FastQCFastQC Report
Thu 2 Feb 2017
SRR4062275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222829
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG3950.1772659752545674No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG3770.1691880320784099No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC3760.16873925745751225No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3020.135529935511087No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2670.11982282377966962No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC2630.11802772529607905No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT2570.11533507757069324No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG2560.11488630294979559No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2540.11398875370800031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGC250.00234951235.1974873
GGTAGTG651.7345883E-830.45936416
CTCACGA300.005737092329.3312418
CGAACTG300.005737092329.3312410
GTATCAA1900.028.9453011
ATACGAA703.5577614E-828.29004540
TAGTGAC703.565583E-828.28369518
TACGAAT804.269168E-927.50420841
AGTGACG655.898437E-727.07499119
GTGACGA655.898437E-727.07499120
AGGTAGT756.963637E-826.39811515
TAGGGAC508.315524E-526.3981153
GTAGTGA756.963637E-826.39811517
CGAAAAA701.126049E-625.14106424
ACGAAAA701.126049E-625.14106423
TCCCGAC801.3007411E-724.7482322
GGAGGTA801.3007411E-724.74823213
GAGTAAG450.001395221924.4426981
GCAGTTA450.001395221924.44269815
TTCGAGT450.001395221924.44269826
Produced by FastQC (version 0.11.5)