FastQCFastQC Report
Thu 2 Feb 2017
SRR4062273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193307
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3720.19244000475926892No Hit
TATCAACGCAGAGTACTTTTTTTTT2900.15002043381770966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT250.00601434318.99948310
ACTAGAG350.002161356916.285273
GGTTGAA350.002161356916.285271
GTATCAA2450.015.1220371
GGTATCA602.5460304E-514.2496121
TGGTGTG701.0816733E-412.2139535
TATCAAC3600.010.5552672
ATCAACG3650.010.1504083
CAACGCA3700.010.013245
TCAACGC3700.010.013244
CTGGTGT951.6274223E-49.9997284
AACGCAG3800.09.7497356
TACACTG800.0044863219.4997415
ATGGGAG1000.00288152168.54976755
ACGCAGA4650.08.1718217
CGCAGAG4800.07.71853978
AGAGTAC5050.07.712661311
CAGAGTA5100.07.63704710
GCAGAGT5300.07.1696169
GAGTACA3901.8189894E-127.063909512
Produced by FastQC (version 0.11.5)