##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062275_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222829
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.573740401832797	32.0	21.0	32.0	14.0	32.0
2	25.39716553949441	32.0	14.0	32.0	14.0	32.0
3	25.74259634069174	32.0	14.0	32.0	14.0	32.0
4	25.397156564001993	32.0	14.0	32.0	14.0	32.0
5	25.790206840222773	32.0	21.0	32.0	14.0	32.0
6	28.97096428202792	32.0	21.0	36.0	14.0	36.0
7	28.782977081080112	32.0	21.0	36.0	14.0	36.0
8	28.51768396393647	32.0	21.0	36.0	14.0	36.0
9	28.6997293889036	32.0	21.0	36.0	14.0	36.0
10	27.48244169295738	32.0	21.0	36.0	14.0	36.0
11	29.48256734985123	36.0	21.0	36.0	14.0	36.0
12	28.22460721001306	32.0	21.0	36.0	14.0	36.0
13	28.759726067971403	32.0	21.0	36.0	14.0	36.0
14	28.06370355743642	32.0	21.0	36.0	14.0	36.0
15	28.125966548339758	32.0	21.0	36.0	14.0	36.0
16	28.21841412024467	32.0	21.0	36.0	14.0	36.0
17	27.918951303465885	32.0	21.0	36.0	14.0	36.0
18	28.00815872260792	32.0	21.0	36.0	14.0	36.0
19	28.01420820449762	32.0	21.0	36.0	14.0	36.0
20	27.979760264597516	32.0	21.0	36.0	14.0	36.0
21	27.98592194014244	32.0	21.0	36.0	14.0	36.0
22	27.741739181165826	32.0	21.0	36.0	14.0	36.0
23	27.624698760035724	32.0	14.0	36.0	14.0	36.0
24	27.531394028604893	32.0	14.0	36.0	14.0	36.0
25	26.709288288328718	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	29.0
6	35.0
7	19.0
8	28.0
9	13.0
10	5.0
11	4.0
12	5.0
13	8.0
14	314.0
15	704.0
16	1435.0
17	3045.0
18	5144.0
19	7724.0
20	9933.0
21	11459.0
22	11953.0
23	11746.0
24	11464.0
25	11485.0
26	11451.0
27	11576.0
28	11991.0
29	12403.0
30	12266.0
31	13493.0
32	14540.0
33	15247.0
34	18204.0
35	15090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75116793595088	18.695053157353843	11.759241391380835	26.79453751531443
2	18.13543120509449	18.730506352404756	37.85324172347405	25.280820719026696
3	18.55511874629759	22.691492990108962	29.169583714793472	29.583804548799968
4	12.952058667767735	15.366179863024765	35.586632796861956	36.095128672345545
5	16.097913524384115	35.203889248006895	32.78298498886734	15.915212238741649
6	33.82797311434485	35.90456221011939	16.012410145428586	14.255054530107175
7	29.93496402360698	30.37467549383326	20.41267303252697	19.277687450032786
8	28.08370538722051	32.086615941573314	19.171929320241826	20.657749350964348
9	26.36292992314499	13.801560457626433	19.127060060100526	40.70844955912805
10	16.32953754239382	25.519843676302134	30.58554005794758	27.565078723356468
11	36.844658885684254	21.312793696146347	21.93365499519304	19.90889242297636
12	24.052469621077694	23.590215853192873	28.614361761865187	23.742952763864245
13	28.373777031094182	19.421509878084937	25.63810002964773	26.56661306117315
14	23.12543231613651	19.712717505547122	25.25489808568169	31.906952092634683
15	25.420477619449784	26.03502183249178	22.145154624355357	26.399345923703077
16	25.514636194993557	26.034774718932052	23.674387893977983	24.77620119209641
17	22.87424934535279	26.453137141291506	25.465888725694956	25.206724787660743
18	23.71575765469972	25.03851491863583	26.722840806500148	24.5228866201643
19	25.1841405576315	24.589052170163843	25.43969172175913	24.78711555044553
20	25.25790095345881	23.79874518891778	25.239487476590185	25.70386638103322
21	25.928953159383845	23.583778685947816	25.33390218709301	25.153365967575336
22	24.617678629975792	24.22783434312585	25.679420443470335	25.475066583428024
23	23.644691934614695	24.461110113166875	26.383599784444044	25.510598167774383
24	23.90691240928361	25.09835093770209	25.824082057914783	25.170654595099517
25	24.13830269770199	24.092944298988193	25.826892590413657	25.941860412896155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	19.0
2	53.0
3	87.0
4	87.0
5	87.0
6	224.5
7	362.0
8	362.0
9	362.0
10	408.0
11	454.0
12	454.0
13	454.0
14	595.5
15	737.0
16	737.0
17	737.0
18	1248.5
19	1760.0
20	1760.0
21	1760.0
22	2793.5
23	3827.0
24	3827.0
25	3827.0
26	5381.0
27	6935.0
28	6935.0
29	6935.0
30	8506.0
31	10077.0
32	10077.0
33	10077.0
34	11900.5
35	13724.0
36	13724.0
37	13724.0
38	15560.0
39	17396.0
