##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062278_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181668
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227206795817438	32.0	32.0	32.0	32.0	32.0
2	30.70963333186648	32.0	32.0	32.0	32.0	32.0
3	30.73509733698467	32.0	32.0	32.0	32.0	32.0
4	30.746190131238215	32.0	32.0	32.0	32.0	32.0
5	30.639001817769458	32.0	32.0	32.0	32.0	32.0
6	34.301742959951525	36.0	36.0	36.0	32.0	36.0
7	34.24320282854406	36.0	36.0	36.0	32.0	36.0
8	34.18532616606356	36.0	36.0	36.0	32.0	36.0
9	34.343665902774724	36.0	36.0	36.0	32.0	36.0
10	33.9891729318218	36.0	36.0	36.0	32.0	36.0
11	34.34577224736559	36.0	36.0	36.0	32.0	36.0
12	34.12241255581094	36.0	36.0	36.0	32.0	36.0
13	34.23393626636246	36.0	36.0	36.0	32.0	36.0
14	34.12407799821947	36.0	36.0	36.0	32.0	36.0
15	34.068449852242765	36.0	36.0	36.0	32.0	36.0
16	34.07610428648317	36.0	36.0	36.0	32.0	36.0
17	34.000842876340904	36.0	36.0	36.0	32.0	36.0
18	34.005917059614035	36.0	36.0	36.0	32.0	36.0
19	34.023848492131464	36.0	36.0	36.0	32.0	36.0
20	34.00836021623671	36.0	36.0	36.0	32.0	36.0
21	33.98980085777054	36.0	36.0	36.0	32.0	36.0
22	33.96431654237908	36.0	36.0	36.0	32.0	36.0
23	33.91830023322964	36.0	36.0	36.0	32.0	36.0
24	33.90109489298179	36.0	36.0	36.0	32.0	36.0
25	33.53968288893327	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	13.0
5	111.0
6	250.0
7	54.0
8	221.0
9	155.0
10	73.0
11	26.0
12	62.0
13	54.0
14	212.0
15	298.0
16	418.0
17	569.0
18	789.0
19	1116.0
20	1630.0
21	2459.0
22	3749.0
23	5360.0
24	7676.0
25	10742.0
26	14576.0
27	18860.0
28	24936.0
29	32563.0
30	42605.0
31	58585.0
32	82613.0
33	116051.0
34	248664.0
35	506178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.964879914696276	17.4166003757426	12.027994516189091	25.590525193372038
2	16.8333379877749	19.1876391572245	37.35248593723951	26.626536917761097
3	19.08406895040117	22.6480352656134	28.366003447689316	29.90189233629611
4	13.148765205880835	15.315526523592654	34.73072694541752	36.80498132510899
5	15.20854479592502	36.04202980384455	33.158406680341365	15.591018719889066
6	35.180301937153445	34.357206488566426	16.312955793338684	14.149535780941443
7	31.00076755333479	29.968382902368234	19.978413621445405	19.052435922851572
8	28.508263608606217	32.81086384107845	18.856514475293896	19.824358075021433
9	26.962459362113332	14.66276802788583	18.558751983705022	39.81602062629582
10	16.894226874363376	26.3706576139736	30.548008819935358	26.187106691727667
11	37.06648813332373	21.399848303156396	21.664088950283265	19.86957461323661
12	24.391624557547058	23.51972638888065	28.304531345201	23.78411770837129
13	29.422262142832935	19.360488763854523	25.041605938379146	26.175643154933397
14	23.691225014064653	19.29407319040486	24.815295560993135	32.199406234537356
15	25.37197324199319	26.857493445237434	21.887229271781557	25.883304040987813
16	25.941735990252617	25.85158284853425	23.207316639185773	24.999364522027363
17	24.23650554640875	26.204673541033085	24.77424154735102	24.78457936520714
18	24.936986092459936	25.19980174447913	25.560474288207608	24.30273787485332
19	25.580585672547564	25.036997379899343	25.04936513597331	24.33305181157978
20	25.78831852769841	24.46390104691242	24.679314775958712	25.06846564943046
21	26.562890442021665	24.25515838763698	24.468024730782947	24.71392643955841
22	26.06563001681626	24.033022564289375	24.84460841829711	25.05673900059725
23	24.79287114221973	24.31017747610247	25.25769353907032	25.639257842607478
24	24.80439052942676	24.713584703961995	25.056986584964015	25.42503818164723
25	25.02519952091264	24.421596878816988	24.971497012480327	25.58170658779005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	271.0
