##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1511702
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291740038711332	32.0	32.0	32.0	32.0	32.0
2	31.452228679991162	32.0	32.0	32.0	32.0	32.0
3	31.565968028090193	32.0	32.0	32.0	32.0	32.0
4	31.641966472227992	32.0	32.0	32.0	32.0	32.0
5	31.608610691789785	32.0	32.0	32.0	32.0	32.0
6	35.2478411750464	36.0	36.0	36.0	36.0	36.0
7	35.254187002464775	36.0	36.0	36.0	36.0	36.0
8	35.19994747642062	36.0	36.0	36.0	36.0	36.0
9	35.29234597824174	36.0	36.0	36.0	36.0	36.0
10	35.16898899386255	36.0	36.0	36.0	36.0	36.0
11	35.29018880705324	36.0	36.0	36.0	36.0	36.0
12	35.212670883547155	36.0	36.0	36.0	36.0	36.0
13	35.24584474982503	36.0	36.0	36.0	36.0	36.0
14	35.20474074916882	36.0	36.0	36.0	36.0	36.0
15	35.18786969918674	36.0	36.0	36.0	36.0	36.0
16	35.188422056728115	36.0	36.0	36.0	36.0	36.0
17	35.176999170471426	36.0	36.0	36.0	36.0	36.0
18	35.17130228047591	36.0	36.0	36.0	36.0	36.0
19	35.162235017219004	36.0	36.0	36.0	36.0	36.0
20	35.16111244147325	36.0	36.0	36.0	36.0	36.0
21	35.151185881873545	36.0	36.0	36.0	36.0	36.0
22	35.1366697933852	36.0	36.0	36.0	36.0	36.0
23	35.105465892087196	36.0	36.0	36.0	36.0	36.0
24	35.086009676510315	36.0	36.0	36.0	36.0	36.0
25	35.07141949934577	36.0	36.0	36.0	36.0	36.0
26	35.02709462579265	36.0	36.0	36.0	36.0	36.0
27	35.01770322457733	36.0	36.0	36.0	36.0	36.0
28	34.99876099919164	36.0	36.0	36.0	36.0	36.0
29	34.982835902843284	36.0	36.0	36.0	36.0	36.0
30	34.95496003842027	36.0	36.0	36.0	36.0	36.0
31	34.95544690686392	36.0	36.0	36.0	36.0	36.0
32	34.93248206326379	36.0	36.0	36.0	32.0	36.0
33	34.911940316279264	36.0	36.0	36.0	32.0	36.0
34	34.90576251139444	36.0	36.0	36.0	32.0	36.0
35	34.89086473392243	36.0	36.0	36.0	32.0	36.0
36	34.86640753270155	36.0	36.0	36.0	32.0	36.0
37	34.85648626515014	36.0	36.0	36.0	32.0	36.0
38	34.8318359041663	36.0	36.0	36.0	32.0	36.0
39	34.81669733849661	36.0	36.0	36.0	32.0	36.0
40	34.806828991428205	36.0	36.0	36.0	32.0	36.0
41	34.78753749085468	36.0	36.0	36.0	32.0	36.0
42	34.74295198392276	36.0	36.0	36.0	32.0	36.0
43	34.73655918957572	36.0	36.0	36.0	32.0	36.0
44	34.687302788512554	36.0	36.0	36.0	32.0	36.0
45	34.68061099343654	36.0	36.0	36.0	32.0	36.0
46	34.65727702946745	36.0	36.0	36.0	32.0	36.0
47	34.63444977912314	36.0	36.0	36.0	32.0	36.0
48	34.609320487768095	36.0	36.0	36.0	32.0	36.0
49	34.591589479937184	36.0	36.0	36.0	32.0	36.0
50	34.16818327950879	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	8.0
21	36.0
22	122.0
23	326.0
24	740.0
25	1798.0
26	3641.0
27	7077.0
28	11865.0
29	19001.0
30	28719.0
31	42358.0
32	64633.0
33	109959.0
34	250420.0
35	970997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19682028790251	17.558691535105076	12.16511845506172	26.079369721930696
2	16.492837570899912	19.609390608068864	36.417912225016835	27.47985959601439
3	18.932485192148167	22.650622015772992	28.05719131731338	30.359701474765462
4	12.98980420069842	15.451402096049415	34.18731614254406	37.37147756070811
5	15.501997086727409	35.963437238291675	32.36424903850097	16.170316636479942
6	36.29141780208891	33.93631933341227	15.825571095833233	13.946691768665588
7	31.700956934633943	29.61760981992483	19.38331761153984	19.298115633901393
