FastQCFastQC Report
Thu 2 Feb 2017
SRR4062280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081014
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.1221075767751389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.023.4614161
GGTATCA10550.020.6513021
AAAGCGT654.9331324E-420.3068166
TCTAGCG2000.018.69919428
GATAGTC851.4311279E-418.1168677
CTAGCGG2100.017.80875629
CTAGGAC2003.6379788E-1217.5992413
TACGAAT2150.017.39701341
ATACGAA2150.017.39701340
GGTCTAC1651.6516424E-917.3391
CAAGCCG1154.271911E-617.21744735
TACGCTA2300.017.216659
AACGCAG31700.017.0024226
CGCAATA2350.016.85111636
TAGGACA2350.016.8503384
CAATACG2500.016.721638
TAGCGGC2251.8189894E-1216.62150630
ACGCTAT2400.016.49928910
ACCGTCG2800.016.4992898
TATCAAC33100.016.416212
Produced by FastQC (version 0.11.5)