATGTACTCTGCGT960.11604994983257376No Hit
GTTGATACCACTGCTTCCCATGTAC960.11604994983257376No Hit
ATCAACGCAGAGTACATGGGAAGCA950.11484109618848447No Hit
CCCCATGTACTCTGCGTTGATACCA910.11000568161212722No Hit
CCATGTACTCTGCGTTGATACCACT910.11000568161212722No Hit
CATGGAAGCAGTGGTATCAACGCAG910.11000568161212722No Hit
CATGGGAAGCAGTGGTATCAACGCA910.11000568161212722No Hit
GCGTTGATACCACTGCTTCCATGTA850.10275255974759136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA504.4495937E-617.1032712
AAAAAGT504.4495937E-617.1032711
CCATGTA4400.010.1496692
AAAGTAC856.4419024E-410.0607483
TGCGTTG5000.09.87590812
GGGAAGC4550.09.8150647
ATGTACT4600.09.7083784
TGTACTC4650.09.6039885
CATGTAC4650.09.6039883
TCTGCGT5550.09.58741910
CTCTGCG5800.09.5018189
CCCATGT4100.09.5018171
AGTACTC900.00108828299.5018175
GAAGCAG4750.09.4017979
GGAAGCA4750.09.4017978
GTACTCT5700.09.3351186
CTGCGTT5400.09.32585711
AGCAGTG5050.09.21958511
AGTGGTA5150.09.219480514
CAGTGGT5150.09.219480513
Produced by FastQC (version 0.11.5)