FastQCFastQC Report
Thu 2 Feb 2017
SRR4062274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511702
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15480.10240113461515563No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15440.10213653220012939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.028.0556431
GTATCAA20100.026.933881
TAGGACG1259.968062E-1021.1189844
TCTAGCG3200.020.62469128
TACGAAT3200.019.93851941
GAGCGTA901.0026479E-519.5546157
ATACGAA3400.019.41275640
CAATACG3300.019.33432238
CGCAATA3300.019.33368336
ATAGTAC2200.018.9990863
GTGTAGG2500.018.4858361
ATACCGT5000.018.479116
CTAGCGG3600.018.33305729
CGAATGC3550.017.97334543
AATACGA3550.017.9727539
ATCAACG30050.017.935913
TAGCGGC3700.017.8375730
ATTATAC1852.1827873E-1117.836983
TCAACGC30300.017.7879244
TACCGTC5100.017.6854237
Produced by FastQC (version 0.11.5)