FastQCFastQC Report
Thu 2 Feb 2017
SRR4062280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081014
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33360.3085991485771692No Hit
TATCAACGCAGAGTACTTTTTTTTT22350.20675032885790565No Hit
GGTATCAACGCAGAGTACTTTTTTT14110.13052559911342498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11950.11054435927749316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG704.4235094E-714.9311935
TTAGACT801.9980052E-613.0623734
GCGTTAT751.4833819E-512.661851
GTCTTAA604.1033706E-412.661851
CTTAGAC1155.320544E-912.390613
TCGCGTA1650.012.0941529
CGGTTCT1650.012.09247112
CGCATCG1103.8091457E-812.09079113
GTTTTCG1103.8115104E-812.09023115
TTTTCGG1103.8164217E-812.0891116
TCCCTAT550.003076151212.0863111
ATTATAC951.0394888E-611.9993273
TACCGCG1459.822543E-1111.790256517
CGGTCCA1052.7088936E-711.76451510
CGCGTAA1701.8189894E-1211.73789810
ATTACCG1501.7644197E-1011.39936115
CCGTCAA1751.8189894E-1211.39724918
CCGGTCC1354.6984496E-911.262289
TGTACCG855.3096293E-511.1784335
GTATCAA19350.011.1404651
Produced by FastQC (version 0.11.5)