FastQCFastQC Report
Thu 2 Feb 2017
SRR4062277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257111
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC21490.1709475137835879No Hit
GGGTAGGCACACGCTGAGCCAGTCA17660.14048083263928166No Hit
GAATAGGACCGCGGTTCTATTTTGT16960.1349125097147348No Hit
GATTAAGAGGGACGGCCGGGGGCAT16350.130060114023344No Hit
TCGTAGTTCCGACCATAAACGATGC16110.12815097473492792No Hit
GAACTACGACGGTATCTGATCGTCT16000.12727595256107058No Hit
GAATAACGCCGCCGCATCGCCAGTC15650.12449179109879716No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15510.12337812651388778No Hit
ATCAGATACCGTCGTAGTTCCGACC15460.12298038916213445No Hit
GTATCAACGCAGAGTACTTTTTTTT15420.12266219928073177No Hit
GTATCTGATCGTCTTCGAACCTCCG14830.1179688985300423No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14610.11621885418232757No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT13650.10858229702866333No Hit
ACCATACTCCCCCCGGAACCCAAAG13590.10810501220655931No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC13460.10707089509200063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG400.005276228314.25086513
TCGACGC400.00527762314.250298514
ATCGCCA2200.014.24916416
GGTATCA4400.014.0293561
TCGCCAG2100.014.0218717
CGCATCG2150.013.69850713
CGTTATT1257.2759576E-1213.6775642
CGCGTAA1900.013.50189610
GCGTAAC1900.013.50189611
TCGCGTA1900.013.5008219
CACGCGC500.001497681313.30186912
CAAGACG4000.012.825274
CAGAACG604.0903297E-412.6669324
CGTTCCA604.093079E-412.66592418
GTAATAC751.4801521E-512.6649143
GCGTTAT1352.7284841E-1112.6623941
CGGACCA4000.012.5882659
CGACCAT2650.012.54893210
CGCCAGT2350.012.53117818
GTATCAA11550.012.4979471
Produced by FastQC (version 0.11.5)