##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1257111
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140266054469336	32.0	32.0	32.0	32.0	32.0
2	31.207229910485232	32.0	32.0	32.0	32.0	32.0
3	31.434461236915435	32.0	32.0	32.0	32.0	32.0
4	31.545643145275157	32.0	32.0	32.0	32.0	32.0
5	31.47731107276923	32.0	32.0	32.0	32.0	32.0
6	35.1095201617041	36.0	36.0	36.0	36.0	36.0
7	35.1156222481547	36.0	36.0	36.0	36.0	36.0
8	35.043774972933974	36.0	36.0	36.0	36.0	36.0
9	35.1786850962246	36.0	36.0	36.0	36.0	36.0
10	35.00443636242146	36.0	36.0	36.0	36.0	36.0
11	35.1846288832092	36.0	36.0	36.0	36.0	36.0
12	35.06851344073833	36.0	36.0	36.0	36.0	36.0
13	35.13146333140033	36.0	36.0	36.0	36.0	36.0
14	35.08691356610514	36.0	36.0	36.0	36.0	36.0
15	35.042814039492136	36.0	36.0	36.0	36.0	36.0
16	35.06613178947603	36.0	36.0	36.0	36.0	36.0
17	35.0391524694319	36.0	36.0	36.0	36.0	36.0
18	35.0350024779037	36.0	36.0	36.0	36.0	36.0
19	35.03637228534314	36.0	36.0	36.0	36.0	36.0
20	35.038852575468674	36.0	36.0	36.0	36.0	36.0
21	35.03020417449215	36.0	36.0	36.0	36.0	36.0
22	35.007348595310994	36.0	36.0	36.0	36.0	36.0
23	34.96163425504987	36.0	36.0	36.0	32.0	36.0
24	34.94485053428058	36.0	36.0	36.0	32.0	36.0
25	34.92428989961905	36.0	36.0	36.0	32.0	36.0
26	34.877924065575755	36.0	36.0	36.0	32.0	36.0
27	34.87042751196991	36.0	36.0	36.0	32.0	36.0
28	34.83471467515597	36.0	36.0	36.0	32.0	36.0
29	34.810148825362276	36.0	36.0	36.0	32.0	36.0
30	34.80270636403627	36.0	36.0	36.0	32.0	36.0
31	34.79786430951602	36.0	36.0	36.0	32.0	36.0
32	34.76899016872814	36.0	36.0	36.0	32.0	36.0
33	34.74357713837521	36.0	36.0	36.0	32.0	36.0
34	34.739326917034376	36.0	36.0	36.0	32.0	36.0
35	34.72423119358593	36.0	36.0	36.0	32.0	36.0
36	34.681644659858996	36.0	36.0	36.0	32.0	36.0
37	34.6751360858349	36.0	36.0	36.0	32.0	36.0
38	34.653892933877756	36.0	36.0	36.0	32.0	36.0
39	34.63272773844155	36.0	36.0	36.0	32.0	36.0
40	34.62049970129925	36.0	36.0	36.0	32.0	36.0
41	34.581425983863	36.0	36.0	36.0	32.0	36.0
42	34.55049792739066	36.0	36.0	36.0	32.0	36.0
43	34.55747662696452	36.0	36.0	36.0	32.0	36.0
44	34.483925444928886	36.0	36.0	36.0	32.0	36.0
45	34.483055991077954	36.0	36.0	36.0	32.0	36.0
46	34.456602479812844	36.0	36.0	36.0	32.0	36.0
47	34.44137948041183	36.0	36.0	36.0	32.0	36.0
48	34.41597042743242	36.0	36.0	36.0	32.0	36.0
49	34.39733404607867	36.0	36.0	36.0	32.0	36.0
50	33.92475366137119	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	5.0
21	24.0
22	59.0
23	210.0
24	600.0
25	1307.0
26	2936.0
27	5711.0
28	10358.0
29	17124.0
30	26879.0
31	41482.0
32	66527.0
33	116989.0
34	261065.0
35	705831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7172429597189	17.837978051637215	11.797483704231809	25.64729528441208
2	16.206940990601836	19.97949173225653	37.56568930213022	26.247877975011413
3	18.908016580927477	23.282027703290172	28.644101098792706	29.165854616989645
4	12.470179642052292	15.937892517049013	35.97613894079361	35.61578890010508
5	14.737612460325666	36.687004319431075	33.11730874784227	15.458074472400984
6	34.82652246266829	35.69613748381205	16.292156282515105	13.18518377100456
7	30.737222090968896	30.129956702311887	20.1449991289552	18.987822077764015
8	28.073018213984284	33.50443994205762	19.02258432230726	19.399957521650833
