##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062281_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82723
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60813800273201	32.0	32.0	32.0	32.0	32.0
2	30.26136624638855	32.0	32.0	32.0	21.0	32.0
3	30.075069811297947	32.0	32.0	32.0	21.0	32.0
4	30.17741136080655	32.0	32.0	32.0	21.0	32.0
5	30.057976620770525	32.0	32.0	32.0	21.0	32.0
6	33.575208829467016	36.0	36.0	36.0	21.0	36.0
7	33.32633004122191	36.0	36.0	36.0	21.0	36.0
8	33.17310784183359	36.0	36.0	36.0	21.0	36.0
9	33.45984792621157	36.0	36.0	36.0	21.0	36.0
10	33.02225499558769	36.0	36.0	36.0	14.0	36.0
11	33.43985348693834	36.0	36.0	36.0	21.0	36.0
12	33.20722169166979	36.0	36.0	36.0	21.0	36.0
13	33.19944876273829	36.0	36.0	36.0	21.0	36.0
14	33.13200681793455	36.0	36.0	36.0	21.0	36.0
15	33.12644609117174	36.0	36.0	36.0	21.0	36.0
16	33.150284685033185	36.0	36.0	36.0	21.0	36.0
17	32.847926211573565	36.0	36.0	36.0	14.0	36.0
18	33.00236935314241	36.0	36.0	36.0	14.0	36.0
19	33.01848337221813	36.0	36.0	36.0	14.0	36.0
20	32.84740640450661	36.0	36.0	36.0	14.0	36.0
21	32.782986593813085	36.0	36.0	36.0	14.0	36.0
22	32.75572694413887	36.0	36.0	36.0	14.0	36.0
23	32.58501263252058	36.0	36.0	36.0	14.0	36.0
24	32.49932908622753	36.0	36.0	36.0	14.0	36.0
25	32.07007724574786	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	6.0
6	13.0
7	3.0
8	21.0
9	8.0
10	7.0
11	0.0
12	3.0
13	4.0
14	73.0
15	104.0
16	132.0
17	155.0
18	184.0
19	272.0
20	346.0
21	440.0
22	606.0
23	798.0
24	935.0
25	1292.0
26	1582.0
27	1966.0
28	2489.0
29	3327.0
30	4205.0
31	5881.0
32	7719.0
33	9953.0
34	17718.0
35	22480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.184545828195645	16.92415560552211	11.271487633277726	24.619810933004523
2	13.906652321603424	21.70738488691174	37.46358328397181	26.92237950751303
3	19.648707131027646	28.4406996844892	27.376908477690602	24.53368470679255
4	12.203201315344069	17.78132404855167	33.98254267614488	36.03293195995938
5	11.824381514352735	39.60605547628836	34.440521390051025	14.129041619307877
6	32.723687871200305	33.05875095257104	19.0163418853044	15.201219290924268
7	27.00759959339755	31.36284428094293	22.77578779224551	18.85376833341401
8	28.5218169937804	32.97354855884417	20.85017303550253	17.654461411872898
9	28.374862343131678	14.085171783669962	18.354652499606694	39.18531337359166
10	15.550714112805617	29.94069232631324	32.44977003146938	22.058823529411764
11	32.85479107151503	23.242386094029925	21.63971335883407	22.263109475620976
12	24.343036978756885	25.921442837257157	30.0187617260788	19.71675845790716
13	30.913822319051075	20.21786492374728	23.810215444202374	25.058097312999273
14	23.11807135595682	21.59679527520937	26.78026818995982	28.504865178873988
15	28.89251997095134	24.302832244008716	24.101912369886225	22.702735415153715
16	21.81232828631253	26.181571717318423	29.43369279740508	22.57240719896397
17	24.61540323654365	25.78946731381401	28.008085307253776	21.587044142388553
18	25.561216463156367	23.373228613265887	29.15179165708615	21.913763266491596
