##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062274_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1511702
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224806211806296	32.0	32.0	32.0	32.0	32.0
2	30.725077429281697	32.0	32.0	32.0	32.0	32.0
3	30.757025524871967	32.0	32.0	32.0	32.0	32.0
4	30.759991056438373	32.0	32.0	32.0	32.0	32.0
5	30.664396818949765	32.0	32.0	32.0	32.0	32.0
6	34.32278319404221	36.0	36.0	36.0	32.0	36.0
7	34.26021464547907	36.0	36.0	36.0	32.0	36.0
8	34.20881959539645	36.0	36.0	36.0	32.0	36.0
9	34.35434827763673	36.0	36.0	36.0	32.0	36.0
10	34.02604018516877	36.0	36.0	36.0	32.0	36.0
11	34.34187293527428	36.0	36.0	36.0	32.0	36.0
12	34.15343963294353	36.0	36.0	36.0	32.0	36.0
13	34.24653007007995	36.0	36.0	36.0	32.0	36.0
14	34.13627090524455	36.0	36.0	36.0	32.0	36.0
15	34.087287044668855	36.0	36.0	36.0	32.0	36.0
16	34.092534772064866	36.0	36.0	36.0	32.0	36.0
17	34.018810585684214	36.0	36.0	36.0	32.0	36.0
18	34.021674906826874	36.0	36.0	36.0	32.0	36.0
19	34.030503366404226	36.0	36.0	36.0	32.0	36.0
20	34.01684326672849	36.0	36.0	36.0	32.0	36.0
21	34.0093576644074	36.0	36.0	36.0	32.0	36.0
22	33.97359664801661	36.0	36.0	36.0	32.0	36.0
23	33.93305492749232	36.0	36.0	36.0	32.0	36.0
24	33.91133636126697	36.0	36.0	36.0	32.0	36.0
25	33.54736317078366	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	21.0
5	130.0
6	336.0
7	68.0
8	255.0
9	200.0
10	103.0
11	26.0
12	60.0
13	73.0
14	251.0
15	415.0
16	566.0
17	766.0
18	1109.0
19	1500.0
20	2203.0
21	3047.0
22	4583.0
23	6782.0
24	9521.0
25	13545.0
26	18162.0
27	23423.0
28	31706.0
29	41509.0
30	54263.0
31	73616.0
32	104872.0
33	148653.0
34	320253.0
35	649683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59227246928264	16.989242474958424	11.768316965295703	25.650168090463232
2	17.069866421820745	19.122325277717323	36.78529918985195	27.02250911060998
3	19.574967453773777	22.268763031720663	27.741622654290282	30.41464686021528
4	13.247893216613985	15.150316298676984	34.15681692783415	37.444973556874885
5	15.868809109750579	35.640946232460635	32.51926319439482	15.970981463393974
6	36.051935672843655	33.81650410567182	16.011226472233346	14.120333749251182
7	31.678980608273616	29.576107708414867	19.418347541218097	19.326564142093417
8	28.87630806233933	32.50497663003672	18.692527042007658	19.926188265616286
9	27.11606676892347	14.397347151247347	18.126210325817723	40.360375754011464
10	16.90858726495704	26.528160114034087	30.10113106766535	26.462121553343525
11	38.01104277003282	20.985833854999882	21.146341544199235	19.856781830768057
12	24.848066947912546	23.044542962196076	28.074975939103574	24.0324141507878
13	29.683045791252926	19.18177675571993	24.468607928955205	26.66656952407194
14	23.818035517379542	19.218458705996497	24.426393989264653	32.53711178735931
15	25.44453872297413	26.816191973165726	21.54505067526169	26.194218628598453
16	26.565617965175154	25.43719811722506	22.800796609574547	25.196387308025237
17	24.639781381822132	25.708498940621137	24.538314147062586	25.11340553049415
18	25.38268566728433	24.770494560876724	25.351294647764945	24.495525124073996
19	26.105874157731918	24.574429482561342	24.449744670932745	24.869951688773998
20	26.124294264131105	24.149458750992352	24.23579904164926	25.490447943227284
21	26.994748118936702	23.847724582140426	24.040465703865944	25.117061595056928
22	26.35365961062069	23.849201435356957	24.480663140740603	25.316475813281752
23	24.884125087307552	24.127470679558943	25.025075059667056	25.963329173466448
24	25.22126501281539	24.606218793306994	24.833591892434118	25.338924301443495
25	25.228890678051393	24.09714975819822	24.728469577469266	25.945489986281128
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	396.0
1	396.0
2	434.5
3	473.0
4	473.0
5	473.0
6	1007.5
7	1542.0
8	1542.0
9	1542.0
10	1904.5
11	2267.0
12	2267.0
13	2267.0
14	3048.0
15	3829.0
16	3829.0
17	3829.0
18	6013.0
19	8197.0
20	8197.0
21	8197.0
22	12754.0
23	17311.0
24	17311.0