8	28.96285114394239	32.642147724882285	18.454364682986462	19.94063644818886
9	27.284941079657234	14.152392468886063	18.003812920800527	40.55885353065617
10	16.769001786068667	26.610967784613347	30.159357015280808	26.460673414037178
11	38.240671772611265	20.87653519013668	21.100058080230095	19.78273495702195
12	24.988522870248236	22.974236985860973	28.08820786107315	23.949032282817644
13	29.85588429465596	19.079554039089714	24.50443275195773	26.560128914296598
14	23.83452558771504	19.123742642399097	24.430277925146623	32.61145384473924
15	25.538432839276524	26.813551877287985	21.53294763121303	26.11506765222246
16	26.6408413342912	25.294074065253945	22.81387635328314	25.251208247171714
17	24.78418365524422	25.524144308865104	24.569657247261695	25.122014788628977
18	25.44650033306851	24.60413798760469	25.54995994578293	24.39940173354387
19	26.217336485630106	24.348978833129813	24.508269486975607	24.925415194264477
20	26.270507554744693	23.949145960935343	24.353591554932564	25.426754929387396
21	27.22381792178617	23.70625956703107	24.0791505204068	24.990771990775958
22	26.47836676805349	23.730933742232267	24.579050632995127	25.211648856719115
23	24.97603692793747	23.917560417040143	25.170784434223435	25.935618220798958
24	25.536238652578213	24.386198994897754	24.851771563822407	25.22579078870163
25	25.385195475292715	23.841569094397038	24.981610107825627	25.791625322484617
26	25.086376436547802	24.801795597927363	25.20438950398129	24.90743846154355
27	25.79427345180513	24.165109916279466	24.46827933658619	25.572337295329216
28	25.303504190051306	24.194470243623634	25.00628441544352	25.49574115088154
29	25.24112054571963	24.550702127152885	24.968710557441597	25.239466769685887
30	25.192119288928755	24.495614513812676	24.99677513460092	25.315491062657646
31	25.684695447623657	24.22633151947418	24.25279190100642	25.836181131895742
32	25.69622455412456	23.951421224868344	24.315120371981276	26.037233849025824
33	25.20816010086677	23.84320402832049	24.89447290032328	26.054162970489458
34	25.933871406259613	24.093349765262875	24.842576955211086	25.13020187326643
35	26.2345042005689	23.882979427135016	24.874975193490773	25.00754117880532
36	25.161606138784148	24.577693986902162	24.775550704504862	25.485149169808825
37	26.0326631271028	24.153620423451088	24.383498029337012	25.430218420109096
38	25.404477866669488	24.086890141046315	24.861910614658182	25.646721377626015
39	25.872463775785608	23.861080534710684	24.492953609720473	25.77350207978324
40	26.043941287253613	24.155602404976122	24.93750409307673	24.862952214693536
41	25.310283512008482	24.456598676176604	25.06234776890907	25.17077004290585
42	26.544009028646503	24.381513237670784	24.441051498611106	24.63342623507161
43	25.892851826983186	23.82325215245643	24.506084431199124	25.77781158936126
44	25.238978541215445	24.53596817235827	24.620313539846077	25.604739746580208
45	25.526553762761555	24.57634574764714	24.46448263475668	25.43261785483462
46	25.224751433844688	24.419020024211633	24.689647872882308	25.666580669061368
47	25.641254626459876	24.298183814749766	24.895795089585462	25.164766469204892
48	26.27337455586111	24.67470609599023	23.923664011039715	25.12825533710894
49	25.465845494357197	24.903152825370782	24.152587221994366	25.478414458277655
