FastQCFastQC Report
Thu 2 Feb 2017
SRR4062273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193307
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGT250.002347922235.24
TTAGACA300.005733235329.3333324
GTATCAA2100.029.3333321
ATATTAA554.932808E-628.01
GTATTAA407.0106355E-427.5000021
AACATAT508.305895E-526.47
GACTTCT450.001393974124.4444457
GGTATCA852.3314897E-723.2941191
ATTAGAG602.8607066E-422.03
AATTAGA500.00257267622.02
TGGTCGG602.8607066E-422.037
CAACGCA2900.020.482765
TATCAAC3050.020.1967222
TCAACGC3050.020.1967224
TATGCAA550.004470234720.010
ACATATA550.004470234720.08
GCATCGC550.004470234720.014
ATCAACG3250.018.9538463
CAGCACT708.0896553E-418.8571434
AACGCAG3150.018.8571416
Produced by FastQC (version 0.11.5)