##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181668
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303323776221408	32.0	32.0	32.0	32.0	32.0
2	31.466609064474962	32.0	32.0	32.0	32.0	32.0
3	31.58144504209304	32.0	32.0	32.0	32.0	32.0
4	31.65752478699601	32.0	32.0	32.0	32.0	32.0
5	31.631911839873805	32.0	32.0	32.0	32.0	32.0
6	35.27768967256454	36.0	36.0	36.0	36.0	36.0
7	35.28041040292197	36.0	36.0	36.0	36.0	36.0
8	35.233805095847565	36.0	36.0	36.0	36.0	36.0
9	35.318812898377544	36.0	36.0	36.0	36.0	36.0
10	35.20114871520596	36.0	36.0	36.0	36.0	36.0
11	35.316849571960994	36.0	36.0	36.0	36.0	36.0
12	35.2385923965107	36.0	36.0	36.0	36.0	36.0
13	35.27301407840442	36.0	36.0	36.0	36.0	36.0
14	35.23928463832481	36.0	36.0	36.0	36.0	36.0
15	35.22047563274964	36.0	36.0	36.0	36.0	36.0
16	35.227409898550185	36.0	36.0	36.0	36.0	36.0
17	35.20967141362887	36.0	36.0	36.0	36.0	36.0
18	35.20529708852233	36.0	36.0	36.0	36.0	36.0
19	35.20328721772952	36.0	36.0	36.0	36.0	36.0
20	35.19793376819885	36.0	36.0	36.0	36.0	36.0
21	35.191558034913356	36.0	36.0	36.0	36.0	36.0
22	35.1737535416039	36.0	36.0	36.0	36.0	36.0
23	35.14157022107732	36.0	36.0	36.0	36.0	36.0
24	35.124736389578125	36.0	36.0	36.0	36.0	36.0
25	35.115986893103646	36.0	36.0	36.0	36.0	36.0
26	35.080254352322314	36.0	36.0	36.0	36.0	36.0
27	35.067709373529624	36.0	36.0	36.0	36.0	36.0
28	35.05176580900896	36.0	36.0	36.0	36.0	36.0
29	35.028330292434084	36.0	36.0	36.0	36.0	36.0
30	35.00158927888375	36.0	36.0	36.0	36.0	36.0
31	35.01036754824536	36.0	36.0	36.0	36.0	36.0
32	34.98538760464022	36.0	36.0	36.0	36.0	36.0
33	34.96215603705948	36.0	36.0	36.0	36.0	36.0
34	34.95284970059272	36.0	36.0	36.0	36.0	36.0
35	34.94217749824824	36.0	36.0	36.0	36.0	36.0
36	34.91499473625418	36.0	36.0	36.0	36.0	36.0
37	34.91479924987391	36.0	36.0	36.0	32.0	36.0
38	34.89497303811223	36.0	36.0	36.0	32.0	36.0
39	34.884167972730076	36.0	36.0	36.0	32.0	36.0
40	34.86077984679284	36.0	36.0	36.0	32.0	36.0
41	34.84048480622307	36.0	36.0	36.0	32.0	36.0
42	34.814625597037406	36.0	36.0	36.0	32.0	36.0
43	34.81021403642986	36.0	36.0	36.0	32.0	36.0
44	34.765039757359936	36.0	36.0	36.0	32.0	36.0
45	34.74848688464103	36.0	36.0	36.0	32.0	36.0
46	34.742246553177374	36.0	36.0	36.0	32.0	36.0
47	34.70450244908045	36.0	36.0	36.0	32.0	36.0
48	34.68103562083428	36.0	36.0	36.0	32.0	36.0
49	34.670616450644346	36.0	36.0	36.0	32.0	36.0
50	34.258633558664535	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	8.0
21	37.0
22	83.0
23	200.0
24	517.0
25	1191.0
26	2581.0
27	5027.0
28	8769.0
29	14237.0
30	21518.0
31	31748.0
32	47841.0
33	81469.0
34	188159.0
35	778279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65888861142032	17.894211112192767	12.464499307974588	25.98240096841232
2	16.208263195444147	19.671191882877554	36.971955846347456	27.148589075330843
3	18.47417121250844	23.01877180184587	28.626447744117208	29.88060924152849
