##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062276_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160303
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.575154551068913	32.0	32.0	32.0	32.0	32.0
2	30.53924754995228	32.0	32.0	32.0	32.0	32.0
3	30.62802318110079	32.0	32.0	32.0	32.0	32.0
4	30.468456610294254	32.0	32.0	32.0	32.0	32.0
5	30.651534905772195	32.0	32.0	32.0	32.0	32.0
6	34.10995427409343	36.0	36.0	36.0	32.0	36.0
7	33.87308409699132	36.0	36.0	36.0	32.0	36.0
8	33.63591448694036	36.0	36.0	36.0	27.0	36.0
9	33.57249708364784	36.0	36.0	36.0	21.0	36.0
10	33.59417478150752	36.0	36.0	36.0	27.0	36.0
11	34.005944991672024	36.0	36.0	36.0	32.0	36.0
12	33.78603644348515	36.0	36.0	36.0	32.0	36.0
13	33.86798126048795	36.0	36.0	36.0	32.0	36.0
14	33.806928129854086	36.0	36.0	36.0	32.0	36.0
15	33.87831793540982	36.0	36.0	36.0	32.0	36.0
16	33.85712057790559	36.0	36.0	36.0	32.0	36.0
17	33.73006119660892	36.0	36.0	36.0	32.0	36.0
18	33.85274760921505	36.0	36.0	36.0	32.0	36.0
19	33.71389181737086	36.0	36.0	36.0	32.0	36.0
20	33.482230525941496	36.0	36.0	36.0	21.0	36.0
21	33.4292308939945	36.0	36.0	36.0	21.0	36.0
22	33.50305983044609	36.0	36.0	36.0	21.0	36.0
23	33.620556071938765	36.0	36.0	36.0	27.0	36.0
24	33.669756648347196	36.0	36.0	36.0	27.0	36.0
25	33.37820252896078	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	7.0
6	40.0
7	2.0
8	34.0
9	22.0
10	17.0
11	0.0
12	9.0
13	16.0
14	128.0
15	176.0
16	193.0
17	243.0
18	339.0
19	469.0
20	647.0
21	861.0
22	1104.0
23	1412.0
24	1716.0
25	2032.0
26	2451.0
27	2822.0
28	3392.0
29	4253.0
30	5425.0
31	7130.0
32	9860.0
33	13863.0
34	31006.0
35	70633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.41516896338763	17.100953830606173	12.393559615972451	22.09031759003375
2	15.475885989482286	20.80398749851841	39.8275743757057	23.8925521362936
3	19.125775099499695	27.887362603086675	28.060161445272048	24.926700852141582
4	11.741769656437356	15.811867166590762	34.283272298507086	38.16309087846479
5	12.975894066183674	39.59288865592102	30.948324815445773	16.482892462449534
6	31.331265098220364	35.814383181128704	17.926230173344734	14.928121547306198
7	28.654046728329906	33.32438154599449	19.39769043886658	18.62388128680902
8	29.72395703222583	31.389582812890332	18.494254309268047	20.392205845615788
9	29.36047419473714	13.819228871414028	19.261975103527103	37.55832183032173
10	17.586351227252567	28.387048409163317	29.182310570802073	24.844289792782043
11	34.292943491706495	23.341142660794052	22.610189610470748	19.75572423702871
12	26.589165688013793	25.876414882186854	28.2133130106694	19.321106419129958
13	30.345129150138987	21.681606646469064	26.38348377424493	21.589780429147016
14	24.503766254856156	20.082320462693463	28.643523665571557	26.77038961687882
15	23.875860517997477	27.99510238758605	26.42399330326466	21.705043791151812
16	21.48500690163453	27.95817796848358	27.270512844535215	23.286302285346675
17	20.495218344566528	27.056486623233035	27.29884877975651	25.149446252443923
18	20.93784350954332	26.256620365743977	31.134455880883383	21.67108024382932
19	23.297405987335924	24.485756035419453	30.935193395696214	21.281644581548413
20	24.95035779850886	23.20632422914091	30.447216914565463	21.396101057784772
21	26.30126379939058	22.828313102552574	29.596258554373346	21.2741645436835
22	24.77628530570699	23.705327313487825	29.665224151820002	21.85316322898518
23	23.094404175485256	24.5609434798624	30.79233079233079	21.55232155232155
24	22.370828260123005	25.41662764197184	30.84262121070213	21.36992288720302
25	22.3269085482733	25.977610029907407	30.774658000387113	20.920823421432182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	243.0
2	353.5
3	464.0
4	464.0
5	464.0
6	1126.5
7	1789.0
8	1789.0
9	1789.0
10	1673.5
11	1558.0
12	1558.0
13	1558.0
14	1183.5
15	809.0
16	809.0
17	809.0
18	1300.5
19	1792.0
20	1792.0
21	1792.0
22	2739.5
23	3687.0
24	3687.0
25	3687.0
26	5459.5
27	7232.0
28	7232.0
29	7232.0
30	8648.0
31	10064.0
32	10064.0
33	10064.0
34	10378.5
35	10693.0
36	10693.0
37	10693.0
38	11289.5
39	11886.0
