##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062280_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1081014 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26360620676513 32.0 32.0 32.0 32.0 32.0 2 30.888369623335127 32.0 32.0 32.0 32.0 32.0 3 30.91670690666356 32.0 32.0 32.0 32.0 32.0 4 30.902430495812265 32.0 32.0 32.0 32.0 32.0 5 30.86892029150409 32.0 32.0 32.0 32.0 32.0 6 34.51453080163624 36.0 36.0 36.0 32.0 36.0 7 34.44465844105627 36.0 36.0 36.0 32.0 36.0 8 34.39185431456022 36.0 36.0 36.0 32.0 36.0 9 34.487455296601155 36.0 36.0 36.0 32.0 36.0 10 34.23835213975027 36.0 36.0 36.0 32.0 36.0 11 34.49446445651953 36.0 36.0 36.0 32.0 36.0 12 34.34288085075679 36.0 36.0 36.0 32.0 36.0 13 34.42216289520765 36.0 36.0 36.0 32.0 36.0 14 34.33395867213561 36.0 36.0 36.0 32.0 36.0 15 34.3079562336843 36.0 36.0 36.0 32.0 36.0 16 34.307351246144826 36.0 36.0 36.0 32.0 36.0 17 34.24191638591174 36.0 36.0 36.0 32.0 36.0 18 34.251978235249496 36.0 36.0 36.0 32.0 36.0 19 34.24257872701001 36.0 36.0 36.0 32.0 36.0 20 34.20987517275447 36.0 36.0 36.0 32.0 36.0 21 34.19557748558298 36.0 36.0 36.0 32.0 36.0 22 34.17494593039498 36.0 36.0 36.0 32.0 36.0 23 34.16737156040532 36.0 36.0 36.0 32.0 36.0 24 34.13776139809475 36.0 36.0 36.0 32.0 36.0 25 33.80639751196562 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 13.0 5 78.0 6 221.0 7 46.0 8 165.0 9 146.0 10 72.0 11 23.0 12 45.0 13 50.0 14 229.0 15 322.0 16 474.0 17 598.0 18 805.0 19 1093.0 20 1608.0 21 2171.0 22 3143.0 23 4411.0 24 5767.0 25 8349.0 26 11169.0 27 14474.0 28 19176.0 29 25146.0 30 33824.0 31 45846.0 32 66589.0 33 96889.0 34 220829.0 35 517242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.412320398444415 17.250537463321137 12.003378340875704 25.33376379735875 2 17.097698358296075 19.04356199890658 36.958273320067974 26.900466322729372 3 19.376840297981417 22.72964004858433 27.740615925706308 30.152903727727942 4 13.483331097552515 15.109283424537773 33.8935429108016 37.51384256710812 5 15.596805685361858 35.786595352882934 32.46300905919476 16.153589902560448 6 35.71669889307159 33.862623880367686 16.010429692598045 14.41024753396268 7 31.326457006288326 29.874890850114404 19.547529161423306 19.25112298217396 8 28.864168401401617 32.411361834843355 18.505207908909746 20.21926185484528 9 27.06782654438125 14.579414671100949 18.50395779097196 39.848800993545844 10 17.26122331025131 26.46467116451222 29.730333040639923 26.543772484596545 11 37.38690711631789 21.43741784642063 21.179713820698396 19.99596121656309 12 25.038091859503332 23.49376219749969 27.638619634373722 23.829526308623254 13 29.6354441634944 19.433715857623163 24.73144571952541 26.199394259357028 14 23.913546793011285 19.373067925667577 24.563853609656622 32.14953167166452 15 25.43100214424319 26.900943374381157 21.877578996605333 25.790475484770315 16 26.131705537063482 25.75948698855838 22.99021432897859 25.118593145399547 17 24.306314483116306 25.922814751699484 24.681404782632857 25.089465982551356 18 25.052645615828688 24.96707912677731 25.548542313740093 24.431732943653913 19 25.64155508415332 24.792568099978425 25.06081057932783 24.505066236540422 20 26.2218173030718 24.039883301807045 24.5822650967408 25.15603429838035 21 26.909024919124413 23.94241399041159 24.539050825236515 24.60951026522748 22 26.40194747592971 23.739076004526087 24.896153753557996 24.96282276598621 23 24.9120978209147 24.087326348064856 25.51709070171768 25.483485129302764 24 25.03428078859239 24.66827955346553 25.225475463658658 25.07196419428342 25 25.07073392932532 24.236369831053306 25.086936072822617 25.605960166798752 