4	12.93029254434625	15.79277410641069	34.8478886183671	36.42904473087596
5	14.950053652971901	36.39787148336081	32.916267513379395	15.735807350287898
6	35.31403966941589	34.55646519405653	16.21676514345001	13.91272999307757
7	31.157905604619913	30.066397668380628	19.842629232576325	18.933067494423135
8	28.5919564547741	33.03085130510431	18.689598093542347	19.687594146579244
9	27.11049127166006	14.39152113791691	18.551488235274206	39.94649935514882
10	16.884621471192816	26.470655438461087	30.55395470974479	26.090768380601304
11	37.23533175138872	21.241076173679918	21.66471462373526	19.858877451196104
12	24.464655046933657	23.481976324991454	28.328176780618584	23.725191847456305
13	29.456835591722886	19.277834383261627	25.11712257588426	26.148207449131228
14	23.698873118337808	19.112221029933956	24.870098877180393	32.31880697454784
15	25.353144876564315	26.939461845459128	21.862485909747917	25.844907368228636
16	26.037140908313884	25.704981627572014	23.264012890318888	24.993864573795214
17	24.346263078969727	26.095316112478294	24.723441778909137	24.834979029642845
18	25.165973154873583	25.001798306968038	25.59071210417148	24.241516433986902
19	25.767897582061966	24.8460650538053	25.16722124996192	24.218816114170817
20	26.052285501994643	24.24856093007669	24.721960350894413	24.977193217034255
21	26.696838705964787	24.12174993314535	24.592440516287148	24.588970844602713
22	26.214385089551378	23.88234258691951	24.912665824918676	24.990606498610436
23	24.831763236374346	24.0642887850056	25.400197009650764	25.703750968969285
24	25.0	24.57441082683962	25.218930043938336	25.206659129222043
25	25.269724888653062	24.164845087490807	25.152179082601105	25.413250941255022
26	25.01277859019146	25.015655888645167	25.298985159910092	24.67258036125328
27	25.365544461952744	24.603566840602472	24.831296936010467	25.19959176143432
28	25.12273123868634	24.36201335931966	25.424005646356314	25.091249755637683
29	24.932404585740606	24.717961043038496	25.299091196051666	25.05054317516923
30	24.934942719974	24.835251694451237	25.333368312640538	24.896437272934225
31	25.523353183955777	24.427520968401208	24.707212486851112	25.3419133607919
32	25.34669536083652	24.513374016317762	24.585899292265015	25.554031330580703
33	24.807665780630245	24.401206092748634	25.08667839585941	25.704449730761713
34	25.57844326174214	24.42532261736214	25.168174005617505	24.828060115278213
35	25.873238185103226	24.35918809476459	25.162122936703717	24.605450783428466
36	24.911692474861763	24.867856060849682	24.869040828795956	25.3514106354926
37	25.79968417150875	24.55896565322844	24.7928750717215	24.848475103541308
38	25.171135353699476	24.349668730699936	25.148878660400943	25.330317255199645
39	25.648346942730516	24.38757712673453	24.66650700413571	25.29756892639924
40	25.80403294326325	24.489281253279263	24.896502232437538	24.810183571019948
41	24.901620948264043	24.950789317346704	25.21347196387437	24.934117770514888
