FastQCFastQC Report
Thu 2 Feb 2017
SRR4062278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181668
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21140.1788996570948862No Hit
TATCAACGCAGAGTACTTTTTTTTT16030.13565570024744683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.014.2944131
TAGGACA954.8967195E-914.04
CTACACG707.258999E-613.5714284
GCGTTAT802.0087227E-613.0564141
CCGTCGT1550.012.873159
GTATCAA13450.012.7078311
GTGTAGG1052.0045263E-812.6607661
GTCCTAA604.1068436E-412.6607651
CGTTATT853.9522583E-612.2909932
CGTCGTA1551.8189894E-1212.25962410
CGGACAT1251.4078978E-912.1605165
CACGCCC1201.0013537E-811.87399316
GGTTTAT802.878688E-511.8694671
TTAGACA658.0209115E-411.6923074
ACGCCCC1251.8317223E-811.399034517
ACTCTAA1604.5474735E-1111.28268410
GTCGGCG1104.9555456E-711.22870112
AGAACCG1104.9609844E-711.2277495
AAGACGG2800.011.1969035
AACCGCG855.310928E-511.1783667
Produced by FastQC (version 0.11.5)