FastQCFastQC Report
Thu 2 Feb 2017
SRR4062276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160303
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31691.9768812810739662No Hit
TATCAACGCAGAGTACTTTTTTTTT21611.3480720884824364No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14490.903913214350324No Hit
GTACATGGGGTGGTATCAACGCAAA12530.7816447602353043No Hit
GGTATCAACGCAGAGTACTTTTTTT12470.7779018483746405No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12360.7710398432967568No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10620.6624953993375046No Hit
GTATCAACGCAGAGTACATGGGGTG8230.5134027435543939No Hit
TATCAACGCAGAGTACATGGGGTGG5740.35807190133684336No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5070.31627605222609684No Hit
GGTATCAACGCAGAGTACATGGGGT4760.29693767427933354No Hit
ACGCAGAGTACATGGGGTGGTATCA4340.27073729125468643No Hit
GTACATGGGTGGTATCAACGCAAAA4300.2682420166809105No Hit
ATCAACGCAGAGTACTTTTTTTTTT3810.23767490315215561No Hit
GTACATGGGAGTGGTATCAACGCAA2890.18028358795530963No Hit
GTATCAACGCAGAGTACATGGGTGG2110.13162573376667935No Hit
GAGTACATGGGGTGGTATCAACGCA1970.12289227275846365No Hit
GTATCAACGCAGAGTACATGGGAGT1860.11603026768057992No Hit
CAACGCAGAGTACTTTTTTTTTTTT1830.11415881175024797No Hit
ATCAACGCAGAGTACATGGGGTGGT1760.10979208124614012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACC250.006012993418.9978164
TAAGACT250.006012993418.9978164
AATAAAG250.006012993418.9978162
GTCCTAC500.00149062213.29847051
GATATAC500.00149062213.29847051
TCCTACA550.003050462312.0895192
ATATACA550.003050462312.0895192
TTTCTCA855.26003E-511.17518416
GACAGTG600.005828178511.0855197
AACAGTG600.005828178511.0855197
CTACAGT1002.3647384E-510.4487984
TACAGTG1356.296141E-79.85071855
CATTTCT800.00448248639.49890714
ATTTCTC850.0073812418.94014715
GTATCAA14850.08.7633021
ACGCAAA3250.08.18367419
AACGCAG17200.07.67644266
GGGGTGG4150.07.5557077
AACGCAA3757.2759576E-127.092517418
GGGTGGT4450.06.83280858
Produced by FastQC (version 0.11.5)