##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062273_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193307 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.784787928010886 32.0 32.0 32.0 21.0 32.0 2 28.659541558246726 32.0 32.0 32.0 14.0 32.0 3 30.377663509340064 32.0 32.0 32.0 21.0 32.0 4 30.962639738860982 32.0 32.0 32.0 32.0 32.0 5 30.67829928559235 32.0 32.0 32.0 32.0 32.0 6 34.32390963596766 36.0 36.0 36.0 32.0 36.0 7 34.34903547207292 36.0 36.0 36.0 32.0 36.0 8 34.17840016140129 36.0 36.0 36.0 32.0 36.0 9 34.58360018002452 36.0 36.0 36.0 32.0 36.0 10 34.00652330231187 36.0 36.0 36.0 32.0 36.0 11 34.66355072501254 36.0 36.0 36.0 32.0 36.0 12 34.402546208880175 36.0 36.0 36.0 32.0 36.0 13 34.58894918445788 36.0 36.0 36.0 32.0 36.0 14 34.40137708412008 36.0 36.0 36.0 32.0 36.0 15 34.310392277568845 36.0 36.0 36.0 32.0 36.0 16 34.36974863817658 36.0 36.0 36.0 32.0 36.0 17 34.326847967223124 36.0 36.0 36.0 32.0 36.0 18 34.32640307904008 36.0 36.0 36.0 32.0 36.0 19 34.359505863729716 36.0 36.0 36.0 32.0 36.0 20 34.37545458778006 36.0 36.0 36.0 32.0 36.0 21 34.34584365801549 36.0 36.0 36.0 32.0 36.0 22 34.331281329698356 36.0 36.0 36.0 32.0 36.0 23 34.24365387699359 36.0 36.0 36.0 32.0 36.0 24 34.20525899217307 36.0 36.0 36.0 32.0 36.0 25 34.19459202201679 36.0 36.0 36.0 32.0 36.0 26 34.09823751855856 36.0 36.0 36.0 32.0 36.0 27 34.057571634757146 36.0 36.0 36.0 32.0 36.0 28 34.06046858106535 36.0 36.0 36.0 32.0 36.0 29 33.97690719942889 36.0 36.0 36.0 32.0 36.0 30 33.943706125489506 36.0 36.0 36.0 32.0 36.0 31 33.97583636391853 36.0 36.0 36.0 32.0 36.0 32 33.93637064358766 36.0 36.0 36.0 32.0 36.0 33 33.88698288215119 36.0 36.0 36.0 32.0 36.0 34 33.86434531599994 36.0 36.0 36.0 32.0 36.0 35 33.82671605270373 36.0 36.0 36.0 32.0 36.0 36 33.74860196475037 36.0 36.0 36.0 32.0 36.0 37 33.76315394683069 36.0 36.0 36.0 32.0 36.0 38 33.75214037774111 36.0 36.0 36.0 32.0 36.0 39 33.69815888715877 36.0 36.0 36.0 32.0 36.0 40 33.68863000305214 36.0 36.0 36.0 32.0 36.0 41 33.61796520560559 36.0 36.0 36.0 27.0 36.0 42 33.5908218533214 36.0 36.0 36.0 27.0 36.0 43 33.588695701655915 36.0 36.0 36.0 27.0 36.0 44 33.48600412814849 36.0 36.0 36.0 21.0 36.0 45 33.48674388408076 36.0 36.0 36.0 21.0 36.0 46 33.470908968635385 36.0 36.0 36.0 21.0 36.0 47 33.42321799003658 36.0 36.0 36.0 21.0 36.0 48 33.3706591070163 36.0 36.0 36.0 21.0 36.0 49 33.334007563099114 36.0 36.0 36.0 21.0 36.0 50 32.639811284640494 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 3.0 22 11.0 23 50.0 24 107.0 25 294.0 26 655.0 27 1302.0 28 2582.0 29 4686.0 30 8056.0 31 13632.0 32 23046.0 33 37548.0 34 57396.0 35 43937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.293263036988364 16.960114240183778 11.651153525768715 24.095469197059145 2 17.398591848031327 20.440448415182384 37.48363967450065 24.677320062285634 3 19.9651331819334 24.37418200065181 29.463495889957425 26.19718892745736 4 14.772874236318396 17.142162466956705 36.613780152813916 31.47118314391098 5 17.60360462890635 35.06753506081001 31.996254662272968 