FastQCFastQC Report
Thu 2 Feb 2017
SRR4062281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82723
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7880.9525766715423764No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5390.6515721141641382No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3230.3904597270408472No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2460.2973779964459703No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2430.29375143551370236No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2170.2623212407673803No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1740.21034053407153996No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1720.20792282678336135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.2042962658510934No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1620.19583429034246824No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1610.19462543669837892No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1440.17407492474886066No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1300.15715097373161033No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1170.1414358763584493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.12934733991755618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.12330307169710963No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC970.11725880347666308No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG860.10396141339168068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGAG250.002340970635.1975825
GGGCCTG250.002340970635.1975825
TATAAGA353.1821715E-431.4454252
GGAATAT300.00571643529.3313212
TATGGCG300.00571643529.3313216
AGAGTGA300.00571643529.331328
AAAGTAC450.001387798524.4427663
ATATACA500.002552382922.0117972
AAGTACT602.8428884E-421.998494
ATATGGC500.00256137521.9984915
TATACAC500.00256137521.998493
CAGAGGA500.00256137521.998494
GCTGGAC654.8812066E-420.30633
GAATATG550.004450763619.99862713
TGGACCC708.039987E-418.8558485
AAATGAG600.007332589518.34316443
CTGAAAA750.001277720917.59879129
GCTTCCA1751.0197582E-613.82762127
TGCTTCC8050.013.66365626
ACCACTG8300.013.25210221
Produced by FastQC (version 0.11.5)