##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062277_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1257111
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251978544456296	32.0	32.0	32.0	32.0	32.0
2	30.916265150810073	32.0	32.0	32.0	32.0	32.0
3	30.829583863318355	32.0	32.0	32.0	32.0	32.0
4	30.94573669310029	32.0	32.0	32.0	32.0	32.0
5	30.860793517835734	32.0	32.0	32.0	32.0	32.0
6	34.4197982517057	36.0	36.0	36.0	32.0	36.0
7	34.18733747457464	36.0	36.0	36.0	32.0	36.0
8	34.102831810397014	36.0	36.0	36.0	32.0	36.0
9	34.474549980073355	36.0	36.0	36.0	32.0	36.0
10	34.03640410433128	36.0	36.0	36.0	32.0	36.0
11	34.18675280066756	36.0	36.0	36.0	32.0	36.0
12	34.133277013724324	36.0	36.0	36.0	32.0	36.0
13	34.162354000561606	36.0	36.0	36.0	32.0	36.0
14	34.12357142686684	36.0	36.0	36.0	32.0	36.0
15	34.27244929047634	36.0	36.0	36.0	32.0	36.0
16	34.27235701541073	36.0	36.0	36.0	32.0	36.0
17	34.04971716896917	36.0	36.0	36.0	32.0	36.0
18	34.21827905411694	36.0	36.0	36.0	32.0	36.0
19	34.213892806601805	36.0	36.0	36.0	32.0	36.0
20	34.026337371958405	36.0	36.0	36.0	32.0	36.0
21	33.97165723631406	36.0	36.0	36.0	32.0	36.0
22	34.12570170812283	36.0	36.0	36.0	32.0	36.0
23	33.947021384746456	36.0	36.0	36.0	32.0	36.0
24	33.91622139970138	36.0	36.0	36.0	32.0	36.0
25	33.612810642815155	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	16.0
5	84.0
6	241.0
7	56.0
8	218.0
9	178.0
10	92.0
11	25.0
12	47.0
13	42.0
14	185.0
15	359.0
16	612.0
17	818.0
18	1116.0
19	1343.0
20	1841.0
21	2450.0
22	3419.0
23	4704.0
24	6514.0
25	9022.0
26	12914.0
27	16724.0
28	23095.0
29	31522.0
30	42799.0
31	61286.0
32	92167.0
33	138047.0
34	310594.0
35	494579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82395167282105	17.750803835500495	11.5643330909762	25.860911400702253
2	16.69993087302831	19.486964912294656	37.631959729760474	26.181144484916558
3	19.300407365188054	22.868690950542636	28.303488258320918	29.52741342594839
4	12.550407596481472	15.433694271214945	35.80659712029327	36.209301012010314
5	14.784164847782685	36.45926747580812	33.54112242713788	15.215445249271319
6	34.66475962613728	35.412771600059536	16.42864476753765	13.493824006265537
7	30.489141080747935	30.20048828109448	20.3427363807637	18.967634257393893
8	27.77276553117383	33.50801433015903	19.24339053664837	19.47582960201877
9	26.583986168417518	14.526028588562188	18.926125110199518	39.963860132820784
10	16.074271782409674	26.70528085690368	31.036474001929115	26.183973358757527
11	37.5196748786052	21.383088207180453	21.846045269744977	19.25119164446937
12	24.06722555208188	23.890718652302915	28.697743881797727	23.344311913817478
13	28.870663797236872	19.473546713987833	25.380674677069674	26.27511481170562
14	22.966136046718127	20.178529761463913	24.83736176048279	32.01797243133517
15	24.83370994681745	27.770817220727896	22.01161780249417	25.383855029960483
16	25.491578067136544	26.240672161828538	23.809580695257438	24.458169075777487
17	23.571515041462384	26.576462492712622	25.33298927361986	24.519033192205136
18	24.5794760920721	25.331135452377257	26.254086187194158	23.835302268356486
19	25.215356453846162	25.318784879226016	25.166946536501317	24.29891213042651
20	25.360712619408176	24.842792833678082	24.911424171925482	24.885070374988256
21	26.328758965082354	24.74649358590555	24.380246882125885	24.544500566886203
22	25.450772548182737	24.791853128254786	25.391131382560257	24.366242941002227
23	23.87199549084795	24.80240615486517	25.66323703618786	25.662361318099023
24	24.2370247228512	25.387021060442482	25.589090480603566	24.786863736102752
25	24.298339198420408	24.89705577936657	25.495613127179507	25.308991895033518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	136.0
1	136.0
2	235.5
3	335.0
4	335.0
5	335.0
6	764.5
7	1194.0
8	1194.0
9	1194.0
10	1566.5
11	1939.0
12	1939.0
13	1939.0
14	2730.5
15	3522.0
16	3522.0
17	3522.0
18	5992.5
19	8463.0
20	8463.0
21	8463.0
22	13736.0
23	19009.0
24	19009.0
25	19009.0
26	28825.5
27	38642.0
28	38642.0
29	38642.0
30	48457.0
31	58272.0
32	58272.0
33	58272.0
34	71429.5
35	84587.0
36	84587.0
37	84587.0
38	95379.5
39	106172.0
40	106172.0
41	106172.0
42	119779.5
43	133387.0
44	133387.0
45	133387.0
46	146707.5
47	160028.0
