FastQCFastQC Report
Thu 2 Feb 2017
SRR4062276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160303
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.6144613637923183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.41920612839435323No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5380.33561443017286013No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5360.3343667928859722No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4920.3069187725744371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.29693767427933354No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4130.25763709974236293No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA3700.23081289807427183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3230.20149342183240487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2610.16281666593887825No Hit
ATATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2220.13848773884456309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1870.11665408632402388No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1850.11540644903713591No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1750.10916826260269615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1690.10542535074203228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1670.10417771345514432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG250.002346729435.241
GAACGAA250.002346729435.226
ACTATAC353.2055215E-431.4285723
CTAGAGT300.005730349629.3333364
ACGTTTT300.005730349629.33333629
CACGAAT300.005730349629.33333614
TTAAGAC300.005730349629.3333363
ATACATG1800.026.888891
GACGTGG609.684292E-625.6666687
AGAAAAC1151.2732926E-1124.86956623
AGGGTGA450.001392936224.4444456
GCCCGAG450.001392936224.44444512
TAGGACC551.5852263E-424.04
GTAGGAC852.3263237E-723.2941193
CTGTAGG904.0363193E-722.01
AGGACCG500.00257077622.05
AAAATAG500.00257077622.034
GGAATAT956.79016E-720.84210612
TGTAGGA956.79016E-720.8421062
ATGGGAC654.9065443E-420.30769312
Produced by FastQC (version 0.11.5)