9	26.827225280822457	14.346386277743175	18.757452603628476	40.06893583780589
10	16.198967314739907	26.642436507197854	30.93259067815014	26.226005499912098
11	37.91868816675695	21.214912605171698	21.604456567478923	19.26194266059242
12	24.29411563497575	23.71150996212745	28.77232002583702	23.22205437705978
13	29.139192959094306	19.261783565651722	25.269844906297056	26.32917856895692
14	23.334057215313525	19.93841434845451	24.857152630117785	31.870375806114176
15	25.07654455334493	27.522231529276254	21.933703547260343	25.467520370118468
16	25.76266683212659	26.09462080244881	23.736419160895455	24.40629320452915
17	23.92215166361602	26.509114946890133	25.04878248619255	24.5199509033013
18	24.8822896307486	25.211059325707915	26.19760705299691	23.70904399054658
19	25.538158523789868	25.136602893459685	25.150444153300704	24.174794429449747
20	25.62586259699866	24.667629195651912	24.790848815333643	24.915659392015783
21	26.650492001495497	24.522197739259095	24.419263230743532	24.40804702850188
22	25.69367382832542	24.765513944273813	25.193320239819712	24.347491987581048
23	24.170021581228706	24.683261859931225	25.66734361563935	25.47937294320072
24	24.576883302839867	25.243656033728424	25.36838755866677	24.811073104764937
25	24.721683288110597	24.781025700992195	25.32735772736059	25.169933283536615
26	24.204523398148766	26.047960875278815	25.82729566592528	23.920220060647136
27	25.116398206357932	24.91331286835568	25.321313141999845	24.648975783286545
28	24.314793616752166	25.102954968685935	25.749043634451592	24.8332077801103
29	24.22600126639875	25.238404337574817	25.685780378455945	24.849814017570488
30	24.145276878664674	25.5507305288429	25.926356074243717	24.377636518248703
31	25.06226588147885	25.216429124284367	24.893386333245566	24.82791866099121
32	24.958694778876865	25.06656166350464	24.672561162353183	25.30218239526531
33	24.306783018192522	24.85247909888554	25.522189784505734	25.318548098416212
34	25.14459856447493	25.226453562601574	25.63016018601528	23.998787686908212
35	25.74365248435693	24.612403409099947	25.648751974773802	23.99519213176932
36	23.976161138232467	25.651515742096738	25.310176460714963	25.062146658955843
37	25.280170425076285	25.37069681455295	25.12019804437543	24.22893471599533
38	24.646272286218164	24.884437412448065	25.55207933110123	24.917210970232542
39	25.413151646043886	24.79856495678563	24.830543192493863	24.957740204676618
40	25.648968149988345	25.217184480924914	25.28973177388473	23.844115595202016
41	24.190457553462377	25.62304162202022	25.990633991435836	24.195866833081563
42	25.77729234019805	25.63123679454217	25.114951123895835	23.476519741363948
43	25.006582794048036	24.60803538400958	25.570754097839014	24.81462772410337
44	24.372149115480017	25.472886865128803	25.066862042247585	25.0881019771436
45	24.571072192241118	25.764465176662256	25.24055595153243	24.4239066795642
46	24.400321377148813	25.222537765792424	25.109101178117715	25.268039678941044
47	24.776668878102175	25.023108705578384	25.65845889991425	24.541763516405194
48	25.68303402740489	25.53610564206591	24.408169759163137	24.372690571366068
49	24.621937113882645	25.817180641271026	24.81564213812163	24.745240106724705
50	25.041424938329943	25.74924846451297	24.618067239257556	24.591259357899535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	25.5