48	160028.0
49	160028.0
50	162175.5
51	164323.0
52	164323.0
53	164323.0
54	152117.5
55	139912.0
56	139912.0
57	139912.0
58	129135.5
59	118359.0
60	118359.0
61	118359.0
62	105575.5
63	92792.0
64	92792.0
65	92792.0
66	76556.5
67	60321.0
68	60321.0
69	60321.0
70	45568.5
71	30816.0
72	30816.0
73	30816.0
74	23692.0
75	16568.0
76	16568.0
77	16568.0
78	13780.0
79	10992.0
80	10992.0
81	10992.0
82	7532.5
83	4073.0
84	4073.0
85	4073.0
86	3072.0
87	2071.0
88	2071.0
89	2071.0
90	1320.0
91	569.0
92	569.0
93	569.0
94	346.5
95	124.0
96	124.0
97	124.0
98	314.5
99	505.0
100	505.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010341171145586984
2	3.1818988140267646E-4
3	6.363797628053529E-4
4	0.008591126797872264
5	0.030864418496059615
6	0.056796893830377754
7	0.10158211963780446
8	0.10325261651516851
9	0.1140710724828595
10	0.12838961714597996
11	0.1354693420071895
12	0.13013966149369466
13	0.1254463607430052
14	0.11216193319444345
15	0.12918509184948662
16	0.11621885418232757
17	0.1205144175812637
18	0.10786636979550732
19	0.09307054031028286
20	0.08949090414450275
21	0.08964999908520409
22	0.10078664493429776
23	0.07946792288031845
24	0.08805904967819071
25	0.08678629015258
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1257111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.41525469332107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41288602701236	39.28787996930555
2	13.901511143893197	14.294994721706198
3	4.49221841980596	6.929056625770552
4	1.816740100031567	3.736326198187704
5	0.9364998363852505	2.407518880400058
6	0.5318103632889171	1.6405899166228362
7	0.34997624313567666	1.2595882374202731
8	0.23537986213571857	0.9681692449109415
9	0.17480446649008385	0.8088854549505994
>10	0.9552653149234533	9.12411591852487
>50	0.09128953562421609	3.271330100419668
>100	0.08719039062902796	9.748585585325467
>500	0.01054972227771645	3.7504921204284245
>1k	0.003878574366807518	2.772467026026857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2149	0.1709475137835879	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1766	0.14048083263928166	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1696	0.1349125097147348	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1635	0.130060114023344	No Hit
TCGTAGTTCCGACCATAAACGATGC	1611	0.12815097473492792	No Hit
GAACTACGACGGTATCTGATCGTCT	1600	0.12727595256107058	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1565	0.12449179109879716	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1551	0.12337812651388778	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1546	0.12298038916213445	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1542	0.12266219928073177	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1483	0.1179688985300423	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1461	0.11621885418232757	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1365	0.10858229702866333	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1359	0.10810501220655931	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1346	0.10707089509200063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	7.954747035066911E-5	0.0	0.0	0.0
8	0.0	7.954747035066911E-5	0.0	0.0	0.0
9	0.0	7.954747035066911E-5	0.0	7.954747035066911E-5	0.0
10	0.0	7.954747035066911E-5	0.0	7.954747035066911E-5	0.0
11	0.0	7.954747035066911E-5	0.0	7.954747035066911E-5	0.0
12	0.0	7.954747035066911E-5	0.0	7.954747035066911E-5	2.3864241105200733E-4
13	0.0	7.954747035066911E-5	0.0	7.954747035066911E-5	3.1818988140267646E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	40	0.0052762283	14.250865	13
TCGACGC	40	0.005277623	14.2502985	14
ATCGCCA	220	0.0	14.249164	16
GGTATCA	440	0.0	14.029356	1
TCGCCAG	210	0.0	14.02187	17
CGCATCG	215	0.0	13.698507	13
CGTTATT	125	7.2759576E-12	13.677564	2
CGCGTAA	190	0.0	13.501896	10
GCGTAAC	190	0.0	13.501896	11
TCGCGTA	190	0.0	13.500821	9
CACGCGC	50	0.0014976813	13.301869	12
CAAGACG	400	0.0	12.82527	4
CAGAACG	60	4.0903297E-4	12.666932	4
CGTTCCA	60	4.093079E-4	12.665924	18
GTAATAC	75	1.4801521E-5	12.664914	3
GCGTTAT	135	2.7284841E-11	12.662394	1
CGGACCA	400	0.0	12.588265	9
CGACCAT	265	0.0	12.548932	10
CGCCAGT	235	0.0	12.531178	18
GTATCAA	1155	0.0	12.497947	1
>>END_MODULE