##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222829
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.163739908180712	32.0	32.0	32.0	21.0	32.0
2	28.743843934137836	32.0	32.0	32.0	14.0	32.0
3	30.346902781953876	32.0	32.0	32.0	21.0	32.0
4	30.913242890288068	32.0	32.0	32.0	32.0	32.0
5	30.618380013373482	32.0	32.0	32.0	32.0	32.0
6	34.179657943983955	36.0	36.0	36.0	32.0	36.0
7	34.182673709436386	36.0	36.0	36.0	32.0	36.0
8	34.04271436841704	36.0	36.0	36.0	32.0	36.0
9	34.38796565976601	36.0	36.0	36.0	32.0	36.0
10	33.91094516422907	36.0	36.0	36.0	32.0	36.0
11	34.60744337586221	36.0	36.0	36.0	32.0	36.0
12	34.20667417616199	36.0	36.0	36.0	32.0	36.0
13	34.41145901117	36.0	36.0	36.0	32.0	36.0
14	34.25082013561969	36.0	36.0	36.0	32.0	36.0
15	34.17156204982296	36.0	36.0	36.0	32.0	36.0
16	34.2019799936274	36.0	36.0	36.0	32.0	36.0
17	34.152201912677434	36.0	36.0	36.0	32.0	36.0
18	34.20476688402317	36.0	36.0	36.0	32.0	36.0
19	34.198313504974664	36.0	36.0	36.0	32.0	36.0
20	34.215856104905555	36.0	36.0	36.0	32.0	36.0
21	34.195118229673874	36.0	36.0	36.0	32.0	36.0
22	34.1515332384923	36.0	36.0	36.0	32.0	36.0
23	34.072315542411445	36.0	36.0	36.0	32.0	36.0
24	34.06268483904698	36.0	36.0	36.0	32.0	36.0
25	34.00498139829197	36.0	36.0	36.0	32.0	36.0
26	33.93664648676788	36.0	36.0	36.0	32.0	36.0
27	33.93513411629546	36.0	36.0	36.0	32.0	36.0
28	33.825408721485985	36.0	36.0	36.0	32.0	36.0
29	33.78591206710078	36.0	36.0	36.0	32.0	36.0
30	33.766677586849106	36.0	36.0	36.0	32.0	36.0
31	33.76114868351965	36.0	36.0	36.0	32.0	36.0
32	33.710567295998274	36.0	36.0	36.0	32.0	36.0
33	33.66665469934344	36.0	36.0	36.0	27.0	36.0
34	33.616625304605776	36.0	36.0	36.0	27.0	36.0
35	33.62047130310687	36.0	36.0	36.0	27.0	36.0
36	33.51652163766835	36.0	36.0	36.0	21.0	36.0
37	33.566645275076404	36.0	36.0	36.0	27.0	36.0
38	33.488872633274845	36.0	36.0	36.0	21.0	36.0
39	33.47854184150178	36.0	36.0	36.0	21.0	36.0
40	33.45258022968285	36.0	36.0	36.0	21.0	36.0
41	33.39085576832459	36.0	36.0	36.0	21.0	36.0
42	33.32214837386516	36.0	36.0	36.0	21.0	36.0
43	33.354729411342326	36.0	36.0	36.0	21.0	36.0
44	33.22695878902656	36.0	36.0	36.0	21.0	36.0
45	33.192255047592546	36.0	36.0	36.0	21.0	36.0
46	33.11916761283316	36.0	36.0	36.0	21.0	36.0
47	33.17437586669599	36.0	36.0	36.0	21.0	36.0
48	33.055621126514055	36.0	36.0	36.0	14.0	36.0
49	33.05295540526592	36.0	36.0	36.0	14.0	36.0
50	32.38566793370701	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	16.0
22	34.0
23	76.0
24	258.0
25	559.0
26	1133.0
27	2155.0
28	4049.0
29	7068.0
30	11193.0
31	18025.0
32	27354.0
33	42079.0
34	60263.0
35	48560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02810731229998	18.6907791677327	13.16986027137785	26.11125324858947
2	19.034048836092573	20.544188989664626	34.880871707647636	25.54089046659516
3	21.53400140915231	22.93283190249025	28.255747680957143	27.277419007400294
4	16.047731668678672	16.589402636102125	35.70630393709975	31.656561758119455
5	20.434054813332196	32.22471042817587	30.502762207791623	16.838472550700313
