FastQCFastQC Report
Thu 2 Feb 2017
SRR4062275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222829
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8390.3765219069331191No Hit
TATCAACGCAGAGTACTTTTTTTTT5610.25176256232357547No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT3940.17681720063366976No Hit
GGTATCAACGCAGAGTACTTTTTTT3530.15841744117686657No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG3260.1463005264126303No Hit
CTCCACTCCTGGTGGTGCCCTTCCG2960.13283728778570114No Hit
ACCATACTCCCCCCGGAACCCAAAG2790.12520811923044128No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2730.12251547150505543No Hit
TCGTAGTTCCGACCATAAACGATGC2720.12206669688415782No Hit
GTCAATCCTGTCCGTGTCCGGGCCG2690.12072037302146489No Hit
GAATAGGACCGCGGTTCTATTTTGT2380.10680835977363809No Hit
GTATCTGATCGTCTTCGAACCTCCG2280.1023206135646617No Hit
CTATTGGAGCTGGAATTACCGCGGC2270.10187183894376406No Hit
GAACTACGACGGTATCTGATCGTCT2260.10142306432286642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTT307.687588E-419.00247413
TAGAGTG402.7450628E-416.6271655
TAGAACC402.7450628E-416.6271654
TTTCGTT350.002163714516.28417217
TTTAGAA350.002173487316.2731952
GGTATCA1550.015.3108031
GGGGACG508.650935E-515.201985
GCGGGGA704.3912405E-714.9271573
GGCGGGG704.4249464E-714.9170962
CGCATCG653.3363249E-614.61728713
CGGTGAC653.3363249E-614.61728714
GGTGACT653.3363249E-614.61728715
TAGAGCA400.005255759714.2518555
GGCGGTG602.5456655E-514.25185512
CGCCAGT602.5510737E-514.24865118
CCTTATA400.00528722214.2390482
CGGGGAC759.551859E-713.9351474
GTGACTC551.9461401E-413.81998216
CCGCATC707.186176E-613.57319512
AGAGCGA707.186176E-613.57319515
Produced by FastQC (version 0.11.5)