##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062273_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193307
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.168441908466843	32.0	32.0	32.0	21.0	32.0
2	29.19055181654053	32.0	32.0	32.0	14.0	32.0
3	29.009446114212107	32.0	32.0	32.0	14.0	32.0
4	29.0548505744748	32.0	32.0	32.0	14.0	32.0
5	28.60099220411056	32.0	32.0	32.0	14.0	32.0
6	32.180510793711555	36.0	32.0	36.0	14.0	36.0
7	31.963710574371337	36.0	32.0	36.0	14.0	36.0
8	31.78690890655796	36.0	32.0	36.0	14.0	36.0
9	32.22970714976695	36.0	32.0	36.0	14.0	36.0
10	31.279477721965577	36.0	32.0	36.0	14.0	36.0
11	32.270771363685746	36.0	32.0	36.0	14.0	36.0
12	31.688759331012328	36.0	32.0	36.0	14.0	36.0
13	31.977657301597976	36.0	32.0	36.0	14.0	36.0
14	31.727676700792003	36.0	32.0	36.0	14.0	36.0
15	31.606832654792637	36.0	32.0	36.0	14.0	36.0
16	31.6365056619781	36.0	32.0	36.0	14.0	36.0
17	31.450371688557578	36.0	32.0	36.0	14.0	36.0
18	31.550761224373666	36.0	32.0	36.0	14.0	36.0
19	31.557263834211902	36.0	32.0	36.0	14.0	36.0
20	31.513369924524202	36.0	32.0	36.0	14.0	36.0
21	31.468751778259453	36.0	32.0	36.0	14.0	36.0
22	31.49861619082599	36.0	32.0	36.0	14.0	36.0
23	31.287620210338993	36.0	32.0	36.0	14.0	36.0
24	31.285975158685407	36.0	32.0	36.0	14.0	36.0
25	30.787757297976793	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	9.0
6	29.0
7	12.0
8	31.0
9	17.0
10	8.0
11	8.0
12	4.0
13	7.0
14	59.0
15	112.0
16	215.0
17	470.0
18	790.0
19	1117.0
20	1482.0
21	2004.0
22	2620.0
23	3113.0
24	4020.0
25	4948.0
26	6424.0
27	7541.0
28	9521.0
29	12173.0
30	14762.0
31	18845.0
32	23438.0
33	26498.0
34	33893.0
35	19132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75316465518579	17.69620344840073	11.439316734520737	26.11131516189274
2	17.2482113943106	19.5000698370985	37.69547921182368	25.556239556767213
3	18.864391172545083	23.47107694536124	29.367427808759167	28.297104073334506
4	13.119706979415097	15.186992038158895	36.34251954246574	35.350781439960265
5	15.096809578508378	35.935446483085485	34.138958740802835	14.828785197603302
6	34.17815202596045	35.195143284493604	16.918283587883053	13.708421101662896
7	29.83063835515722	30.39552234942036	21.22586557728452	18.547973718137904
8	27.913081296014415	33.511436944073395	19.535480931523303	19.040000828388887
9	27.538963392533528	13.753430331900793	18.536736912960183	40.1708693626055
10	15.759684744165334	26.65796709698467	32.56072869252718	25.021619466322825
11	36.18308276322808	21.09708793172202	23.240614626112805	19.479214678937094
12	24.01193139448173	23.277094208302263	29.856968265804955	22.854006131411055
13	29.565555095277546	19.688794531897265	25.51367025683513	25.231980115990062
14	23.16650097344766	19.0164036286815	25.84555320823495	31.971542189635887
15	25.431128235403783	26.62171539839874	22.46579457063253	25.481361795564943
16	25.680804900656078	25.373737164516847	24.111290046966346	24.83416788786073
17	23.952092707756197	25.673275580847438	25.896967217783484	24.47766449361288
18	24.55098501126097	25.12827150586347	26.085583370006987	24.23516011286857
19	25.514995573548976	24.96777233028055	25.315158135609895	24.20207396056058
20	25.494124346430606	24.674638919086814	25.79955479629342	24.03168193818916
21	25.76680764711059	24.172883093217926	25.01824808071605	25.042061178955432
22	25.51256031644128	24.25859962308696	25.322550582972642	24.90628947749912