6	35.102253297371526	36.51185438161101	14.373353558109581	14.01253876290788
7	30.37216879311041	30.135664567897358	19.06259957186901	20.42956706712322
8	28.74087304614749	32.20810576720265	17.45688397829726	21.594137208352592
9	26.472766112130824	13.24109518958484	17.144088067531605	43.142050630752735
10	16.594339156931998	25.141251811927535	29.3292165741443	28.935192456996173
11	39.102181493432184	20.079074088202166	20.707807332079756	20.110937086285897
12	24.37968128026424	22.99162137782784	27.46007027810563	25.16862706380229
13	29.672080384510096	18.609337204762397	23.996427754017656	27.72215465670985
14	24.150357448985545	18.79109092622594	23.60374996073222	33.45480166405629
15	26.51405337725341	25.488603368502304	20.723065669190277	27.27427758505401
16	26.540650187588632	25.947367476259714	22.152961028237026	25.359021307914624
17	24.21049324818583	26.086371163537958	23.532843570630394	26.170292017645817
18	25.7750372484607	24.87568887213456	24.662519970560254	24.686753908844487
19	26.44987860646505	24.692028416408995	24.102787339170394	24.755305637955562
20	26.632978651791284	23.582208778929132	23.505917093376535	26.278895475903045
21	27.411602619048686	23.498287924821277	23.604647509974015	25.48546194615602
22	25.895193175035562	24.53764994682021	23.9493064188234	25.617850459320824
23	25.01065839724632	24.053422130871656	24.787617410660193	26.148302061221834
24	25.285421939792123	24.944351697273234	24.272981851472885	25.49724451146175
25	25.17580745773665	23.945267447235324	24.613941632372807	26.264983462655223
26	25.3317596162045	24.283412692357746	24.2609737599124	26.123853931525353
27	25.727351466819847	23.948857644202505	24.65478012287449	25.669010766103156
28	24.95781499632003	24.458775378318702	25.14630118297521	25.43710844238606
29	25.3373663212598	24.7292767099435	24.416032024556948	25.517324944239757
30	25.149891396054358	24.557057461360333	25.20329581560666	25.089755326978658
31	25.787038491399233	24.32582832575652	24.412890602210663	25.474242580633582
32	25.769087506563327	24.817236535639438	23.61766197398005	25.79601398381719
33	25.08829640666161	24.13644543573772	24.846406885997784	25.928851271602888
34	25.647584684151003	24.237528497316315	24.833952645089486	25.280934173443192
35	26.90954458147091	23.7375913260452	24.42152691762256	24.93133717486133
36	24.60765878768024	24.5205965112261	24.749022793262995	26.12272190783067
37	26.273415131088836	24.334682667193235	24.38494610144238	25.006956100275552
38	25.366536671618146	24.051627032388065	24.81678776101854	25.76504853497525
39	26.225726684827244	24.177052152566787	23.771356253954863	25.82586490865111
40	26.192399518911447	24.982946487874056	24.138797637639794	24.685856355574703
41	25.288341575938176	24.380907075475932	24.882643856641504	25.448107491944388
42	26.317773618946323	25.8451927349104	23.784775984094715	24.052257662048568
43	25.80792826593305	23.96296612109163	24.846627143517498	25.38247846945782
44	25.698103384825284	24.594063316249137	23.91907296537982	25.788760333545763