40	17396.0
41	17396.0
42	19783.0
43	22170.0
44	22170.0
45	22170.0
46	24654.0
47	27138.0
48	27138.0
49	27138.0
50	28420.5
51	29703.0
52	29703.0
53	29703.0
54	27837.5
55	25972.0
56	25972.0
57	25972.0
58	23736.5
59	21501.0
60	21501.0
61	21501.0
62	19002.0
63	16503.0
64	16503.0
65	16503.0
66	13943.5
67	11384.0
68	11384.0
69	11384.0
70	8872.0
71	6360.0
72	6360.0
73	6360.0
74	4761.0
75	3162.0
76	3162.0
77	3162.0
78	2631.0
79	2100.0
80	2100.0
81	2100.0
82	1449.0
83	798.0
84	798.0
85	798.0
86	583.5
87	369.0
88	369.0
89	369.0
90	232.5
91	96.0
92	96.0
93	96.0
94	60.0
95	24.0
96	24.0
97	24.0
98	97.5
99	171.0
100	171.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.48774620897639E-4
4	0.006731619313464585
5	0.027375251874755976
6	0.048467659056945014
7	0.08212575562426794
8	0.08571595259144904
9	0.09065247342132308
10	0.09603776887209474
11	0.10546203591094516
12	0.10052551508107115
13	0.09648654349299239
14	0.08571595259144904
15	0.10097428970196878
16	0.08795982569593724
17	0.0852671779705514
18	0.08436962872875613
19	0.07763800941529155
20	0.07449658706900807
21	0.0713551647227246
22	0.07898433327798446
23	0.06686741851374821
24	0.07045761548092933
25	0.07090639010182696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	222829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.80239947759368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70114083354774	45.03317299091178
2	11.572920647307107	12.915934821670463
3	2.837479357467364	4.75014469844459
4	1.2865389963110814	2.8716785206261353
5	0.717770574600075	2.002666016854767
6	0.48579439356535215	1.6265095688225484
7	0.3364017288328068	1.3140416560096997
8	0.2593777046896568	1.1579118634138843
9	0.2120182876395568	1.0648016265076121
>10	1.374605009732481	14.72604374229898
>50	0.14495439519222286	5.517444105325909
>100	0.06902590247248708	6.249288192299426
>500	0.0019721686420710594	0.7703621968142288
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	839	0.3765219069331191	No Hit
TATCAACGCAGAGTACTTTTTTTTT	561	0.25176256232357547	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	394	0.17681720063366976	No Hit
GGTATCAACGCAGAGTACTTTTTTT	353	0.15841744117686657	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	326	0.1463005264126303	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	296	0.13283728778570114	No Hit
ACCATACTCCCCCCGGAACCCAAAG	279	0.12520811923044128	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	273	0.12251547150505543	No Hit
TCGTAGTTCCGACCATAAACGATGC	272	0.12206669688415782	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	269	0.12072037302146489	No Hit
GAATAGGACCGCGGTTCTATTTTGT	238	0.10680835977363809	No Hit
GTATCTGATCGTCTTCGAACCTCCG	228	0.1023206135646617	No Hit
CTATTGGAGCTGGAATTACCGCGGC	227	0.10187183894376406	No Hit
GAACTACGACGGTATCTGATCGTCT	226	0.10142306432286642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.48774620897639E-4	0.0	0.0	0.0	0.0
7	4.48774620897639E-4	0.0	0.0	4.48774620897639E-4	0.0
8	4.48774620897639E-4	0.0	0.0	4.48774620897639E-4	0.0
9	4.48774620897639E-4	0.0	0.0	8.97549241795278E-4	0.0
10	4.48774620897639E-4	0.0	0.0	8.97549241795278E-4	0.0
11	4.48774620897639E-4	0.0	0.0	8.97549241795278E-4	0.0
12	4.48774620897639E-4	0.0	0.0	8.97549241795278E-4	0.0
13	4.48774620897639E-4	0.0	0.0	8.97549241795278E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTTT	30	7.687588E-4	19.002474	13
TAGAGTG	40	2.7450628E-4	16.627165	5
TAGAACC	40	2.7450628E-4	16.627165	4
TTTCGTT	35	0.0021637145	16.284172	17
TTTAGAA	35	0.0021734873	16.273195	2
GGTATCA	155	0.0	15.310803	1
GGGGACG	50	8.650935E-5	15.20198	5
GCGGGGA	70	4.3912405E-7	14.927157	3
GGCGGGG	70	4.4249464E-7	14.917096	2
CGCATCG	65	3.3363249E-6	14.617287	13
CGGTGAC	65	3.3363249E-6	14.617287	14
GGTGACT	65	3.3363249E-6	14.617287	15
TAGAGCA	40	0.0052557597	14.251855	5
GGCGGTG	60	2.5456655E-5	14.251855	12
CGCCAGT	60	2.5510737E-5	14.248651	18
CCTTATA	40	0.005287222	14.239048	2
CGGGGAC	75	9.551859E-7	13.935147	4
GTGACTC	55	1.9461401E-4	13.819982	16
CCGCATC	70	7.186176E-6	13.573195	12
AGAGCGA	70	7.186176E-6	13.573195	15
>>END_MODULE