##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160303
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.707354197987563	32.0	32.0	32.0	32.0	32.0
2	31.185486235441633	32.0	32.0	32.0	32.0	32.0
3	31.426292708183876	32.0	32.0	32.0	32.0	32.0
4	31.647292938996774	32.0	32.0	32.0	32.0	32.0
5	31.608834519628452	32.0	32.0	32.0	32.0	32.0
6	35.18562347554319	36.0	36.0	36.0	36.0	36.0
7	35.16151288497408	36.0	36.0	36.0	36.0	36.0
8	35.13087715139454	36.0	36.0	36.0	36.0	36.0
9	35.10396561511637	36.0	36.0	36.0	36.0	36.0
10	35.05700454763791	36.0	36.0	36.0	36.0	36.0
11	35.17969096024404	36.0	36.0	36.0	36.0	36.0
12	35.088644629233386	36.0	36.0	36.0	36.0	36.0
13	35.11691609015427	36.0	36.0	36.0	36.0	36.0
14	35.044072787159315	36.0	36.0	36.0	36.0	36.0
15	35.09051608516372	36.0	36.0	36.0	36.0	36.0
16	35.092955216059586	36.0	36.0	36.0	36.0	36.0
17	35.06365445437702	36.0	36.0	36.0	36.0	36.0
18	35.05862023792443	36.0	36.0	36.0	36.0	36.0
19	34.997068052375816	36.0	36.0	36.0	36.0	36.0
20	34.87763797308846	36.0	36.0	36.0	32.0	36.0
21	34.838399780415834	36.0	36.0	36.0	32.0	36.0
22	34.890813022838	36.0	36.0	36.0	32.0	36.0
23	34.92666388027673	36.0	36.0	36.0	36.0	36.0
24	34.93386274742207	36.0	36.0	36.0	36.0	36.0
25	34.947780141357306	36.0	36.0	36.0	36.0	36.0
26	34.93429318228605	36.0	36.0	36.0	36.0	36.0
27	34.935328721234164	36.0	36.0	36.0	36.0	36.0
28	34.91460546589895	36.0	36.0	36.0	36.0	36.0
29	34.86369562640749	36.0	36.0	36.0	32.0	36.0
30	34.85818730778588	36.0	36.0	36.0	32.0	36.0
31	34.80798238336151	36.0	36.0	36.0	32.0	36.0
32	34.72775306762818	36.0	36.0	36.0	32.0	36.0
33	34.62587100678091	36.0	36.0	36.0	32.0	36.0
34	34.52619726393143	36.0	36.0	36.0	32.0	36.0
35	34.48972258784926	36.0	36.0	36.0	32.0	36.0
36	34.4760172922528	36.0	36.0	36.0	32.0	36.0
37	34.44496983836859	36.0	36.0	36.0	32.0	36.0
38	34.39529515979115	36.0	36.0	36.0	32.0	36.0
39	34.28574012962952	36.0	36.0	36.0	32.0	36.0
40	34.25731895223421	36.0	36.0	36.0	32.0	36.0
41	34.15725220363936	36.0	36.0	36.0	32.0	36.0
42	34.06185161849747	36.0	36.0	36.0	32.0	36.0
43	33.96155405700455	36.0	36.0	36.0	32.0	36.0
44	33.83698371209522	36.0	36.0	36.0	32.0	36.0
45	33.66721146828194	36.0	36.0	36.0	27.0	36.0
46	33.46180670355514	36.0	36.0	36.0	21.0	36.0
47	33.24569721090685	36.0	36.0	36.0	14.0	36.0
48	33.0967979389032	36.0	36.0	36.0	14.0	36.0
49	32.96870301865842	36.0	36.0	36.0	14.0	36.0
50	32.53375170770354	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	9.0
22	28.0
23	56.0
24	162.0
25	369.0
26	765.0
27	1547.0
28	2555.0
29	3916.0
30	5249.0
31	6835.0
32	9397.0
33	13760.0
34	23800.0
35	91849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57837986247176	18.92510826292603	13.018382857642052	22.478129016960153
2	15.20499575784798	21.192668563158158	39.15069621200779	24.451639466986073
3	18.79514915596811	27.656548265149528	28.660278723908622	24.88802385497374
4	11.349132580176292	15.739568192735007	34.70240731614505	38.20889191094365
5	12.581798219621593	39.199515916732686	31.036848967268238	17.181836896377487
6	31.70059200389263	36.22015807564425	17.005920038926284	15.07332988153684
7	28.089929695638883	33.291953363318214	19.563576477046592	19.054540463996304
8	29.967623812405257	30.845336643730935	18.515561156060713	20.671478387803095
9	29.181612321665845	13.171930656319594	19.319663387459997	38.32679363455456
10	17.351515567394244	28.26834182766386	29.622652102580737	24.757490502361154
11	34.85461906514538	22.836129080553704	22.30401177769599	20.00524007660493
12	26.40998608884425	25.929021914748944	28.4972832698078	19.163708726599
13	30.499741115262967	21.377017273538236	26.65452299707429	21.4687186141245
14	24.553501805954973	19.56919084483759	29.10113971666157	26.776167632545867
15	23.8205148999083	27.49543052843677	27.20348340330499	21.48057116834994
16	22.213696647577695	26.916070916145774	27.489987648313807	23.38024478796272
17	20.732612614860606	25.71505212004766	28.57900351209896	24.97333175299277
18	21.12873745344753	24.271535779118295	33.056149916096395	21.54357685133778