50	25.700216183891257	24.94860009684615	24.067988113855083	25.28319560540751
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	103.5
2	67.0
3	178.5
4	290.0
5	298.0
6	306.0
7	229.0
8	152.0
9	242.5
10	333.0
11	555.5
12	778.0
13	1467.5
14	2157.0
15	2776.0
16	3395.0
17	3295.5
18	3196.0
19	2875.0
20	2554.0
21	2917.5
22	3281.0
23	4079.5
24	4878.0
25	6084.5
26	7291.0
27	8908.0
28	10525.0
29	12382.0
30	14239.0
31	16424.5
32	18610.0
33	21853.5
34	25097.0
35	29441.5
36	33786.0
37	39959.0
38	46132.0
39	51445.0
40	56758.0
41	62156.0
42	67554.0
43	72058.0
44	76562.0
45	85891.5
46	95221.0
47	102091.5
48	108962.0
49	113140.5
50	117319.0
51	115603.0
52	113887.0
53	115017.0
54	116147.0
55	118420.0
56	120693.0
57	117710.5
58	114728.0
59	106606.5
60	98485.0
61	87457.0
62	76429.0
63	66482.5
64	56536.0
65	47574.5
66	38613.0
67	32784.0
68	26955.0
69	23701.0
70	20447.0
71	15599.0
72	10751.0
73	9373.0
74	7995.0
75	5839.0
76	3683.0
77	3143.0
78	2603.0
79	2176.0
80	1749.0
81	1384.0
82	1019.0
83	877.0
84	735.0
85	566.5
86	398.0
87	274.0
88	150.0
89	95.5
90	41.0
91	32.5
92	24.0
93	20.0
94	16.0
95	16.5
96	17.0
97	13.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032810699463254
2	0.004233638640419871
3	2.6460241502624196E-4
4	6.615060375656049E-5
5	0.0
6	1.9845181126968144E-4
7	0.0
8	0.0
9	0.0
10	1.3230120751312098E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.292048300524839E-4
17	0.0
18	6.615060375656049E-5
19	0.0
20	1.9845181126968144E-4
21	0.0
22	0.0
23	6.615060375656049E-5
24	9.922590563484074E-4
25	1.3230120751312098E-4
26	3.3075301878280244E-4
27	3.969036225393629E-4
28	0.0017199156976705726
29	6.615060375656049E-4
30	7.276566413221653E-4
31	5.292048300524839E-4
32	8.599578488352863E-4
33	3.3075301878280244E-4
34	7.276566413221653E-4
35	1.3230120751312098E-4
36	1.3230120751312098E-4
37	0.0018522169051836935
38	0.0
39	3.969036225393629E-4
40	1.9845181126968144E-4
41	0.0013230120751312098
42	0.0045643916592026735
43	0.003770584414123948
44	0.0039690362253936295
45	0.002182969923966496
46	0.0021168193202099356
47	0.0011245602638615283
48	0.0045643916592026735
49	0.0027783253577755404
50	0.0017199156976705726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1511702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.806411599662695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95322518002601	37.33151184038578
2	14.006576254779091	13.952346040951788
3	4.600799364666917	6.874479205322014
4	1.992801990897806	3.970172647811336
5	1.1266015999951609	2.8055991499098765
6	0.6682494347377134	1.9969863838673094
7	0.4808967703876417	1.6766219738012758
8	0.3320672927887742	1.3231264210738716
9	0.2569508277429404	1.1518018818695028
>10	1.3656929449284079	12.525580464844824
>50	0.12204874062475503	4.238786196598255
>100	0.08448053047742661	8.30456674942627
>500	0.00787409734573175	2.702589173907689
>1k	0.0017349706016019113	1.1458318702302315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1548	0.10240113461515563	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1544	0.10213653220012939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.615060375656049E-5	0.0	0.0	0.0	6.615060375656049E-5
2	6.615060375656049E-5	0.0	0.0	0.0	6.615060375656049E-5
