FastQCFastQC Report
Thu 2 Feb 2017
SRR4062279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30241
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1210.4001190436824179No Hit
TATCAACGCAGAGTACTTTTTTTTT820.27115505439634935No Hit
GGTATCAACGCAGAGTACTTTTTTT570.18848583049502332No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT540.1785655236268642No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG460.15211137197843988No Hit
CTCCACTCCTGGTGGTGCCCTTCCG440.1454978340663338No Hit
ACCATACTCCCCCCGGAACCCAAAG430.14219106511028076No Hit
GTCAATCCTGTCCGTGTCCGGGCCG420.1388842961542277No Hit
GGCTCCACTCCTGGTGGTGCCCTTC400.13227075824212162No Hit
TCGTAGTTCCGACCATAAACGATGC350.11573691346185642No Hit
GAATAACGCCGCCGCATCGCCAGTC330.10912337554975034No Hit
ACGCAGAGTACTTTTTTTTTTTTTT320.10581660659369731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA551.0571275E-515.5454551
ATCAACG751.9485969E-411.4000013
AACGCAG700.001419438910.8571436
TCAACGC700.001419438910.8571434
CAACGCA700.001419438910.8571435
TATCAAC803.5455165E-410.68752
ACGCAGA750.002521409210.1333347
CAGAGTA750.002521409210.13333410
GCAGAGT750.002521409210.1333349
CGCAGAG750.002521409210.1333348
AGAGTAC856.2036386E-410.05882411
Produced by FastQC (version 0.11.5)