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 346.0 3 528.0 4 528.0 5 528.0 6 1085.0 7 1642.0 8 1642.0 9 1642.0 10 1831.5 11 2021.0 12 2021.0 13 2021.0 14 2353.5 15 2686.0 16 2686.0 17 2686.0 18 4446.0 19 6206.0 20 6206.0 21 6206.0 22 9646.5 23 13087.0 24 13087.0 25 13087.0 26 19247.0 27 25407.0 28 25407.0 29 25407.0 30 32077.5 31 38748.0 32 38748.0 33 38748.0 34 48704.5 35 58661.0 36 58661.0 37 58661.0 38 70045.5 39 81430.0 40 81430.0 41 81430.0 42 95559.0 43 109688.0 44 109688.0 45 109688.0 46 123626.5 47 137565.0 48 137565.0 49 137565.0 50 143626.0 51 149687.0 52 149687.0 53 149687.0 54 142726.5 55 135766.0 56 135766.0 57 135766.0 58 125836.5 59 115907.0 60 115907.0 61 115907.0 62 102431.0 63 88955.0 64 88955.0 65 88955.0 66 72249.0 67 55543.0 68 55543.0 69 55543.0 70 42395.5 71 29248.0 72 29248.0 73 29248.0 74 21880.5 75 14513.0 76 14513.0 77 14513.0 78 11366.0 79 8219.0 80 8219.0 81 8219.0 82 5651.0 83 3083.0 84 3083.0 85 3083.0 86 2184.5 87 1286.0 88 1286.0 89 1286.0 90 870.0 91 454.0 92 454.0 93 454.0 94 285.0 95 116.0 96 116.0 97 116.0 98 260.0 99 404.0 100 404.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.250573998116584E-4 2 2.7751721994349746E-4 3 6.475401798681608E-4 4 0.010083125657947075 5 0.031821974553521044 6 0.058926156368002636 7 0.09962868195971561 8 0.10342141729894341 9 0.11516964627655145 10 0.12821295561389584 11 0.1370010009121066 12 0.12830546135387702 13 0.12432771453468687 14 0.11248697981709765 15 0.1275654154340277 16 0.11766730125604292 17 0.11785231273600527 18 0.10656661245830304 19 0.09444836052077031 20 0.0890830276018627 21 0.0888055103819192 22 0.09731603846018645 23 0.07733479862425463 24 0.0890830276018627 25 0.08418022338286091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1081014.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.02485192271131 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23939294932197 43.83350404508587 2 12.673999212279469 14.201178582730343 3 3.8979249526809956 6.551420048393829 4 1.69128278162997 3.7901546960100148 5 0.93159905878819 2.609634965997279 6 0.5582735773108218 1.876631670072067 7 0.38047767000150784 1.4921343585212887 8 0.2750227701501884 1.2326487978430538 9 0.2039489079015229 1.0283586628483357 >10 0.9995679083401382 9.973744708869816 >50 0.07766960615624866 3.054163355858601 >100 0.06420448426750999 7.217794293890702 >500 0.005640702995851327 2.183602144144027 >1k 9.954181757384694E-4 0.9550296697348094 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3336 0.3085991485771692 No Hit TATCAACGCAGAGTACTTTTTTTTT 2235 0.20675032885790565 No Hit GGTATCAACGCAGAGTACTTTTTTT 1411 0.13052559911342498 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1195 0.11054435927749316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 9.250573998116583E-5 13 0.0 0.0 0.0 0.0 9.250573998116583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTG 70 4.4235094E-7 14.931193 5 TTAGACT 80 1.9980052E-6 13.062373 4 GCGTTAT 75 1.4833819E-5 12.66185 1 GTCTTAA 60 4.1033706E-4 12.66185 1 CTTAGAC 115 5.320544E-9 12.39061 3 TCGCGTA 165 0.0 12.094152 9 CGGTTCT 165 0.0 12.092471 12 CGCATCG 110 3.8091457E-8 12.090791 13 GTTTTCG 110 3.8115104E-8 12.090231 15 TTTTCGG 110 3.8164217E-8 12.08911 16 TCCCTAT 55 0.0030761512 12.086311 1 ATTATAC 95 1.0394888E-6 11.999327 3 TACCGCG 145 9.822543E-11 11.7902565 17 CGGTCCA 105 2.7088936E-7 11.764515 10 CGCGTAA 170 1.8189894E-12 11.737898 10 ATTACCG 150 1.7644197E-10 11.399361 15 CCGTCAA 175 1.8189894E-12 11.397249 18 CCGGTCC 135 4.6984496E-9 11.26228 9 TGTACCG 85 5.3096293E-5 11.178433 5 GTATCAA 1935 0.0 11.140465 1 >>END_MODULE