##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081014
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29135145335768	32.0	32.0	32.0	32.0	32.0
2	31.493433942576136	32.0	32.0	32.0	32.0	32.0
3	31.598815556505283	32.0	32.0	32.0	32.0	32.0
4	31.67408007666876	32.0	32.0	32.0	32.0	32.0
5	31.652855559687477	32.0	32.0	32.0	32.0	32.0
6	35.317355741923784	36.0	36.0	36.0	36.0	36.0
7	35.31262592343855	36.0	36.0	36.0	36.0	36.0
8	35.27296038719202	36.0	36.0	36.0	36.0	36.0
9	35.34143591109829	36.0	36.0	36.0	36.0	36.0
10	35.24249547184403	36.0	36.0	36.0	36.0	36.0
11	35.34660975713543	36.0	36.0	36.0	36.0	36.0
12	35.27804542771879	36.0	36.0	36.0	36.0	36.0
13	35.30048362000862	36.0	36.0	36.0	36.0	36.0
14	35.26242953375257	36.0	36.0	36.0	36.0	36.0
15	35.252665552897554	36.0	36.0	36.0	36.0	36.0
16	35.258809784147104	36.0	36.0	36.0	36.0	36.0
17	35.24211712336751	36.0	36.0	36.0	36.0	36.0
18	35.238288310789684	36.0	36.0	36.0	36.0	36.0
19	35.222881479795824	36.0	36.0	36.0	36.0	36.0
20	35.214160038630396	36.0	36.0	36.0	36.0	36.0
21	35.20428412583001	36.0	36.0	36.0	36.0	36.0
22	35.19909270370226	36.0	36.0	36.0	36.0	36.0
23	35.17504861176636	36.0	36.0	36.0	36.0	36.0
24	35.15774078781588	36.0	36.0	36.0	36.0	36.0
25	35.14999805737946	36.0	36.0	36.0	36.0	36.0
26	35.10875992355326	36.0	36.0	36.0	36.0	36.0
27	35.09867587283791	36.0	36.0	36.0	36.0	36.0
28	35.08563163844317	36.0	36.0	36.0	36.0	36.0
29	35.062791046184415	36.0	36.0	36.0	36.0	36.0
30	35.04527230914678	36.0	36.0	36.0	36.0	36.0
31	35.049432292273735	36.0	36.0	36.0	36.0	36.0
32	35.028126370241274	36.0	36.0	36.0	36.0	36.0
33	34.98982621871687	36.0	36.0	36.0	36.0	36.0
34	34.968251104981064	36.0	36.0	36.0	36.0	36.0
35	34.96264340702341	36.0	36.0	36.0	36.0	36.0
36	34.95132995502371	36.0	36.0	36.0	36.0	36.0
37	34.935631730948906	36.0	36.0	36.0	36.0	36.0
38	34.917548708897385	36.0	36.0	36.0	36.0	36.0
39	34.90104660994215	36.0	36.0	36.0	36.0	36.0
40	34.88295711248883	36.0	36.0	36.0	36.0	36.0
41	34.85088167220776	36.0	36.0	36.0	32.0	36.0
42	34.82285428310826	36.0	36.0	36.0	32.0	36.0
43	34.80320328876407	36.0	36.0	36.0	32.0	36.0
44	34.75960070822394	36.0	36.0	36.0	32.0	36.0
45	34.730738917349825	36.0	36.0	36.0	32.0	36.0
46	34.696186173352054	36.0	36.0	36.0	32.0	36.0
47	34.670446451202295	36.0	36.0	36.0	32.0	36.0
48	34.63209727163571	36.0	36.0	36.0	32.0	36.0
49	34.608068905675594	36.0	36.0	36.0	32.0	36.0
50	34.199491403441584	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	38.0
22	73.0
23	211.0
24	520.0
25	1193.0
26	2397.0
27	4775.0
28	8100.0
29	12664.0
30	19410.0
31	28315.0
32	42465.0
33	72173.0
34	166031.0
35	722638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06702181901296	17.74484737610152	12.417839789092437	25.770291015793084
2	16.557613478992497	19.482113766234246	36.62660700425821	27.333665750515046
3	18.72752686600439	23.012674223159205	28.193555489156928	30.066243421679477
4	13.065615307123965	15.463551317144198	34.13668475772262	37.334148618009216
5	15.383704558867878	36.068358041616484	32.17127622768993	16.376661171825713