45	26.18354149182999	25.097048382826138	23.85348274670484	24.865927378639032
46	25.165827431761674	24.672608629309494	24.300113992334687	25.861449946594146
47	25.353749770000945	24.295529715878235	25.018512119267417	25.332208394853406
48	26.125572210753074	25.556503006911406	23.63028453460192	24.687640247733597
49	25.23112411589416	24.993717014325206	24.285085987146807	25.49007288263383
50	26.46549325482664	24.919443871002485	23.847306867241706	24.767756006929172
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	5.5
4	10.0
5	11.5
6	13.0
7	12.5
8	12.0
9	21.5
10	31.0
11	49.5
12	68.0
13	118.0
14	168.0
15	229.5
16	291.0
17	380.0
18	469.0
19	499.0
20	529.0
21	669.5
22	810.0
23	974.0
24	1138.0
25	1388.5
26	1639.0
27	2032.0
28	2425.0
29	2767.0
30	3109.0
31	3364.5
32	3620.0
33	4013.5
34	4407.0
35	4967.5
36	5528.0
37	6382.5
38	7237.0
39	7953.5
40	8670.0
41	9504.5
42	10339.0
43	10325.5
44	10312.0
45	11126.0
46	11940.0
47	12739.0
48	13538.0
49	14248.0
50	14958.0
51	14994.5
52	15031.0
53	15044.5
54	15058.0
55	15978.5
56	16899.0
57	16406.5
58	15914.0
59	14781.5
60	13649.0
61	12633.5
62	11618.0
63	10281.0
64	8944.0
65	7854.5
66	6765.0
67	5876.0
68	4987.0
69	4693.0
70	4399.0
71	3446.5
72	2494.0
73	2290.0
74	2086.0
75	1608.5
76	1131.0
77	992.0
78	853.0
79	718.5
80	584.0
81	492.0
82	400.0
83	338.5
84	277.0
85	225.0
86	173.0
87	116.5
88	60.0
89	48.5
90	37.0
91	28.0
92	19.0
93	13.5
94	8.0
95	13.0
96	18.0
97	18.5
98	19.0
99	79.5
100	140.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01795098483590556
2	8.97549241795278E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.48774620897639E-4
17	0.0
18	4.48774620897639E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	4.48774620897639E-4
25	0.0
26	8.97549241795278E-4
27	0.0
28	4.48774620897639E-4
29	0.0
30	4.48774620897639E-4
31	0.0
32	0.0
33	0.0
34	4.48774620897639E-4
35	4.48774620897639E-4
36	0.0
37	0.001346323862692917
38	0.0
39	8.97549241795278E-4
40	4.48774620897639E-4
41	0.001346323862692917
42	0.00448774620897639
43	0.002692647725385834
44	0.004936520829874029
45	8.97549241795278E-4
46	0.0031414223462834733
47	8.97549241795278E-4
48	0.00403897158807875
49	0.002243873104488195
50	0.001346323862692917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	222829.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.82528974664598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6591653014654	62.793110711720686
2	6.840040042839346	9.68895635825515
3	1.730796470750473	3.6775248460012357
4	0.7340113933260702	2.07946278438633
5	0.4451028161586098	1.5762267960740821
6	0.28559415317992964	1.2136373189349907
7	0.2060507246684674	1.0215521594005403
8	0.14549582564417873	0.8243827206541154
9	0.12130640257008483	0.7732404999134586
>10	0.7416386049822189	9.742558648742374
>50	0.06032052531079341	2.9307420164054894
>100	0.03047773910440088	3.6786051395115704