19	23.54166796628884	22.85297218392669	32.903938167096065	20.70142168268841
20	24.54663980087709	22.101270718576696	32.2576620524881	21.094427428058115
21	25.29522217300986	21.701402968129106	31.96633874599976	21.03703611286127
22	23.99580793871606	22.598454177401546	31.930781083323456	21.474956800558942
23	22.505645593941438	23.18685980212349	32.93970131377026	21.367793290164812
24	22.352185250339986	23.38024478796272	33.0663372883682	21.20123267332909
25	22.392594025065033	23.79992888467465	32.99314423310855	20.81433285715177
26	21.31962595834139	24.783067066742355	33.30567737347398	20.591629601442268
27	21.159928385619732	24.7737097870907	33.14972271261299	20.91663911467658
28	21.09094765472455	24.7852477526653	33.01351831866302	21.11028627394714
29	20.752574811450817	24.721932839684847	32.799136634997474	21.726355713866866
30	20.77978789769183	25.41983780411728	32.66812227074236	21.132252027448533
31	21.05575067216459	25.17607281211206	32.140384147520635	21.627792368202716
32	20.796242116491896	25.570326194768654	31.990667673094077	21.642764015645373
33	21.14558055682052	24.99391776822642	32.173446535623164	21.687055139329896
34	21.816945515339796	25.196816009781536	32.09317413382241	20.893064341056256
35	21.492557797157865	25.566118950480966	32.05699242679442	20.884330825566742
36	21.013954823053844	26.722519229209684	31.303219528018815	20.960306419717657
37	21.19624209304937	26.713952414817033	31.487442452371152	20.602363039762448
38	21.116261080578656	27.223445599895197	31.28388115007205	20.3764121694541
39	21.367036175243133	26.90841718495599	31.303219528018815	20.421327111782063
40	21.322121232915165	27.4149579234325	30.998796030018156	20.26412481363418
41	20.805105363626154	27.59229454404811	31.239784905989943	20.36281518633579
42	21.25357938275533	28.047388220321533	30.433019532980232	20.266012863942905
43	20.945364259067485	28.249260751849683	30.02719934122696	20.778175647855868
44	20.654160714174314	28.530789721579318	29.379838674460533	21.435210889785836
45	21.157205240174672	28.636306924516532	28.840923268870867	21.36556456643793
46	21.313919612723723	28.696997485948135	28.01639436303408	21.972688538294065
47	22.101822219726884	29.125571588094743	27.448081397887698	21.32452479429067
48	22.105079289822704	29.848157806086164	26.470074486269326	21.576688417821806
49	21.927619142684243	30.176990598349253	26.20733540872538	21.688054850241123
50	21.600479095706852	30.850519644171627	25.977841823558034	21.571159436563487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	67.0
2	57.0
3	160.0
4	263.0
5	262.5
6	262.0
7	225.0
8	188.0
9	282.0
10	376.0
11	589.0
12	802.0
13	1569.5
14	2337.0
15	3040.0
16	3743.0
17	3881.5
18	4020.0
19	3871.5
20	3723.0
21	3609.0
22	3495.0
23	3110.0
24	2725.0
25	2292.0
26	1859.0
27	1971.5
28	2084.0
29	2442.5
30	2801.0
31	3511.5
32	4222.0
33	4785.5
34	5349.0
35	5487.5
36	5626.0
37	5765.5
38	5905.0
39	6041.0
40	6177.0
41	6618.5
42	7060.0
43	7280.0
44	7500.0
45	8048.0
46	8596.0
47	8788.0
48	8980.0
49	9296.5
50	9613.0
51	9599.5
52	9586.0
53	9591.0
54	9596.0
55	9479.0
56	9362.0
57	9096.5
58	8831.0
59	8061.5
60	7292.0
61	6500.0
62	5708.0
63	4806.5
64	3905.0
65	3375.0
66	2845.0
67	2402.0
68	1959.0
69	1619.0
70	1279.0
71	1021.5
72	764.0
73	669.5
74	575.0
75	423.5
76	272.0
77	231.5
78	191.0
79	165.5
80	140.0
81	107.0
82	74.0
83	60.0
84	46.0
85	35.0
86	24.0
87	14.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02807183895497901
2	0.004366730504107846
3	6.238186434439779E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.238186434439779E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	6.238186434439779E-4
24	6.238186434439779E-4
25	0.0
26	0.0
27	0.0
28	0.0012476372868879559
29	0.0
30	0.001871455930331934
31	0.0
32	0.0
33	0.0
34	6.238186434439779E-4
35	6.238186434439779E-4
36	0.0
37	6.238186434439779E-4
38	0.0
39	0.0
40	0.0
41	6.238186434439779E-4
42	0.00623818643443978
43	0.00311909321721989
44	0.003742911860663868
45	0.001871455930331934
46	0.0024952745737759118
47	0.0024952745737759118
48	0.00311909321721989