6	35.86583682697324	33.97418345032248	15.852275460401918	14.307704262302362
7	31.3472350959377	29.97657754663677	19.54396520304085	19.132222154384678
8	29.010262586793512	32.410773588501165	18.421778071329324	20.157185753375998
9	27.235632470994826	14.349767903098387	18.40281439463319	40.0117852312736
10	17.26954254944205	26.490615746526636	29.720456645757267	26.519385058274047
11	37.5663034891315	21.334506306116293	21.186959650846333	19.912230553905868
12	25.014222770678984	23.505360250061745	27.72085072057413	23.75956625868514
13	29.805164410451667	19.34322774728172	24.89940000777048	25.952207834496132
14	24.052139935282984	19.155071072160027	24.680623932715026	32.11216505984196
15	25.53935471696019	26.78198432212719	21.943101569452384	25.73555939146024
16	26.260538794419276	25.63926564699918	23.033729692527146	25.0664658660544
17	24.56822945863791	25.80688131698572	24.57081961935738	25.05406960501899
18	25.13297700122293	24.75943882317898	25.722423576382912	24.38516059921518
19	25.811044075284872	24.562494102759075	25.233623246322434	24.39283857563362
20	26.281484386852323	23.81758944396547	24.8785397386893	25.02238643049291
21	27.083368022985827	23.707001019413255	24.604491708710523	24.605139248890392
22	26.520840618160356	23.53919560708742	25.057399811658314	24.882563963093908
23	24.924214602414587	23.907945602874342	25.657785799060694	25.510053995650377
24	25.10825594378554	24.33147734095095	25.48124288276721	25.0790238324963
25	25.284339781297728	23.887871838729044	25.348353812581347	25.479434567391877
26	24.97016678846634	24.783119490106476	25.603185909473545	24.643527811953636
27	25.395277758248753	24.285761860182866	25.213503642882777	25.1054567386856
28	25.07779833487512	24.15013876040703	25.636817761332097	25.135245143385752
29	24.85802642156278	24.46247077966829	25.745436447917736	24.934066350851193
30	24.92990280287031	24.522224349883764	25.614639791526173	24.933233055719757
31	25.53028915444203	24.29237734201407	24.758513765779167	25.418819737764732
32	25.41859079673786	24.256527241241507	24.85097242573598	25.47390953628465
33	24.953377020791628	24.083358926635412	25.29740650427562	25.66585754829734
34	25.678049592740088	24.23060022849108	25.26130776453393	24.830042414234903
35	25.986531114420774	24.15750085568126	25.14574333262412	24.710224697273848
36	24.840797326949193	24.708328862214294	25.173818607008986	25.277055203827526
37	25.805920444033305	24.377798334875113	24.880296022201666	24.935985198889917
38	25.082283855713243	24.302552973412002	25.35785845511714	25.257304715757613
39	25.573908796234623	24.313233574589642	24.853655107085242	25.259202522090497
40	25.767658883233707	24.42068280336795	25.06831548897609	24.743342824422253
41	24.90531967445139	24.877012708532607	25.39495617970668	24.822711437309323
42	26.062353083749546	24.743541235535023	24.819676347142806	24.37442933357262
43	25.551009231515	24.124051431537016	24.982446337598326	25.34249299934966
44	25.12555748076512	24.6742998707653	24.841185628953014	25.35895701951657
