FastQCFastQC Report
Thu 2 Feb 2017
SRR4062278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181668
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.031.191031
GTATCAA15200.025.0474471
GTGTATA2000.018.7059561
GTTATAT951.592686E-518.5322151
TATACAG1852.1827873E-1117.8374775
CGAATGC2500.017.6003943
CGCAATA2650.017.4343536
TACGAAT2400.017.41705141
CTAGCGG2550.017.25455529
ACCATAC4250.017.0877911
GCATTCG4400.016.99965722
TCAACGC22200.016.9456024
CAATACG2600.016.92345238
CAACGCA22450.016.75695
ATTCGTA4600.016.73879224
ATCAACG23250.016.3695623
TCTAGCG2700.016.29596928
AACGCAG23250.016.274946
CCTATTC3000.016.1330073
ATACGAA2600.016.07727840
Produced by FastQC (version 0.11.5)