42	25.906533165992023	24.794582339285913	24.716130522834387	24.582753971887676
43	25.550641406473474	24.056339506204182	25.019591713762946	25.373427373559394
44	25.106485254553284	24.67030404893625	24.844471864450384	25.378738832060083
45	25.26501078999704	24.893073245038718	24.74565226589938	25.09626369906487
46	25.14562326248577	24.580986675355117	24.906803650842683	25.366586411316426
47	25.27522745563652	24.613192130724684	25.06772258324948	25.04385783038931
48	25.870587398951944	25.0538245927611	24.23020676768087	24.845381240606084
49	25.257949202287016	25.189230451546514	24.40353478421297	25.149285561953512
50	25.35245237799042	25.22009496897979	24.42265004244069	25.0048026105891
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	81.5
2	52.0
3	132.0
4	212.0
5	216.0
6	220.0
7	190.0
8	160.0
9	208.0
10	256.0
11	408.0
12	560.0
13	1084.0
14	1608.0
15	1998.5
16	2389.0
17	2342.5
18	2296.0
19	2130.5
20	1965.0
21	2260.5
22	2556.0
23	3188.0
24	3820.0
25	4869.0
26	5918.0
27	7456.0
28	8994.0
29	10729.5
30	12465.0
31	14367.0
32	16269.0
33	18832.0
34	21395.0
35	25339.5
36	29284.0
37	34798.5
38	40313.0
39	44802.0
40	49291.0
41	54295.0
42	59299.0
43	62217.5
44	65136.0
45	71219.5
46	77303.0
47	82287.5
48	87272.0
49	90752.5
50	94233.0
51	92982.5
52	91732.0
53	91140.5
54	90549.0
55	90035.0
56	89521.0
57	86605.5
58	83690.0
59	76736.5
60	69783.0
61	61100.0
62	52417.0
63	45513.0
64	38609.0
65	32418.5
66	26228.0
67	22568.5
68	18909.0
69	16454.5
70	14000.0
71	11051.5
72	8103.0
73	7090.5
74	6078.0
75	4555.5
76	3033.0
77	2625.5
78	2218.0
79	1854.5
80	1491.0
81	1174.0
82	857.0
83	714.5
84	572.0
85	435.0
86	298.0
87	199.5
88	101.0
89	72.0
90	43.0
91	28.5
92	14.0
93	15.0
94	16.0
95	12.0
96	8.0
97	11.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0298730269415775
2	0.0056699512891945966
3	1.6925227728939094E-4
4	8.462613864469547E-5
5	0.0
6	1.6925227728939094E-4
7	0.0
8	0.0
9	0.0
10	8.462613864469547E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.077568318681728E-4
17	0.0
18	8.462613864469547E-5
19	0.0
20	1.6925227728939094E-4
21	0.0
22	0.0
23	0.0
24	0.0010155136637363456
25	8.462613864469547E-5
26	3.385045545787819E-4
27	5.077568318681728E-4
28	0.0017771489115386047
29	4.2313069322347734E-4
30	0.001438644356959823
31	7.616352478022592E-4
32	9.3088752509165E-4
33	8.462613864469547E-5
34	4.2313069322347734E-4
35	2.538784159340864E-4
36	1.6925227728939094E-4
37	0.0011847659410257365
38	8.462613864469547E-5
39	7.616352478022592E-4
40	0.0
41	0.0011847659410257365
42	0.004315933070879468
43	0.0038928023776559914
44	0.004146680793590078
45	0.0015232704956045183
46	0.0019464011888279957
47	7.616352478022592E-4
48	0.004400559209524164
49	0.0033850455457878187
50	0.0016078966342492138
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1181668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.95570964438163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79486848092469	46.45407387628634