19	23.49595856928513	27.56642950486424	28.86113934465902	20.076472581191616
20	24.17727338721386	24.939505396118665	29.270919034022164	21.61230218264531
21	22.697300763432665	26.052290902931535	28.722490411721292	22.527917921914508
22	23.226235856477278	26.4197979064561	24.49930416893568	25.854662068130935
23	20.795121833184115	27.410651632104923	26.856534468991217	24.937692065719748
24	22.17059891107078	29.943133696309737	25.93829401088929	21.947973381730186
25	23.41197822141561	24.579552329098608	25.040532365396253	26.967937084089534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	98.0
2	122.5
3	147.0
4	147.0
5	147.0
6	338.0
7	529.0
8	529.0
9	529.0
10	478.5
11	428.0
12	428.0
13	428.0
14	385.5
15	343.0
16	343.0
17	343.0
18	572.5
19	802.0
20	802.0
21	802.0
22	1179.5
23	1557.0
24	1557.0
25	1557.0
26	2216.5
27	2876.0
28	2876.0
29	2876.0
30	3415.0
31	3954.0
32	3954.0
33	3954.0
34	4298.5
35	4643.0
36	4643.0
37	4643.0
38	5339.5
39	6036.0
40	6036.0
41	6036.0
42	7159.5
43	8283.0
44	8283.0
45	8283.0
46	12969.5
47	17656.0
48	17656.0
49	17656.0
50	15178.0
51	12700.0
52	12700.0
53	12700.0
54	10410.0
55	8120.0
56	8120.0
57	8120.0
58	6997.5
59	5875.0
60	5875.0
61	5875.0
62	4905.5
63	3936.0
64	3936.0
65	3936.0
66	3190.0
67	2444.0
68	2444.0
69	2444.0
70	1833.0
71	1222.0
72	1222.0
73	1222.0
74	903.0
75	584.0
76	584.0
77	584.0
78	424.5
79	265.0
80	265.0
81	265.0
82	189.0
83	113.0
84	113.0
85	113.0
86	82.0
87	51.0
88	51.0
89	51.0
90	36.5
91	22.0
92	22.0
93	22.0
94	16.0
95	10.0
96	10.0
97	10.0
98	19.5
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012088536440893103
2	0.0
3	0.0
4	0.00846197550862517
5	0.025385926525875513
6	0.06286038949264412
7	0.10517026703576998
8	0.09791714517123412
9	0.1087968279680379
10	0.12451192534119894
11	0.13418275449391343
12	0.1305561935616455
13	0.12451192534119894
14	0.11484109618848447
15	0.12451192534119894
16	0.12088536440893101
17	0.12572077898528825
18	0.1087968279680379
19	0.0954994378830555
20	0.08582860873034101
21	0.0846197550862517
22	0.10637912067985929
23	0.08341090144216239
24	0.08824631601851964
25	0.08824631601851964
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	82723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.89395935834049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.38936713650553	53.91970794095958
2	10.871251537224481	13.89214607787435
3	2.5957809100368934	4.9756415990716
4	0.944092328067354	2.412871873602263
5	0.3462302525778072	1.1061010843417187
6	0.1816289849588497	0.6962996989954426
7	0.12108598997256645	0.5415664325520109
8	0.08324661810613944	0.42551648271943715
9	0.05297512061299783	0.30463111831050615
>10	0.3046069435247375	3.7027187118455567
>50	0.04540724623971242	2.1372532427499
>100	0.04919118342635512	7.480386349624651
>500	0.011351811559928105	4.066583658716439
>1k	0.0037839371866427015	4.338575728636534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2077	2.510789018773497	No Hit
CCCATGTACTCTGCGTTGATACCAC	1512	1.8277867098630367	No Hit
GAGTACATGGGAAGCAGTGGTATCA	693	0.8377355753538919	No Hit
GTATCAACGCAGAGTACATGGGAAG	595	0.7192679182331395	No Hit