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	395	0.1772659752545674	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	377	0.1691880320784099	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	376	0.16873925745751225	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	302	0.135529935511087	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	267	0.11982282377966962	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	263	0.11802772529607905	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	257	0.11533507757069324	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	256	0.11488630294979559	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	254	0.11398875370800031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.48774620897639E-4
2	0.0	0.0	0.0	0.0	4.48774620897639E-4
3	0.0	0.0	0.0	0.0	4.48774620897639E-4
4	0.0	0.0	0.0	0.0	4.48774620897639E-4
5	0.0	0.0	0.0	0.0	4.48774620897639E-4
6	0.0	0.0	0.0	0.0	4.48774620897639E-4
7	0.0	0.0	0.0	0.0	4.48774620897639E-4
8	0.0	0.0	0.0	0.0	4.48774620897639E-4
9	0.0	0.0	0.0	0.0	4.48774620897639E-4
10	0.0	0.0	0.0	0.0	4.48774620897639E-4
11	0.0	0.0	0.0	0.0	4.48774620897639E-4
12	0.0	0.0	0.0	0.0	8.97549241795278E-4
13	0.0	0.0	0.0	0.0	8.97549241795278E-4
14	0.0	0.0	0.0	0.0	8.97549241795278E-4
15	0.0	0.0	0.0	0.0	8.97549241795278E-4
16	0.0	0.0	0.0	0.0	8.97549241795278E-4
17	0.0	0.0	0.0	0.0	8.97549241795278E-4
18	0.0	0.0	0.0	0.0	8.97549241795278E-4
19	0.0	0.0	0.0	0.0	8.97549241795278E-4
20	0.0	0.0	0.0	0.0	0.001346323862692917
21	0.0	0.0	0.0	0.0	0.001346323862692917
22	0.0	0.0	0.0	0.0	0.001346323862692917
23	0.0	0.0	0.0	0.0	0.001346323862692917
24	0.0	0.0	0.0	0.0	0.001346323862692917
25	0.0	0.0	0.0	0.0	0.001346323862692917
26	0.0	0.0	0.0	4.48774620897639E-4	0.002243873104488195
27	0.0	0.0	0.0	4.48774620897639E-4	0.002243873104488195
28	0.0	0.0	0.0	4.48774620897639E-4	0.002243873104488195
29	0.0	0.0	0.0	4.48774620897639E-4	0.002243873104488195
30	0.0	0.0	0.0	4.48774620897639E-4	0.002243873104488195
31	0.0	0.0	0.0	0.001346323862692917	0.002243873104488195
32	0.0	0.0	0.0	0.001795098483590556	0.002243873104488195
33	0.0	0.0	0.0	0.001795098483590556	0.002243873104488195
34	0.0	0.0	0.0	0.003141422346283473	0.002243873104488195
35	0.0	0.0	0.0	0.003141422346283473	0.002243873104488195
36	0.0	0.0	0.0	0.00448774620897639	0.002243873104488195
37	0.0	0.0	0.0	0.006282844692566946	0.002243873104488195
38	0.0	0.0	0.0	0.009873041659748057	0.002243873104488195
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATGC	25	0.002349512	35.197487	3
GGTAGTG	65	1.7345883E-8	30.459364	16
CTCACGA	30	0.0057370923	29.33124	18
CGAACTG	30	0.0057370923	29.33124	10
GTATCAA	190	0.0	28.945301	1
ATACGAA	70	3.5577614E-8	28.290045	40
TAGTGAC	70	3.565583E-8	28.283695	18
TACGAAT	80	4.269168E-9	27.504208	41
AGTGACG	65	5.898437E-7	27.074991	19
GTGACGA	65	5.898437E-7	27.074991	20
AGGTAGT	75	6.963637E-8	26.398115	15
TAGGGAC	50	8.315524E-5	26.398115	3
GTAGTGA	75	6.963637E-8	26.398115	17
CGAAAAA	70	1.126049E-6	25.141064	24
ACGAAAA	70	1.126049E-6	25.141064	23
TCCCGAC	80	1.3007411E-7	24.748232	2
GGAGGTA	80	1.3007411E-7	24.748232	13
GAGTAAG	45	0.0013952219	24.442698	1
GCAGTTA	45	0.0013952219	24.442698	15
TTCGAGT	45	0.0013952219	24.442698	26
>>END_MODULE