15.332605648010677 6 34.29363654704692 36.54652961351633 16.20117222863114 12.95866161080561 7 30.143243648703876 30.881447645455157 20.337597707273922 18.637710998567048 8 28.176941342010377 33.473697279457035 18.81256240074079 19.536798977791804 9 27.26854174965211 13.971040883154776 17.520834734386234 41.23958263280688 10 15.389509950493258 27.089551852752358 32.10644208435287 25.41449611240152 11 36.59515692654689 21.0013087989571 22.62773722627737 19.77579704821864 12 23.775652200903227 23.174535841950885 29.682836110435733 23.366975846710154 13 29.73767116555531 19.795972210007914 25.01099287661595 25.455363747820826 14 23.264548102241513 18.84101455198208 25.487437081947366 32.40700026382904 15 25.063241372531774 27.008851205595246 22.14715452621995 25.78075289565303 16 25.312847572489073 25.766534750782444 23.697783295827836 25.22283438090065 17 24.075175756697895 25.65866730123586 25.192569332719454 25.07358760934679 18 24.369526194085054 25.47864278065461 25.70367343138117 24.448157593879166 19 25.4062191229495 25.317758798181135 25.033754597608986 24.24226748126038 20 25.658149989395106 24.668015126198224 25.117041803969855 24.55679308043682 21 25.921461716337223 24.535583294966038 24.63128598550492 24.911669003191815 22 25.422255790012777 24.580589425111352 24.945811584681362 25.05134320019451 23 24.989783091145174 24.502475337157993 24.65922082490546 25.848520746791376 24 24.60140916474398 25.0763038912398 25.02146855245052 25.300818391565706 25 24.94995007940737 24.361249204633044 25.25206019440579 25.436740521553798 26 24.879078357224518 24.82217405474194 25.131526535510872 25.16722105252267 27 24.693880718235757 25.1889481498342 25.07358760934679 25.043583522583248 28 24.928869253929005 24.728668535896453 25.04888622184516 25.293575988329387 29 24.797860398226653 25.18377503142669 24.860455130957494 25.157909439389158 30 24.883862890960444 25.099583044499397 24.84868550381261 25.167868560727552 31 24.985644596419167 24.739921472062573 24.903909325580553 25.370524605937707 32 25.0219858669674 25.205632520459787 24.83678726992437 24.935594342648443 33 24.988748467463672 24.756475450966597 25.09479739481757 25.15997868675216 34 25.000646639800937 24.968055993833644 25.278443098284075 24.752854268081343 35 25.40376398042482 24.570887608248064 25.062853713800916 24.962494697526203 36 24.899253519013797 25.08134728695805 24.80872394688242 25.21067524714573 37 25.41759998344602 24.515925445017512 25.017200618704962 25.04927395283151 38 25.329788004510984 24.725047334278294 25.025607068585558 24.919557592625164 39 25.14239008416664 25.171876859089426 24.582141360633603 25.103591696110332 40 25.43622320971305 24.962882875426136 24.955123197814874 24.64577071704594 41 24.688835318096697 24.949561834604204 25.303404964150104 25.058197883148996 42 25.114977314936965 25.19930263477825 25.02599599584064 24.65972405444415 43 24.888256595964823 24.513191929643042 25.333160889808585 25.265390584583546 44 24.855150436617972 24.871704672433058 25.044489508753053 25.228655382195917 45 25.11820636710707 