FastQCFastQC Report
Thu 2 Feb 2017
SRR4062277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257111
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21610.17190208342779595No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18070.1437422789236591No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17550.13960581046542428No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17200.13682164900315086No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16370.13021920896404535No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16110.12815097473492792No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15900.1264804778575639No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15720.12504862339125186No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG15170.12067351252196504No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14870.11828708841144497No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14690.11685523394513292No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC14180.1127983129572488No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT12990.10333216398551918No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG12640.10054800252324576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.028.4564041
GTATCAA14100.025.4398921
GGCGTTA4800.020.16761642
TGCGTAG708.125346E-418.85653113
TAGTACG600.007413652318.3327372
CGCAATA3050.018.03363636
CCGTCGT5200.017.7686529
CGTCGTA5200.017.76865210
TAGGACG2600.017.7686524
CGGCGTT5700.017.75522241
GATATAC3500.017.6050281
CGTTTAG1002.4838368E-517.59942613
ACGGTAT6150.017.5278859
TACGACG5900.017.5248535
ACCGTCG5400.017.5179488
ATACCGT5150.017.5139926
CGACGGT5950.017.3775867
TACCGTC5200.017.3455897
GACGGTA6100.017.3109138
CGCGGGA3500.016.97087744
Produced by FastQC (version 0.11.5)