2	12.672717023289213	14.942580504609019
3	3.674504430854967	6.498990489374375
4	1.5441491629410287	3.641456387918649
5	0.850069508326484	2.5058225555219216
6	0.533118590052655	1.885823088069987
7	0.3657270723199805	1.509318935935057
8	0.26815190404704653	1.2647268636468605
9	0.20784562656004824	1.10283177692959
>10	0.9777959137315675	10.111813471818772
>50	0.05894706886198112	2.424344720951525
>100	0.04763081306813798	5.740621688652911
>500	0.004041398084474843	1.6531231641365982
>1k	4.330069376223045E-4	0.26447247614848135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.462613864469547E-5
7	0.0	0.0	0.0	0.0	8.462613864469547E-5
8	0.0	0.0	0.0	0.0	1.6925227728939094E-4
9	0.0	0.0	0.0	0.0	1.6925227728939094E-4
10	0.0	0.0	0.0	0.0	1.6925227728939094E-4
11	0.0	0.0	0.0	0.0	1.6925227728939094E-4
12	0.0	0.0	0.0	0.0	3.385045545787819E-4
13	0.0	0.0	0.0	0.0	3.385045545787819E-4
14	0.0	0.0	0.0	0.0	3.385045545787819E-4
15	0.0	0.0	0.0	0.0	4.2313069322347734E-4
16	0.0	0.0	0.0	0.0	4.2313069322347734E-4
17	0.0	0.0	0.0	0.0	4.2313069322347734E-4
18	0.0	0.0	0.0	0.0	5.077568318681728E-4
19	0.0	0.0	0.0	0.0	5.077568318681728E-4
20	0.0	0.0	0.0	0.0	5.923829705128682E-4
21	0.0	0.0	0.0	0.0	7.616352478022592E-4
22	0.0	0.0	0.0	8.462613864469547E-5	7.616352478022592E-4
23	0.0	0.0	0.0	8.462613864469547E-5	8.462613864469547E-4
24	0.0	0.0	0.0	3.385045545787819E-4	8.462613864469547E-4
25	0.0	0.0	0.0	5.923829705128682E-4	9.308875250916501E-4
26	0.0	0.0	0.0	7.616352478022592E-4	0.0010155136637363456
27	0.0	0.0	0.0	7.616352478022592E-4	0.0010155136637363456
28	0.0	0.0	0.0	0.0014386443569598228	0.0010155136637363456
29	0.0	0.0	0.0	0.0021156534661173868	0.0010155136637363456
30	0.0	0.0	0.0	0.004485185348168859	0.0010155136637363456
31	0.0	0.0	0.0	0.007700978616667287	0.0011001398023810411
32	0.0	0.0	0.0	0.012440042380770233	0.0011001398023810411
33	0.0	0.0	0.0	0.014894200401466402	0.0011847659410257365
34	0.0	0.0	0.0	0.018533124363188307	0.0014386443569598228
35	0.0	0.0	0.0	0.023356814265935947	0.0014386443569598228
36	0.0	0.0	0.0	0.03038078377344567	0.0014386443569598228
37	0.0	0.0	0.0	0.04036666813351974	0.0014386443569598228
38	0.0	0.0	0.0	0.05559937308956492	0.0014386443569598228
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	635	0.0	31.19103	1
GTATCAA	1520	0.0	25.047447	1
GTGTATA	200	0.0	18.705956	1
GTTATAT	95	1.592686E-5	18.532215	1
TATACAG	185	2.1827873E-11	17.837477	5
CGAATGC	250	0.0	17.60039	43
CGCAATA	265	0.0	17.43435	36
TACGAAT	240	0.0	17.417051	41
CTAGCGG	255	0.0	17.254555	29
ACCATAC	425	0.0	17.087791	1
GCATTCG	440	0.0	16.999657	22
TCAACGC	2220	0.0	16.945602	4
CAATACG	260	0.0	16.923452	38
CAACGCA	2245	0.0	16.7569	5
ATTCGTA	460	0.0	16.738792	24
ATCAACG	2325	0.0	16.369562	3
TCTAGCG	270	0.0	16.295969	28
AACGCAG	2325	0.0	16.27494	6
CCTATTC	300	0.0	16.133007	3
ATACGAA	260	0.0	16.077278	40
>>END_MODULE