##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82723
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.66638057130423	32.0	32.0	32.0	32.0	32.0
2	30.98996651475406	32.0	32.0	32.0	32.0	32.0
3	31.30724224218174	32.0	32.0	32.0	32.0	32.0
4	31.47899616793395	32.0	32.0	32.0	32.0	32.0
5	31.430085949494096	32.0	32.0	32.0	32.0	32.0
6	34.97096333546897	36.0	36.0	36.0	36.0	36.0
7	35.01072253182307	36.0	36.0	36.0	36.0	36.0
8	34.913675761275584	36.0	36.0	36.0	36.0	36.0
9	35.034718276658246	36.0	36.0	36.0	36.0	36.0
10	34.846064576961666	36.0	36.0	36.0	32.0	36.0
11	35.042974747047374	36.0	36.0	36.0	36.0	36.0
12	34.94682252819651	36.0	36.0	36.0	36.0	36.0
13	34.96598285845533	36.0	36.0	36.0	36.0	36.0
14	34.910762423993326	36.0	36.0	36.0	32.0	36.0
15	34.81433216880432	36.0	36.0	36.0	32.0	36.0
16	34.84176105798871	36.0	36.0	36.0	32.0	36.0
17	34.81251888833819	36.0	36.0	36.0	32.0	36.0
18	34.767682506678916	36.0	36.0	36.0	32.0	36.0
19	34.72953108567146	36.0	36.0	36.0	32.0	36.0
20	34.71854260604668	36.0	36.0	36.0	32.0	36.0
21	34.68829708787157	36.0	36.0	36.0	32.0	36.0
22	34.553618703383584	36.0	36.0	36.0	32.0	36.0
23	34.42943316852629	36.0	36.0	36.0	32.0	36.0
24	34.36720138292857	36.0	36.0	36.0	32.0	36.0
25	34.37019933996591	36.0	36.0	36.0	32.0	36.0
26	34.21840358787762	36.0	36.0	36.0	32.0	36.0
27	34.137676341525335	36.0	36.0	36.0	32.0	36.0
28	34.059499776362074	36.0	36.0	36.0	32.0	36.0
29	33.903593921883875	36.0	36.0	36.0	32.0	36.0
30	33.94869625134485	36.0	36.0	36.0	32.0	36.0
31	33.764503221594964	36.0	36.0	36.0	32.0	36.0
32	33.77408943099259	36.0	36.0	36.0	32.0	36.0
33	33.62503777667638	36.0	36.0	36.0	27.0	36.0
34	33.57358896558394	36.0	36.0	36.0	27.0	36.0
35	33.440627153270555	36.0	36.0	36.0	21.0	36.0
36	33.2614992202894	36.0	36.0	36.0	21.0	36.0
37	33.22601936583538	36.0	36.0	36.0	21.0	36.0
38	33.11220579524437	36.0	36.0	36.0	14.0	36.0
39	32.87591117343423	36.0	36.0	36.0	14.0	36.0
40	32.591044812204586	36.0	36.0	36.0	14.0	36.0
41	32.48864282001378	36.0	36.0	36.0	14.0	36.0
42	32.390834471670516	36.0	36.0	36.0	14.0	36.0
43	32.25741329497238	36.0	36.0	36.0	14.0	36.0
44	32.38989156582812	36.0	36.0	36.0	14.0	36.0
45	32.004025482634816	36.0	32.0	36.0	14.0	36.0
46	31.918329847805325	36.0	32.0	36.0	14.0	36.0
47	31.983462882148856	36.0	36.0	36.0	14.0	36.0
48	31.816532282436565	36.0	32.0	36.0	14.0	36.0
49	31.97807139489622	36.0	32.0	36.0	14.0	36.0
50	31.39270819481885	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	20.0
22	49.0
23	95.0
24	208.0
25	407.0
26	790.0
27	1499.0
28	2151.0
29	3274.0
30	4370.0
31	5675.0
32	7247.0
33	8799.0
34	15785.0
35	32342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.396053252076754	17.44235257131111	11.551250891766726	24.610343284845406
2	13.40546421663443	21.577611218568666	37.9110251450677	27.105899419729205
3	19.213752085297745	28.454341045912816	28.034863760547374	24.297043108242065
4	12.33635143793141	17.748389202519252	34.146488884590745	35.76877047495859
5	11.789949590803042	39.100371118068736	34.44144917374853	14.668230117379688
6	32.752680632955766	33.35227204042406	19.19417816085007	14.7008691657701
7	27.131511187940475	30.77136951029339	22.999649432443213	19.09746986932292
8	28.729615705426543	32.46980888023887	21.07757213834121	17.723003275993378
9	28.470921025591434	13.794228932703117	18.74811116618111	38.98673887552434
10	15.403213132985991	29.313492015521682	33.01862843465542	22.264666416836913
11	33.2809496754228	22.69864487506498	21.858491592422904	22.16191385708932
12	24.331806148229635	25.505603036640355	30.322884808336255	19.839706006793758
13	31.135234457164273	19.532657181195074	23.977612030511466	25.35449633112919
14	22.790517752015763	21.054603919103513	27.211295528450368	28.943582800430352
15	28.428611148048304	23.95222610398559	24.62313987645516	22.996022871510945
16	22.09206740649404	25.34029641449675	29.983559391697494	22.58407678731172
17	24.488957121961246	25.798145618509967	27.93419000761578	21.77870725191301
18	25.370211428502355	23.274059209651487	29.655597596798955	21.700131765047207