45	25.330044986581733	24.870095810410653	24.612923905793675	25.186935297213942
46	25.14805762822873	24.644911461445812	24.860638040033376	25.346392870292085
47	25.344472391792422	24.625694148294038	25.068339437243814	24.96149402266973
48	25.878119290455377	25.05904391868452	24.154767686579245	24.90806910428086
49	25.30840270756069	25.22754962427369	24.232335127704392	25.23171254046123
50	25.440425531914894	25.19935245143386	24.300555041628122	25.059666975023127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	46.5
2	59.0
3	147.0
4	235.0
5	232.0
6	229.0
7	199.5
8	170.0
9	232.0
10	294.0
11	538.0
12	782.0
13	1452.0
14	2122.0
15	2867.5
16	3613.0
17	3717.5
18	3822.0
19	3653.0
20	3484.0
21	3617.0
22	3750.0
23	4061.0
24	4372.0
25	4882.0
26	5392.0
27	6484.0
28	7576.0
29	8889.5
30	10203.0
31	12076.5
32	13950.0
33	16270.5
34	18591.0
35	21584.5
36	24578.0
37	28709.0
38	32840.0
39	37149.5
40	41459.0
41	45558.0
42	49657.0
43	52661.0
44	55665.0
45	62146.0
46	68627.0
47	73524.0
48	78421.0
49	82135.5
50	85850.0
51	84416.0
52	82982.0
53	83304.5
54	83627.0
55	84124.5
56	84622.0
57	82171.0
58	79720.0
59	73096.5
60	66473.0
61	58791.0
62	51109.0
63	44377.5
64	37646.0
65	31675.5
66	25705.0
67	22178.5
68	18652.0
69	16368.5
70	14085.0
71	10755.0
72	7425.0
73	6534.0
74	5643.0
75	4200.0
76	2757.0
77	2345.0
78	1933.0
79	1564.5
80	1196.0
81	962.5
82	729.0
83	626.5
84	524.0
85	388.0
86	252.0
87	167.0
88	82.0
89	59.0
90	36.0
91	23.5
92	11.0
93	11.0
94	11.0
95	10.5
96	10.0
97	6.5
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03246951473338921
2	0.0039777468191901305
3	9.250573998116583E-5
4	9.250573998116583E-5
5	0.0
6	1.8501147996233166E-4
7	0.0
8	0.0
9	0.0
10	9.250573998116583E-5
11	0.0
12	9.250573998116583E-5
13	0.0
14	0.0
15	0.0
16	7.400459198493266E-4
17	0.0
18	0.0
19	0.0
20	1.8501147996233166E-4
21	0.0
22	0.0
23	9.250573998116583E-5
24	0.0010175631397928242
25	9.250573998116583E-5
26	3.700229599246633E-4
27	1.8501147996233166E-4
28	0.0012950803597363216
29	2.7751721994349746E-4
30	0.0013875860997174874
31	0.0
32	5.550344398869949E-4
33	1.8501147996233166E-4
34	8.325516598304925E-4
35	3.700229599246633E-4
36	1.8501147996233166E-4
37	0.0012950803597363216
38	0.0
39	5.550344398869949E-4
40	0.0
41	7.400459198493266E-4
42	0.003792735339227799
43	0.0039777468191901305
44	0.003237700899340804
45	0.0023126434995291456
46	0.0014800918396986533
47	0.0012025746197551557
48	0.0036077238592654676
49	0.003792735339227799
50	0.0012950803597363216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1081014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84488036122498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21985442414821	45.246181210372875
2	13.03739591546916	15.082932139044736
3	3.8036459188817426	6.600643293425282
4	1.6223172305551494	3.7537098443766586
5	0.8843750537929709	2.5578284590553153
6	0.5516561592502065	1.914629071941664
7	0.34733136773880957	1.406393898878645
8	0.25039208321369305	1.158712007751517
9	0.19732278365417033	1.0272701531717425
>10	0.9512766001321584	9.712832541356763