49	0.007485823721327736
50	6.238186434439779E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	160303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.76586193100881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83267629176156	55.45461843032451
2	12.76884210399572	17.30583182076452
3	2.8199114889420924	5.732811978519458
4	0.9381868684225719	2.5430816716403815
5	0.45600632891996534	1.5450830962628277
6	0.23747493653500001	0.9655616256783535
7	0.16663088439936874	0.7904319853954659
8	0.12472364904365599	0.6761604464499191
9	0.07982312984427681	0.4868354876335425
>10	0.4959011891170766	6.2876154904437636
>50	0.03592040805842193	1.6072332529675586
>100	0.0399115645093577	4.7581406820078715
>500	0.00399115645093577	1.8465940319118228
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.6144613637923183	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.41920612839435323	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	538	0.33561443017286013	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	536	0.3343667928859722	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	492	0.3069187725744371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.29693767427933354	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	413	0.25763709974236293	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	370	0.23081289807427183	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	323	0.20149342183240487	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	261	0.16281666593887825	No Hit
ATATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	222	0.13848773884456309	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	187	0.11665408632402388	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	185	0.11540644903713591	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	175	0.10916826260269615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	169	0.10542535074203228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	167	0.10417771345514432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	6.238186434439779E-4
16	0.0	0.0	0.0	0.0	6.238186434439779E-4
17	0.0	0.0	0.0	0.0	6.238186434439779E-4
18	0.0	0.0	0.0	0.0	6.238186434439779E-4
19	0.0	0.0	0.0	0.0	6.238186434439779E-4
20	0.0	0.0	0.0	0.0	6.238186434439779E-4
21	0.0	0.0	0.0	0.0	6.238186434439779E-4
22	0.0	0.0	0.0	6.238186434439779E-4	6.238186434439779E-4
23	0.0	0.0	0.0	0.0024952745737759118	6.238186434439779E-4
24	0.0	0.0	0.0	0.008109642364771713	6.238186434439779E-4
25	0.0	0.0	0.0	0.010604916938547626	6.238186434439779E-4
26	0.0	0.0	0.0	0.01247637286887956	6.238186434439779E-4
27	0.0	0.0	0.0	0.01497164744265547	6.238186434439779E-4
28	0.0	0.0	0.0	0.019338377946763317	6.238186434439779E-4
29	0.0	0.0	0.0	0.02994329488531094	6.238186434439779E-4
30	0.0	0.0	0.0	0.04990549147551824	6.238186434439779E-4
31	0.0	0.0	0.0	0.09731570837726056	6.238186434439779E-4
32	0.0	0.0	0.0	0.1334971896970113	6.238186434439779E-4
33	0.0	0.0	0.0	0.1584499354347704	6.238186434439779E-4
34	0.0	0.0	0.0	0.1921361421807452	6.238186434439779E-4
35	0.0	0.0	0.0	0.22831762350049595	6.238186434439779E-4
36	0.0	0.0	0.0	0.2863327573407859	6.238186434439779E-4
37	0.0	0.0	0.0	0.3424764352507439	6.238186434439779E-4
38	0.0	0.0	0.0	0.4104726673861375	6.238186434439779E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	25	0.0023467294	35.2	41
GAACGAA	25	0.0023467294	35.2	26
ACTATAC	35	3.2055215E-4	31.428572	3
CTAGAGT	30	0.0057303496	29.333336	4
ACGTTTT	30	0.0057303496	29.333336	29
CACGAAT	30	0.0057303496	29.333336	14
TTAAGAC	30	0.0057303496	29.333336	3
ATACATG	180	0.0	26.88889	1
GACGTGG	60	9.684292E-6	25.666668	7
AGAAAAC	115	1.2732926E-11	24.869566	23
AGGGTGA	45	0.0013929362	24.444445	6
GCCCGAG	45	0.0013929362	24.444445	12
TAGGACC	55	1.5852263E-4	24.0	4
GTAGGAC	85	2.3263237E-7	23.294119	3
CTGTAGG	90	4.0363193E-7	22.0	1
AGGACCG	50	0.002570776	22.0	5
AAAATAG	50	0.002570776	22.0	34
GGAATAT	95	6.79016E-7	20.842106	12
TGTAGGA	95	6.79016E-7	20.842106	2
ATGGGAC	65	4.9065443E-4	20.307693	12
>>END_MODULE