1	271.0
2	319.0
3	367.0
4	367.0
5	367.0
6	844.5
7	1322.0
8	1322.0
9	1322.0
10	1515.0
11	1708.0
12	1708.0
13	1708.0
14	2331.0
15	2954.0
16	2954.0
17	2954.0
18	4895.5
19	6837.0
20	6837.0
21	6837.0
22	10891.0
23	14945.0
24	14945.0
25	14945.0
26	22256.5
27	29568.0
28	29568.0
29	29568.0
30	37173.0
31	44778.0
32	44778.0
33	44778.0
34	56506.5
35	68235.0
36	68235.0
37	68235.0
38	81482.5
39	94730.0
40	94730.0
41	94730.0
42	109152.0
43	123574.0
44	123574.0
45	123574.0
46	138358.5
47	153143.0
48	153143.0
49	153143.0
50	158511.0
51	163879.0
52	163879.0
53	163879.0
54	153886.5
55	143894.0
56	143894.0
57	143894.0
58	133038.5
59	122183.0
60	122183.0
61	122183.0
62	106973.5
63	91764.0
64	91764.0
65	91764.0
66	74706.5
67	57649.0
68	57649.0
69	57649.0
70	43796.5
71	29944.0
72	29944.0
73	29944.0
74	22524.5
75	15105.0
76	15105.0
77	15105.0
78	11963.5
79	8822.0
80	8822.0
81	8822.0
82	6081.5
83	3341.0
84	3341.0
85	3341.0
86	2414.5
87	1488.0
88	1488.0
89	1488.0
90	1013.0
91	538.0
92	538.0
93	538.0
94	337.0
95	136.0
96	136.0
97	136.0
98	314.5
99	493.0
100	493.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.770091091575637E-4
2	8.462613864469547E-5
3	5.923829705128682E-4
4	0.011424528717033887
5	0.03478134298296984
6	0.06431586536996856
7	0.10950622340623593
8	0.11102949390184044
9	0.12076149984598043
10	0.1365865877725385
11	0.14242579133902247
12	0.13531719569286804
13	0.13100126262198858
14	0.11881509865715244
15	0.1362480832179597
16	0.12304640558938722
17	0.12990112281960753
18	0.11619168835916688
19	0.09977421746209594
20	0.0972354333027551
21	0.09435814458883544
22	0.10688281310825037
23	0.08479539092198485
24	0.0953736582525718
25	0.09224249112271805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1181668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.796701017635876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30600155801693	45.8362526095287
2	12.084894478489982	13.969340660059284
3	3.6500240580178844	6.328780475653131
4	1.5778637792068482	3.647812843735009
5	0.849736825383229	2.455599262017478
6	0.51818652777504	1.7969683090308517
7	0.37309150842413336	1.5094420855225887
8	0.2787801059457924	1.2890056346411054
9	0.19565191345370822	1.0177231630871109
>10	1.0430027671579765	10.6566628052482
>50	0.06287803721159375	2.581512660957105
>100	0.054444041663897665	6.459803401139911
>500	0.004855815549811757	1.9521341950529008
>1k	5.885837030074857E-4	0.4989618943267129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2114	0.1788996570948862	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1603	0.13565570024744683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.462613864469547E-5
7	0.0	0.0	0.0	0.0	8.462613864469547E-5
8	0.0	0.0	0.0	0.0	8.462613864469547E-5
9	0.0	0.0	0.0	0.0	8.462613864469547E-5
10	0.0	0.0	0.0	0.0	8.462613864469547E-5
11	0.0	0.0	0.0	0.0	8.462613864469547E-5
12	0.0	0.0	0.0	0.0	2.538784159340864E-4
13	0.0	0.0	0.0	0.0	2.538784159340864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	465	0.0	14.294413	1
TAGGACA	95	4.8967195E-9	14.0	4
CTACACG	70	7.258999E-6	13.571428	4
GCGTTAT	80	2.0087227E-6	13.056414	1
CCGTCGT	155	0.0	12.87315	9
GTATCAA	1345	0.0	12.707831	1
GTGTAGG	105	2.0045263E-8	12.660766	1
GTCCTAA	60	4.1068436E-4	12.660765	1
CGTTATT	85	3.9522583E-6	12.290993	2
CGTCGTA	155	1.8189894E-12	12.259624	10
CGGACAT	125	1.4078978E-9	12.160516	5
CACGCCC	120	1.0013537E-8	11.873993	16
GGTTTAT	80	2.878688E-5	11.869467	1
TTAGACA	65	8.0209115E-4	11.692307	4
ACGCCCC	125	1.8317223E-8	11.3990345	17
ACTCTAA	160	4.5474735E-11	11.282684	10
GTCGGCG	110	4.9555456E-7	11.228701	12
AGAACCG	110	4.9609844E-7	11.227749	5
AAGACGG	280	0.0	11.196903	5
AACCGCG	85	5.310928E-5	11.178366	7
>>END_MODULE