2	17.0
3	58.0
4	99.0
5	116.0
6	133.0
7	109.0
8	85.0
9	124.5
10	164.0
11	254.0
12	344.0
13	626.0
14	908.0
15	1215.0
16	1522.0
17	1684.5
18	1847.0
19	2012.5
20	2178.0
21	2865.5
22	3553.0
23	4356.0
24	5159.0
25	6813.5
26	8468.0
27	11174.0
28	13880.0
29	16270.5
30	18661.0
31	21152.0
32	23643.0
33	26892.5
34	30142.0
35	34409.0
36	38676.0
37	45183.5
38	51691.0
39	55754.0
40	59817.0
41	63708.5
42	67600.0
43	69483.5
44	71367.0
45	76541.5
46	81716.0
47	86789.0
48	91862.0
49	94187.0
50	96512.0
51	91349.0
52	86186.0
53	85246.5
54	84307.0
55	85791.5
56	87276.0
57	84721.5
58	82167.0
59	75356.0
60	68545.0
61	60053.5
62	51562.0
63	44173.5
64	36785.0
65	31536.5
66	26288.0
67	23188.0
68	20088.0
69	18427.5
70	16767.0
71	13099.5
72	9432.0
73	8323.0
74	7214.0
75	5204.5
76	3195.0
77	2840.5
78	2486.0
79	2264.5
80	2043.0
81	1592.5
82	1142.0
83	964.5
84	787.0
85	614.5
86	442.0
87	309.0
88	176.0
89	111.5
90	47.0
91	38.5
92	30.0
93	27.0
94	24.0
95	20.0
96	16.0
97	17.5
98	19.0
99	14.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02728478233027951
2	0.004534205809988139
3	3.1818988140267646E-4
4	0.0
5	7.954747035066911E-5
6	2.3864241105200733E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.159272331560219E-4
17	0.0
18	0.0
19	0.0
20	4.7728482210401466E-4
21	7.954747035066911E-5
22	0.0
23	0.0
24	8.750221738573603E-4
25	0.0
26	2.3864241105200733E-4
27	3.1818988140267646E-4
28	6.363797628053529E-4
29	2.3864241105200733E-4
30	6.363797628053529E-4
31	1.5909494070133823E-4
32	4.7728482210401466E-4
33	7.954747035066911E-5
34	7.954747035066912E-4
35	3.977373517533456E-4
36	3.1818988140267646E-4
37	0.0015114019366627132
38	0.0
39	4.7728482210401466E-4
40	0.0
41	9.545696442080293E-4
42	0.004375110869286801
43	0.0036591836361307794
44	0.00357963616578011
45	0.0018295918180653897
46	0.0016704968773640512
47	7.159272331560219E-4
48	0.0028637089326240877
49	0.00357963616578011
50	0.0014318544663120439
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1257111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.156835066696196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97403302740538	40.385391429895165
2	14.423545249587958	15.33420031818753
3	4.513148321241424	7.1971404283130145
4	1.8720935851108247	3.9805827973262433
5	0.9281638330919136	2.4669125895269692
6	0.5166764756291574	1.6478931718716598
7	0.32394933368187984	1.2054084910345673
8	0.23311089679266148	0.9913149994445716
9	0.17246567828129314	0.8250956653558128
>10	0.8742106578953317	8.499594689477643
>50	0.07764758155463407	2.870351579175876
>100	0.07787583832983482	8.630332310963245
>500	0.009922395543080007	3.5615198676571556
>1k	0.0031571258546163657	2.404261661770532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2161	0.17190208342779595	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1807	0.1437422789236591	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1755	0.13960581046542428	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1720	0.13682164900315086	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1637	0.13021920896404535	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1611	0.12815097473492792	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1590	0.1264804778575639	