19	23.53396274313069	27.045682579210133	29.459763306456487	19.96059137120269
20	24.093661980344038	24.446647244418116	29.29656806450443	22.16312271073341
21	22.523361096672026	25.766715423763646	29.04512650653385	22.664796973030477
22	22.945251018459196	26.067719981141884	25.136902675193113	25.850126325205807
23	20.933718554694583	27.276573625231194	26.98644875064976	24.803259069424467
24	21.822489785063222	29.66320930345011	26.462126157491355	22.05217475399531
25	23.440881012535815	23.74551213084632	25.72803210715279	27.08557474946508
26	23.70561996059137	24.63522841289605	28.05144881109244	23.607702815420136
27	24.07582021711274	24.611348855201758	25.96044583061338	25.352385097072123
28	22.40691489361702	26.043278529980658	28.045212765957444	23.504593810444874
29	23.836176154153016	23.189439454565235	29.425915404421985	23.54846898685976
30	22.577759510656044	26.86435453259674	29.882862081887744	20.675023874859473
31	26.56939424343895	22.93678904295057	26.220035540297136	24.273781173313346
32	19.919490347303654	25.763088862831378	29.24096079687632	25.07645999298865
33	22.063996711918087	27.109751822346865	27.80484266769822	23.02140879803682
34	20.97481957859362	26.025410103598755	28.038151421007456	24.961618896800164
35	20.508202071975145	28.255745076943533	29.778900668496068	21.457152182585254
36	24.4937925365376	25.77034198469591	27.77099476566372	21.964870713102766
37	23.19548372278568	26.713247827085574	27.596919840914865	22.494348609213883
38	24.365654050264133	27.11942265149958	26.933259190309826	21.581664107926454
39	24.09274437271826	24.473537873842506	26.788520587026426	24.645197166412807
40	22.73611933803174	27.407129818792843	27.324927770994766	22.53182307218065
41	21.997509761493177	27.391414721419675	26.71929209530602	23.891783421781124
42	24.755802707930368	27.641441005802708	26.744439071566735	20.858317214700193
43	24.58776595744681	25.204303675048358	25.843810444874276	24.36411992263056
44	22.85030403404214	27.558902817905974	27.031829886002345	22.558963262049538
45	26.618391944004543	24.378331983414128	26.336722234982652	22.666553837598673
46	21.3679553202292	26.994028190807768	29.60397475883078	22.03404173013225
47	24.809303337644913	26.27564280792524	27.486914159302735	21.42813969512711
48	24.241398486423755	26.642326942140766	26.95181218114558	22.164462390289902
49	22.570388907022572	28.045551807928042	28.575055307728576	20.80900397732081
50	22.60973634264576	28.496995926064724	26.29803798309982	22.5952297481897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	53.0
2	46.0
3	150.0
4	254.0
5	216.5
6	179.0
7	148.0
8	117.0
9	141.5
10	166.0
11	210.0
12	254.0
13	389.5
14	525.0
15	608.5
16	692.0
17	676.0
18	660.0
19	615.0
20	570.0
21	607.5
22	645.0
23	664.5
24	684.0
25	745.5
26	807.0
27	998.0
28	1189.0
29	1230.0
30	1271.0
31	1395.0
32	1519.0
33	1714.0
34	1909.0
35	2144.0
36	2379.0
37	2578.0
38	2777.0
39	2921.5
40	3066.0
41	3303.0
42	3540.0
43	4131.0
44	4722.0
45	5489.5
46	6257.0
47	7409.0
48	8561.0
49	8955.5
50	9350.0
51	8154.5
52	6959.0
53	5904.5
54	4850.0
55	4562.5
56	4275.0
57	4088.5
58	3902.0
59	3480.5
60	3059.0
61	2740.0
62	2421.0
63	2088.0
64	1755.0
65	1487.5
66	1220.0
67	997.5
68	775.0
69	601.0
70	427.0
71	358.0
72	289.0
73	239.5
74	190.0
75	163.0
76	136.0
77	116.0
78	96.0
79	80.5
80	65.0
81	50.0
82	35.0
83	30.0
84	25.0
85	25.5
86	26.0
87	18.0
88	10.0
89	7.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026594780169964822
2	0.00362656093226793
3	0.0012088536440893103
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0012088536440893103
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0012088536440893103
25	0.0
26	0.0
27	0.0012088536440893103
28	0.00362656093226793
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0012088536440893103
40	0.0
41	0.0
42	0.00362656093226793
43	0.00362656093226793
44	0.0024177072881786206
45	0.0024177072881786206
46	0.0012088536440893103
47	0.0
48	0.006044268220446551
49	0.004835414576357241
50	0.0024177072881786206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	82723.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.48983958512143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.47683121412005	62.08188774585061