>50	0.07101483197547975	2.8465646099562267
>100	0.058266962092686794	6.513990132888887
>500	0.0046677938679202365	1.8666861888718305
>1k	4.828752277158866E-4	0.31162644890787655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1320	0.1221075767751389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.250573998116583E-5	0.0	0.0	0.0	0.0
7	9.250573998116583E-5	0.0	0.0	0.0	0.0
8	9.250573998116583E-5	0.0	0.0	0.0	0.0
9	9.250573998116583E-5	0.0	0.0	0.0	0.0
10	9.250573998116583E-5	0.0	0.0	0.0	0.0
11	9.250573998116583E-5	0.0	0.0	0.0	0.0
12	9.250573998116583E-5	0.0	0.0	0.0	0.0
13	9.250573998116583E-5	0.0	0.0	0.0	9.250573998116583E-5
14	2.775172199434975E-4	0.0	0.0	0.0	9.250573998116583E-5
15	2.775172199434975E-4	0.0	0.0	0.0	9.250573998116583E-5
16	2.775172199434975E-4	0.0	0.0	0.0	9.250573998116583E-5
17	2.775172199434975E-4	0.0	0.0	0.0	9.250573998116583E-5
18	2.775172199434975E-4	0.0	0.0	0.0	9.250573998116583E-5
19	5.55034439886995E-4	0.0	0.0	0.0	9.250573998116583E-5
20	5.55034439886995E-4	0.0	0.0	0.0	1.8501147996233166E-4
21	5.55034439886995E-4	0.0	0.0	9.250573998116583E-5	1.8501147996233166E-4
22	5.55034439886995E-4	0.0	0.0	1.8501147996233166E-4	1.8501147996233166E-4
23	5.55034439886995E-4	0.0	0.0	5.55034439886995E-4	2.775172199434975E-4
24	5.55034439886995E-4	0.0	0.0	8.325516598304925E-4	2.775172199434975E-4
25	5.55034439886995E-4	0.0	0.0	0.00111006887977399	2.775172199434975E-4
26	5.55034439886995E-4	0.0	0.0	0.0012025746197551557	2.775172199434975E-4
27	5.55034439886995E-4	0.0	0.0	0.0014800918396986533	2.775172199434975E-4
28	8.325516598304925E-4	0.0	0.0	0.00222013775954798	2.775172199434975E-4
29	9.250573998116584E-4	0.0	0.0	0.0031451951593596383	2.775172199434975E-4
30	9.250573998116584E-4	0.0	0.0	0.0062903903187192765	2.775172199434975E-4
31	9.250573998116584E-4	0.0	0.0	0.011840734717589226	2.775172199434975E-4
32	9.250573998116584E-4	0.0	0.0	0.017483584856440342	2.775172199434975E-4
33	9.250573998116584E-4	0.0	0.0	0.021923860375536303	2.775172199434975E-4
34	9.250573998116584E-4	0.0	0.0	0.028121744954274414	4.625286999058292E-4
35	0.0010175631397928242	0.0	0.0	0.03644726155257934	4.625286999058292E-4
36	0.00111006887977399	0.0	0.0	0.05060063976969771	4.625286999058292E-4
37	0.00111006887977399	0.0	0.0	0.06586408686659007	4.625286999058292E-4
38	0.0012025746197551557	0.0	0.0	0.08704790132227705	4.625286999058292E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2270	0.0	23.461416	1
GGTATCA	1055	0.0	20.651302	1
AAAGCGT	65	4.9331324E-4	20.306816	6
TCTAGCG	200	0.0	18.699194	28
GATAGTC	85	1.4311279E-4	18.116867	7
CTAGCGG	210	0.0	17.808756	29
CTAGGAC	200	3.6379788E-12	17.599241	3
TACGAAT	215	0.0	17.397013	41
ATACGAA	215	0.0	17.397013	40
GGTCTAC	165	1.6516424E-9	17.339	1
CAAGCCG	115	4.271911E-6	17.217447	35
TACGCTA	230	0.0	17.21665	9
AACGCAG	3170	0.0	17.002422	6
CGCAATA	235	0.0	16.851116	36
TAGGACA	235	0.0	16.850338	4
CAATACG	250	0.0	16.7216	38
TAGCGGC	225	1.8189894E-12	16.621506	30
ACGCTAT	240	0.0	16.499289	10
ACCGTCG	280	0.0	16.499289	8
TATCAAC	3310	0.0	16.41621	2
>>END_MODULE