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1572	0.12504862339125186	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1517	0.12067351252196504	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1487	0.11828708841144497	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1469	0.11685523394513292	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1418	0.1127983129572488	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1299	0.10333216398551918	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1264	0.10054800252324576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.954747035066911E-5
2	0.0	0.0	0.0	0.0	7.954747035066911E-5
3	0.0	0.0	0.0	0.0	7.954747035066911E-5
4	0.0	0.0	0.0	0.0	7.954747035066911E-5
5	0.0	0.0	0.0	0.0	7.954747035066911E-5
6	0.0	0.0	0.0	0.0	1.5909494070133823E-4
7	0.0	0.0	0.0	0.0	1.5909494070133823E-4
8	0.0	0.0	0.0	0.0	1.5909494070133823E-4
9	0.0	0.0	0.0	1.5909494070133823E-4	1.5909494070133823E-4
10	0.0	0.0	0.0	1.5909494070133823E-4	1.5909494070133823E-4
11	0.0	0.0	0.0	1.5909494070133823E-4	1.5909494070133823E-4
12	0.0	0.0	0.0	1.5909494070133823E-4	2.3864241105200733E-4
13	0.0	0.0	0.0	1.5909494070133823E-4	2.3864241105200733E-4
14	0.0	0.0	0.0	1.5909494070133823E-4	2.3864241105200733E-4
15	0.0	0.0	0.0	1.5909494070133823E-4	3.1818988140267646E-4
16	0.0	0.0	0.0	1.5909494070133823E-4	3.1818988140267646E-4
17	0.0	0.0	0.0	1.5909494070133823E-4	3.1818988140267646E-4
18	0.0	0.0	0.0	2.3864241105200733E-4	3.977373517533456E-4
19	0.0	0.0	0.0	2.3864241105200733E-4	3.977373517533456E-4
20	0.0	0.0	0.0	3.1818988140267646E-4	4.7728482210401466E-4
21	0.0	0.0	0.0	3.1818988140267646E-4	5.568322924546838E-4
22	0.0	0.0	0.0	3.1818988140267646E-4	5.568322924546838E-4
23	0.0	0.0	0.0	3.977373517533456E-4	5.568322924546838E-4
24	0.0	0.0	0.0	3.977373517533456E-4	5.568322924546838E-4
25	0.0	0.0	0.0	3.977373517533456E-4	5.568322924546838E-4
26	0.0	0.0	0.0	4.7728482210401466E-4	7.15927233156022E-4
27	0.0	0.0	0.0	4.7728482210401466E-4	7.15927233156022E-4
28	0.0	0.0	0.0	7.15927233156022E-4	7.15927233156022E-4
29	0.0	0.0	0.0	0.0011136645849093676	7.15927233156022E-4
30	0.0	0.0	0.0	0.002068234229117397	7.15927233156022E-4
31	0.0	0.0	0.0	0.004295563398936132	7.15927233156022E-4
32	0.0	0.0	0.0	0.007557009683313565	7.15927233156022E-4
33	0.0	0.0	0.0	0.010898003438041669	7.15927233156022E-4
34	0.0	0.0	0.0	0.013841259841016426	7.15927233156022E-4
35	0.0	0.0	0.0	0.01686406371434185	7.15927233156022E-4
36	0.0	0.0	0.0	0.02243238663888869	7.15927233156022E-4
37	0.0	0.0	0.0	0.03126215584781296	7.15927233156022E-4
38	0.0	0.0	0.0	0.04295563398936132	7.15927233156022E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	580	0.0	28.456404	1
GTATCAA	1410	0.0	25.439892	1
GGCGTTA	480	0.0	20.167616	42
TGCGTAG	70	8.125346E-4	18.856531	13
TAGTACG	60	0.0074136523	18.332737	2
CGCAATA	305	0.0	18.033636	36
CCGTCGT	520	0.0	17.768652	9
CGTCGTA	520	0.0	17.768652	10
TAGGACG	260	0.0	17.768652	4
CGGCGTT	570	0.0	17.755222	41
GATATAC	350	0.0	17.605028	1
CGTTTAG	100	2.4838368E-5	17.599426	13
ACGGTAT	615	0.0	17.527885	9
TACGACG	590	0.0	17.524853	5
ACCGTCG	540	0.0	17.517948	8
ATACCGT	515	0.0	17.513992	6
CGACGGT	595	0.0	17.377586	7
TACCGTC	520	0.0	17.345589	7
GACGGTA	610	0.0	17.310913	8
CGCGGGA	350	0.0	16.970877	44
>>END_MODULE