2	10.812100077311532	15.89159000519807
3	2.549635648841149	5.621169445015292
4	0.8833253828565788	2.596617627503838
5	0.4178112611649368	1.535244127993424
6	0.23686937640846809	1.044449548493164
7	0.1348839504548221	0.693881991707264
8	0.0773115325777639	0.4545289701775806
9	0.06086227032717583	0.4025482634817403
>10	0.30266642541082034	4.062957097784172
>50	0.021384040925764482	1.028734451120003
>100	0.023028967150823285	2.9822419399683278
>500	0.0032898524501176122	1.6041487857065144
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	788	0.9525766715423764	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	539	0.6515721141641382	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	323	0.3904597270408472	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	246	0.2973779964459703	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	243	0.29375143551370236	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	217	0.2623212407673803	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	174	0.21034053407153996	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	172	0.20792282678336135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	169	0.2042962658510934	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	162	0.19583429034246824	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	161	0.19462543669837892	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	144	0.17407492474886066	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	130	0.15715097373161033	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	117	0.1414358763584493	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.12934733991755618	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.12330307169710963	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	97	0.11725880347666308	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	86	0.10396141339168068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.00120885364408931
14	0.0	0.0	0.0	0.0	0.00120885364408931
15	0.0	0.0	0.0	0.0	0.00120885364408931
16	0.0	0.0	0.0	0.0	0.00120885364408931
17	0.0	0.0	0.0	0.0	0.00120885364408931
18	0.0	0.0	0.0	0.0	0.00120885364408931
19	0.0	0.0	0.0	0.0	0.00120885364408931
20	0.0	0.0	0.0	0.0	0.00120885364408931
21	0.0	0.0	0.0	0.0	0.00120885364408931
22	0.0	0.0	0.0	0.00120885364408931	0.00120885364408931
23	0.0	0.0	0.0	0.00120885364408931	0.00120885364408931
24	0.0	0.0	0.0	0.00120885364408931	0.00120885364408931
25	0.0	0.0	0.0	0.00120885364408931	0.00120885364408931
26	0.0	0.0	0.0	0.00241770728817862	0.00120885364408931
27	0.0	0.0	0.0	0.0036265609322679304	0.00120885364408931
28	0.0	0.0	0.0	0.008461975508625172	0.00120885364408931
29	0.0	0.0	0.0	0.00967082915271448	0.00120885364408931
30	0.0	0.0	0.0	0.01571509737316103	0.00120885364408931
31	0.0	0.0	0.0	0.02296821923769689	0.00120885364408931
32	0.0	0.0	0.0	0.030221341102232752	0.00120885364408931
33	0.0	0.0	0.0	0.043518731187215165	0.00120885364408931
34	0.0	0.0	0.0	0.05560726762810826	0.00120885364408931
35	0.0	0.0	0.0	0.07857548686580516	0.00120885364408931
36	0.0	0.0	0.0	0.09912599881532343	0.00120885364408931
37	0.0	0.0	0.0	0.1196765107648417	0.00120885364408931
38	0.0	0.0	0.0	0.1668218028843248	0.00120885364408931
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGAG	25	0.0023409706	35.197582	5
GGGCCTG	25	0.0023409706	35.197582	5
TATAAGA	35	3.1821715E-4	31.445425	2
GGAATAT	30	0.005716435	29.33132	12
TATGGCG	30	0.005716435	29.33132	16
AGAGTGA	30	0.005716435	29.33132	8
AAAGTAC	45	0.0013877985	24.442766	3
ATATACA	50	0.0025523829	22.011797	2
AAGTACT	60	2.8428884E-4	21.99849	4
ATATGGC	50	0.002561375	21.99849	15
TATACAC	50	0.002561375	21.99849	3
CAGAGGA	50	0.002561375	21.99849	4
GCTGGAC	65	4.8812066E-4	20.3063	3
GAATATG	55	0.0044507636	19.998627	13
TGGACCC	70	8.039987E-4	18.855848	5
AAATGAG	60	0.0073325895	18.343164	43
CTGAAAA	75	0.0012777209	17.598791	29
GCTTCCA	175	1.0197582E-6	13.827621	27
TGCTTCC	805	0.0	13.663656	26
ACCACTG	830	0.0	13.252102	21
>>END_MODULE