3	6.615060375656049E-5	0.0	0.0	0.0	6.615060375656049E-5
4	6.615060375656049E-5	0.0	0.0	0.0	6.615060375656049E-5
5	6.615060375656049E-5	0.0	0.0	0.0	1.3230120751312098E-4
6	1.3230120751312098E-4	0.0	0.0	0.0	1.9845181126968147E-4
7	1.9845181126968147E-4	0.0	0.0	0.0	1.9845181126968147E-4
8	1.9845181126968147E-4	0.0	0.0	0.0	1.9845181126968147E-4
9	1.9845181126968147E-4	0.0	0.0	0.0	1.9845181126968147E-4
10	1.9845181126968147E-4	0.0	0.0	6.615060375656049E-5	1.9845181126968147E-4
11	1.9845181126968147E-4	0.0	0.0	6.615060375656049E-5	1.9845181126968147E-4
12	1.9845181126968147E-4	0.0	0.0	1.3230120751312098E-4	8.599578488352863E-4
13	1.9845181126968147E-4	0.0	0.0	1.3230120751312098E-4	0.0011907108676180887
14	2.6460241502624196E-4	0.0	0.0	1.3230120751312098E-4	0.0011907108676180887
15	2.6460241502624196E-4	0.0	0.0	2.6460241502624196E-4	0.0012568614713746493
16	2.6460241502624196E-4	0.0	0.0	3.3075301878280244E-4	0.0012568614713746493
17	3.3075301878280244E-4	0.0	0.0	4.630542262959234E-4	0.0013230120751312098
18	3.3075301878280244E-4	0.0	0.0	5.292048300524839E-4	0.0013230120751312098
19	5.292048300524839E-4	0.0	0.0	5.953554338090443E-4	0.0013230120751312098
20	5.292048300524839E-4	0.0	0.0	7.938072450787259E-4	0.0014553132826443306
21	5.292048300524839E-4	0.0	0.0	9.261084525918468E-4	0.0014553132826443306
22	5.953554338090443E-4	0.0	0.0	0.0011245602638615283	0.001521463886400891
23	5.953554338090443E-4	0.0	0.0	0.0013891626788877702	0.001521463886400891
24	6.615060375656049E-4	0.0	0.0	0.002050668716453375	0.001521463886400891
25	6.615060375656049E-4	0.0	0.0	0.0023814217352361774	0.001521463886400891
26	6.615060375656049E-4	0.0	0.0	0.003042927772801782	0.0015876144901574517
27	6.615060375656049E-4	0.0	0.0	0.0035059819990977056	0.0016537650939140122
28	8.599578488352863E-4	0.0	0.0	0.004299789244176432	0.0016537650939140122
29	9.261084525918468E-4	0.0	0.0	0.005159747093011718	0.0016537650939140122
30	9.922590563484074E-4	0.0	0.0	0.007871921847030698	0.0016537650939140122
31	9.922590563484074E-4	0.0	0.0	0.012568614713746493	0.0016537650939140122
32	9.922590563484074E-4	0.0	0.0	0.01772836180675821	0.001786066301427133
33	0.0010584096601049678	0.0	0.0	0.022160452258447764	0.001786066301427133
34	0.0010584096601049678	0.0	0.0	0.026790994521406996	0.001786066301427133
35	0.0012568614713746493	0.0	0.0	0.03287685006701056	0.001786066301427133
36	0.0014553132826443306	0.0	0.0	0.04213793459292903	0.001786066301427133
37	0.0014553132826443306	0.0	0.0	0.05430964568413616	0.001786066301427133
38	0.0014553132826443306	0.0	0.0	0.0709795978307894	0.001786066301427133
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	855	0.0	28.055643	1
GTATCAA	2010	0.0	26.93388	1
TAGGACG	125	9.968062E-10	21.118984	4
TCTAGCG	320	0.0	20.624691	28
TACGAAT	320	0.0	19.938519	41
GAGCGTA	90	1.0026479E-5	19.554615	7
ATACGAA	340	0.0	19.412756	40
CAATACG	330	0.0	19.334322	38
CGCAATA	330	0.0	19.333683	36
ATAGTAC	220	0.0	18.999086	3
GTGTAGG	250	0.0	18.485836	1
ATACCGT	500	0.0	18.47911	6
CTAGCGG	360	0.0	18.333057	29
CGAATGC	355	0.0	17.973345	43
AATACGA	355	0.0	17.97275	39
ATCAACG	3005	0.0	17.93591	3
TAGCGGC	370	0.0	17.83757	30
ATTATAC	185	2.1827873E-11	17.83698	3
TCAACGC	3030	0.0	17.787924	4
TACCGTC	510	0.0	17.685423	7
>>END_MODULE