40	11886.0
41	11886.0
42	13786.0
43	15686.0
44	15686.0
45	15686.0
46	18290.5
47	20895.0
48	20895.0
49	20895.0
50	21816.5
51	22738.0
52	22738.0
53	22738.0
54	19576.5
55	16415.0
56	16415.0
57	16415.0
58	14643.5
59	12872.0
60	12872.0
61	12872.0
62	11222.0
63	9572.0
64	9572.0
65	9572.0
66	7808.0
67	6044.0
68	6044.0
69	6044.0
70	4582.5
71	3121.0
72	3121.0
73	3121.0
74	2294.5
75	1468.0
76	1468.0
77	1468.0
78	1110.0
79	752.0
80	752.0
81	752.0
82	511.5
83	271.0
84	271.0
85	271.0
86	198.5
87	126.0
88	126.0
89	126.0
90	87.5
91	49.0
92	49.0
93	49.0
94	31.5
95	14.0
96	14.0
97	14.0
98	38.5
99	63.0
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012476372868879559
2	0.0012476372868879559
3	6.238186434439779E-4
4	0.007485823721327736
5	0.03243856945908685
6	0.06362950163128575
7	0.11603026768057992
8	0.11415881175024797
9	0.1247637286887956
10	0.14347828799211493
11	0.14846883713966677
12	0.13661628291423117
13	0.1359924642707872
14	0.12289227275846365
15	0.14035919477489503
16	0.12226845411501969
17	0.13224955241012332
18	0.11914936089779979
19	0.10293007616825638
20	0.09793952702070455
21	0.09419661516004067
22	0.10355389481170034
23	0.08109642364771713
24	0.09232515922970874
25	0.08858224736904487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	160303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.49412653495938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77050508282908	53.38211428174476
2	12.088927715580585	15.593296675212635
3	2.6975698043598277	5.21932224897805
4	0.8950063319724364	2.308906064952807
5	0.39499794801968535	1.2737523820315448
6	0.231998623725517	0.8977529156693953
7	0.168998989967675	0.7629609570278894
8	0.11199933033132542	0.5778639185775373
9	0.08199950969374965	0.4759638078593986
>10	0.47899713588912	5.812820867470328
>50	0.044999730929036835	1.9399717263230871
>100	0.023999856495486315	3.060872943194605
>500	0.0029999820619357894	1.227960826768337
>1k	0.006999958144516841	7.466440384189621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3169	1.9768812810739662	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2161	1.3480720884824364	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1449	0.903913214350324	No Hit
GTACATGGGGTGGTATCAACGCAAA	1253	0.7816447602353043	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1247	0.7779018483746405	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1236	0.7710398432967568	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1062	0.6624953993375046	No Hit
GTATCAACGCAGAGTACATGGGGTG	823	0.5134027435543939	No Hit
TATCAACGCAGAGTACATGGGGTGG	574	0.35807190133684336	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	507	0.31627605222609684	No Hit
GGTATCAACGCAGAGTACATGGGGT	476	0.29693767427933354	No Hit
ACGCAGAGTACATGGGGTGGTATCA	434	0.27073729125468643	No Hit
GTACATGGGTGGTATCAACGCAAAA	430	0.2682420166809105	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	381	0.23767490315215561	No Hit
GTACATGGGAGTGGTATCAACGCAA	289	0.18028358795530963	No Hit
GTATCAACGCAGAGTACATGGGTGG	211	0.13162573376667935	No Hit
GAGTACATGGGGTGGTATCAACGCA	197	0.12289227275846365	No Hit
GTATCAACGCAGAGTACATGGGAGT	186	0.11603026768057992	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	183	0.11415881175024797	No Hit
ATCAACGCAGAGTACATGGGGTGGT	176	0.10979208124614012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACC	25	0.0060129934	18.997816	4
TAAGACT	25	0.0060129934	18.997816	4
AATAAAG	25	0.0060129934	18.997816	2
GTCCTAC	50	0.001490622	13.2984705	1
GATATAC	50	0.001490622	13.2984705	1
TCCTACA	55	0.0030504623	12.089519	2
ATATACA	55	0.0030504623	12.089519	2
TTTCTCA	85	5.26003E-5	11.175184	16
GACAGTG	60	0.0058281785	11.085519	7
AACAGTG	60	0.0058281785	11.085519	7
CTACAGT	100	2.3647384E-5	10.448798	4
TACAGTG	135	6.296141E-7	9.8507185	5
CATTTCT	80	0.0044824863	9.498907	14
ATTTCTC	85	0.007381241	8.940147	15
GTATCAA	1485	0.0	8.763302	1
ACGCAAA	325	0.0	8.183674	19
AACGCAG	1720	0.0	7.6764426	6
GGGGTGG	415	0.0	7.555707	7
AACGCAA	375	7.2759576E-12	7.0925174	18
GGGTGGT	445	0.0	6.8328085	8
>>END_MODULE