23	25.077775994782147	24.466438558258233	25.138857974045337	25.316927472914287
24	24.753710519912822	24.674504444340908	25.283304084030917	25.288480951715353
25	25.263888097986737	23.74552852683402	25.6371363935207	25.353446981658546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	17.0
2	50.0
3	83.0
4	83.0
5	83.0
6	183.5
7	284.0
8	284.0
9	284.0
10	327.0
11	370.0
12	370.0
13	370.0
14	537.0
15	704.0
16	704.0
17	704.0
18	1245.5
19	1787.0
20	1787.0
21	1787.0
22	2537.0
23	3287.0
24	3287.0
25	3287.0
26	4645.5
27	6004.0
28	6004.0
29	6004.0
30	7566.0
31	9128.0
32	9128.0
33	9128.0
34	10762.0
35	12396.0
36	12396.0
37	12396.0
38	14169.5
39	15943.0
40	15943.0
41	15943.0
42	17871.5
43	19800.0
44	19800.0
45	19800.0
46	21699.0
47	23598.0
48	23598.0
49	23598.0
50	24440.0
51	25282.0
52	25282.0
53	25282.0
54	23951.5
55	22621.0
56	22621.0
57	22621.0
58	20537.0
59	18453.0
60	18453.0
61	18453.0
62	16149.0
63	13845.0
64	13845.0
65	13845.0
66	11476.0
67	9107.0
68	9107.0
69	9107.0
70	7111.5
71	5116.0
72	5116.0
73	5116.0
74	3810.5
75	2505.0
76	2505.0
77	2505.0
78	1933.0
79	1361.0
80	1361.0
81	1361.0
82	1033.5
83	706.0
84	706.0
85	706.0
86	540.0
87	374.0
88	374.0
89	374.0
90	297.5
91	221.0
92	221.0
93	221.0
94	174.5
95	128.0
96	128.0
97	128.0
98	157.5
99	187.0
100	187.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.17311840750723E-4
4	0.005173118407507229
5	0.021209785470779643
6	0.04655806566756507
7	0.0879430129276229
8	0.0832872063608664
9	0.09156419581287796
10	0.10035849710564025
11	0.1107047339206547
12	0.10501430367239675
13	0.09673731422038519
14	0.09259881949437941
15	0.10811817471690109
16	0.09828924974263735
17	0.09518537869813302
18	0.08380451820161712
19	0.07759677611260844
20	0.07087172218284904
21	0.07035441034209833
22	0.08225258267936494
23	0.06414666825308964
24	0.0724236577051012
25	0.07138903402359977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	193307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.79408005595069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.33551636405647	56.069434604490944
2	12.520689473929448	17.47740006998262
3	3.329387666921569	6.971146480872567
4	1.394720161018783	3.8937284229517353
5	0.7685061665986279	2.6818590457538205
6	0.445636921338411	1.8661691378268275
7	0.3035237108873552	1.482891072358591
8	0.1816224756319726	1.014093888337428
9	0.14526917709198003	0.9125035718057759
>10	0.5574620185641211	6.239569482521308
>50	0.010795805754133787	0.4945590591221362
>100	0.006870058207176046	0.896645163976283
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	372	0.19244000475926892	No Hit
TATCAACGCAGAGTACTTTTTTTTT	290	0.15002043381770966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCTCT	25	0.006014343	18.999483	10
ACTAGAG	35	0.0021613569	16.28527	3
GGTTGAA	35	0.0021613569	16.28527	1
GTATCAA	245	0.0	15.122037	1
GGTATCA	60	2.5460304E-5	14.249612	1
TGGTGTG	70	1.0816733E-4	12.213953	5
TATCAAC	360	0.0	10.555267	2
ATCAACG	365	0.0	10.150408	3
CAACGCA	370	0.0	10.01324	5
TCAACGC	370	0.0	10.01324	4
CTGGTGT	95	1.6274223E-4	9.999728	4
AACGCAG	380	0.0	9.749735	6
TACACTG	80	0.004486321	9.499741	5
ATGGGAG	100	0.0028815216	8.5497675	5
ACGCAGA	465	0.0	8.171821	7
CGCAGAG	480	0.0	7.7185397	8
AGAGTAC	505	0.0	7.7126613	11
CAGAGTA	510	0.0	7.637047	10
GCAGAGT	530	0.0	7.169616	9
GAGTACA	390	1.8189894E-12	7.0639095	12
>>END_MODULE