25	17311.0
26	25408.5
27	33506.0
28	33506.0
29	33506.0
30	43122.5
31	52739.0
32	52739.0
33	52739.0
34	66806.0
35	80873.0
36	80873.0
37	80873.0
38	96246.0
39	111619.0
40	111619.0
41	111619.0
42	132768.0
43	153917.0
44	153917.0
45	153917.0
46	171623.0
47	189329.0
48	189329.0
49	189329.0
50	197148.5
51	204968.0
52	204968.0
53	204968.0
54	197983.5
55	190999.0
56	190999.0
57	190999.0
58	178497.5
59	165996.0
60	165996.0
61	165996.0
62	147338.5
63	128681.0
64	128681.0
65	128681.0
66	105163.0
67	81645.0
68	81645.0
69	81645.0
70	62706.0
71	43767.0
72	43767.0
73	43767.0
74	32100.0
75	20433.0
76	20433.0
77	20433.0
78	16102.0
79	11771.0
80	11771.0
81	11771.0
82	7955.5
83	4140.0
84	4140.0
85	4140.0
86	3008.0
87	1876.0
88	1876.0
89	1876.0
90	1254.5
91	633.0
92	633.0
93	633.0
94	407.5
95	182.0
96	182.0
97	182.0
98	397.5
99	613.0
100	613.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010584096601049678
2	1.9845181126968144E-4
3	3.969036225393629E-4
4	0.010319494186023436
5	0.035059819990977056
6	0.0652906459077252
7	0.1074947311044108
8	0.10875159257578543
9	0.11761577347916455
10	0.13084589423047663
11	0.14004082815263855
12	0.13170585207931193
13	0.12528924351492557
14	0.11490359872514555
15	0.13170585207931193
16	0.11926953857307855
17	0.12270936996841969
18	0.1135144360462578
19	0.09882900201230137
20	0.09254469465542811
21	0.09168473680659284
22	0.10286418884145156
23	0.08182829684686532
24	0.09320620069299372
25	0.09089092956151411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1511702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.623466543701426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4293441617362	36.67636192261521
2	13.533786269871959	13.161192078054498
3	4.441352479588253	6.478618611001344
4	2.0137046211374456	3.916531970990942
5	1.1336386484079886	2.756072044675637
6	0.7043246248745519	2.0548022900095697
7	0.494144221176703	1.681890350430417
8	0.34698196527079866	1.349717278368996
9	0.2461256804977707	1.0770735412106192
>10	1.42077382832313	12.852871469577071
>50	0.12835940118088401	4.3527018158633926
>100	0.09764510566210814	9.467674276798657
>500	0.007227869311509112	2.441011093863184
>1k	0.0025911229607296815	1.7334812565405673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2854	0.18879382312122361	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1839	0.12165096030831474	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1566	0.10359184548277371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3230120751312098E-4
2	0.0	0.0	0.0	0.0	1.3230120751312098E-4
3	0.0	0.0	0.0	0.0	1.3230120751312098E-4
4	0.0	0.0	0.0	0.0	1.3230120751312098E-4
5	0.0	0.0	0.0	0.0	1.3230120751312098E-4
6	0.0	0.0	0.0	0.0	1.3230120751312098E-4
7	6.615060375656049E-5	0.0	0.0	0.0	1.3230120751312098E-4
8	6.615060375656049E-5	0.0	0.0	6.615060375656049E-5	1.3230120751312098E-4
9	6.615060375656049E-5	0.0	0.0	6.615060375656049E-5	1.3230120751312098E-4
10	6.615060375656049E-5	0.0	0.0	1.3230120751312098E-4	1.3230120751312098E-4
11	6.615060375656049E-5	0.0	0.0	1.3230120751312098E-4	1.9845181126968147E-4
12	6.615060375656049E-5	0.0	0.0	2.6460241502624196E-4	8.599578488352863E-4
13	6.615060375656049E-5	0.0	0.0	2.6460241502624196E-4	8.599578488352863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	85	3.6379788E-12	17.881044	16
TTGCGGA	40	0.005282736	14.248486	19
GGTATCA	595	0.0	13.88744	1
TAAGCCC	55	1.9591078E-4	13.819001	4
GTATCAA	1715	0.0	12.848245	1
GTACCGT	60	4.088716E-4	12.667837	6
GTATTAT	90	5.420279E-7	12.663643	1
ACGAACG	115	5.326001E-9	12.390807	15
CGTCGTA	300	0.0	12.350732	10
TAGGACT	85	3.9404895E-6	12.294847	4
GGTTCTA	240	0.0	12.270748	13
ATAACGA	125	1.4078978E-9	12.161124	12
TAACGAA	125	1.4097168E-9	12.159915	13
AACGAAC	125	1.4097168E-9	12.159915	14
TCATATA	55	0.0030715815	12.089224	2
ATCGCCA	290	0.0	11.79224	16
CCGTCGT	315	0.0	11.762602	9
CGCCAGT	300	0.0	11.716585	18
ACGTTCG	90	7.452383E-6	11.612185	7
TACCGTG	90	7.452383E-6	11.612185	7
>>END_MODULE