CATGTACTCTGCGTTGATACCACTG	536	0.6479455532318702	No Hit
GCGTTGATACCACTGCTTCCCATGT	523	0.6322304558587092	No Hit
TATCAACGCAGAGTACATGGGAAGC	510	0.6165153584855482	No Hit
GTACATGGAAGCAGTGGTATCAACG	507	0.6128887975532803	No Hit
GTATCAACGCAGAGTACTTTTTTTT	484	0.5850851637392261	No Hit
TATCAACGCAGAGTACTTTTTTTTT	418	0.5053008232293316	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	417	0.5040919695852423	No Hit
GCTTCCCATGTACTCTGCGTTGATA	416	0.502883115941153	No Hit
TCCATGTACTCTGCGTTGATACCAC	410	0.4956299940766172	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	371	0.448484701957134	No Hit
GGTATCAACGCAGAGTACATGGGAA	358	0.432769604583973	No Hit
ACTCTGCGTTGATACCACTGCTTCC	279	0.33727016670091753	No Hit
AAAAAGTACTCTGCGTTGATACCAC	260	0.31430194746322065	No Hit
GGTATCAACGCAGAGTACTTTTTTT	253	0.3058399719545955	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	243	0.29375143551370236	No Hit
CAGTGGTATCAACGCAGAGTACATG	231	0.27924519178463064	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	205	0.2478149970383086	No Hit
GTACTCTGCGTTGATACCACTGCTT	199	0.24056187517377273	No Hit
GTGGTATCAACGCAGAGTACATGGG	172	0.20792282678336135	No Hit
GAGTACATGGAAGCAGTGGTATCAA	171	0.20671397313927203	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	154	0.18616346118975377	No Hit
GCAGTGGTATCAACGCAGAGTACAT	151	0.1825369002574858	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	148	0.17891033932521788	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	139	0.1680306565284141	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	134	0.16198638830796755	No Hit
AAAGTACTCTGCGTTGATACCACTG	122	0.14748014457889583	No Hit
GCTTCCATGTACTCTGCGTTGATAC	121	0.14627129093480654	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	118	0.1426447300025386	No Hit
ATACCACTGCTTCCCATGTACTCTG	112	0.13539160813800272	No Hit
TACCACTGCTTCCCATGTACTCTGC	102	0.12330307169710963	No Hit
CCACTGCTTCCCATGTACTCTGCGT	96	0.11604994983257376	No Hit
GTTGATACCACTGCTTCCCATGTAC	96	0.11604994983257376	No Hit
ATCAACGCAGAGTACATGGGAAGCA	95	0.11484109618848447	No Hit
CCCCATGTACTCTGCGTTGATACCA	91	0.11000568161212722	No Hit
CCATGTACTCTGCGTTGATACCACT	91	0.11000568161212722	No Hit
CATGGAAGCAGTGGTATCAACGCAG	91	0.11000568161212722	No Hit
CATGGGAAGCAGTGGTATCAACGCA	91	0.11000568161212722	No Hit
GCGTTGATACCACTGCTTCCATGTA	85	0.10275255974759136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.00120885364408931
13	0.0	0.0	0.0	0.0	0.00120885364408931
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	50	4.4495937E-6	17.103271	2
AAAAAGT	50	4.4495937E-6	17.103271	1
CCATGTA	440	0.0	10.149669	2
AAAGTAC	85	6.4419024E-4	10.060748	3
TGCGTTG	500	0.0	9.875908	12
GGGAAGC	455	0.0	9.815064	7
ATGTACT	460	0.0	9.708378	4
TGTACTC	465	0.0	9.603988	5
CATGTAC	465	0.0	9.603988	3
TCTGCGT	555	0.0	9.587419	10
CTCTGCG	580	0.0	9.501818	9
CCCATGT	410	0.0	9.501817	1
AGTACTC	90	0.0010882829	9.501817	5
GAAGCAG	475	0.0	9.401797	9
GGAAGCA	475	0.0	9.401797	8
GTACTCT	570	0.0	9.335118	6
CTGCGTT	540	0.0	9.325857	11
AGCAGTG	505	0.0	9.219585	11
AGTGGTA	515	0.0	9.2194805	14
CAGTGGT	515	0.0	9.2194805	13
>>END_MODULE