FastQCFastQC Report
Thu 2 Feb 2017
SRR4062279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30241
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC500.16533844780265203No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG470.1554181409344929No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG440.1454978340663338No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC410.13557752719817467No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA390.1289639892860686No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG380.12565722033001553No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT360.11904368241790945No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT340.11243014450580337No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT330.10912337554975034No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG330.10912337554975034No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA330.10912337554975034No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT320.10581660659369731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA403.9498627E-738.51
TAGGAAT453.9412465E-529.33333432
GCTAGGA453.9412465E-529.33333430
AGCTAGG453.9412465E-529.33333429
CTAGGAA453.9412465E-529.33333431
GCAGCTA453.9412465E-529.33333427
CCCCGGA300.005657895529.3333321
TTGGACC300.005657895529.33333230
GCAGGCC508.0802085E-526.43
AGGAATA508.0802085E-526.433
AGCAGGC508.0802085E-526.42
TGGATAC508.0802085E-526.419
ATCAACG609.348858E-625.6666663
CCGCAGC450.001366937424.44444525
GGATACC450.001366937424.44444520
TACCGCA450.001366937424.44444523
CAGCTAG551.5440467E-424.028
CGCCTGG551.5440467E-424.015
CCGCCTG551.5440467E-424.014
ATAATGG551.5440467E-424.037
Produced by FastQC (version 0.11.5)