FastQCFastQC Report
Thu 2 Feb 2017
SRR4062274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511702
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28540.18879382312122361No Hit
TATCAACGCAGAGTACTTTTTTTTT18390.12165096030831474No Hit
GCGCAAGACGGACCAGAGCGAAAGC15660.10359184548277371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA853.6379788E-1217.88104416
TTGCGGA400.00528273614.24848619
GGTATCA5950.013.887441
TAAGCCC551.9591078E-413.8190014
GTATCAA17150.012.8482451
GTACCGT604.088716E-412.6678376
GTATTAT905.420279E-712.6636431
ACGAACG1155.326001E-912.39080715
CGTCGTA3000.012.35073210
TAGGACT853.9404895E-612.2948474
GGTTCTA2400.012.27074813
ATAACGA1251.4078978E-912.16112412
TAACGAA1251.4097168E-912.15991513
AACGAAC1251.4097168E-912.15991514
TCATATA550.003071581512.0892242
ATCGCCA2900.011.7922416
CCGTCGT3150.011.7626029
CGCCAGT3000.011.71658518
ACGTTCG907.452383E-611.6121857
TACCGTG907.452383E-611.6121857
Produced by FastQC (version 0.11.5)