24.949561834604204 24.861618366734607 25.07061343155412 46 25.1175591158106 24.93287878866259 24.769925558826117 25.179636536700688 47 25.17420656475518 25.21973047774243 24.86381624893303 24.74224670856936 48 25.032203328453257 25.28310476298868 24.56247445719932 25.122217451358747 49 25.207971194437544 24.98085837265127 24.647173247247743 25.16399718566344 50 25.27378729171732 24.85838588359449 24.60076458690063 25.267062237787563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 2.5 2 5.0 3 10.5 4 16.0 5 18.5 6 21.0 7 22.0 8 23.0 9 28.0 10 33.0 11 39.0 12 45.0 13 84.5 14 124.0 15 177.5 16 231.0 17 281.5 18 332.0 19 364.0 20 396.0 21 519.0 22 642.0 23 792.5 24 943.0 25 1180.5 26 1418.0 27 1857.5 28 2297.0 29 2663.5 30 3030.0 31 3306.0 32 3582.0 33 4099.0 34 4616.0 35 5254.5 36 5893.0 37 6560.5 38 7228.0 39 7832.5 40 8437.0 41 9028.0 42 9619.0 43 10005.5 44 10392.0 45 11013.5 46 11635.0 47 12126.5 48 12618.0 49 12955.0 50 13292.0 51 13551.5 52 13811.0 53 13854.5 54 13898.0 55 13854.0 56 13810.0 57 13407.0 58 13004.0 59 12127.5 60 11251.0 61 10303.0 62 9355.0 63 8130.0 64 6905.0 65 5899.0 66 4893.0 67 4186.5 68 3480.0 69 2829.0 70 2178.0 71 1793.0 72 1408.0 73 1151.0 74 894.0 75 735.0 76 576.0 77 489.0 78 402.0 79 328.0 80 254.0 81 206.5 82 159.0 83 115.5 84 72.0 85 58.0 86 44.0 87 30.5 88 17.0 89 10.5 90 4.0 91 7.0 92 10.0 93 7.0 94 4.0 95 4.0 96 4.0 97 3.0 98 2.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015519355222521689 2 0.002069247363002892 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.001034623681501446 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 5.17311840750723E-4 25 0.0 26 0.0 27 0.0 28 5.17311840750723E-4 29 0.0 30 5.17311840750723E-4 31 0.0 32 5.17311840750723E-4 33 0.0 34 0.0 35 5.17311840750723E-4 36 0.0 37 0.0 38 5.17311840750723E-4 39 0.0 40 0.0 41 5.17311840750723E-4 42 0.004138494726005784 43 0.003621182885255061 44 0.0015519355222521688 45 5.17311840750723E-4 46 0.0 47 0.001034623681501446 48 0.002069247363002892 49 0.005690430248257952 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 193307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.01580105478438 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18248104137164 53.95826557520756 2 14.041850326678379 19.38219097174188 3 3.725523160046621 7.713598957163077 4 1.5615973096450695 4.310995570006025 5 0.7826135184209551 2.7006349445062727 6 0.49481925630589724 2.04902084107705 7 0.30294126256324455 1.4635413735845062 8 0.19891783976723645 1.0982779244498453 9 0.14597676628187212 0.9067233114297399 >10 0.5564222658519141 6.093582369639368 >50 0.005877645486132437 0.24000982682870176 >100 9.796075810220729E-4 0.08315833436600088 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 5.17311840750723E-4 0.0 35 0.0 0.0 0.0 5.17311840750723E-4 0.0 36 0.0 0.0 0.0 5.17311840750723E-4 0.0 37 0.0 0.0 0.0 5.17311840750723E-4 0.0 38 0.0 0.0 0.0 0.001034623681501446 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTAGT 25 0.0023479222 35.2 4 TTAGACA 30 0.0057332353 29.333332 4 GTATCAA 210 0.0 29.333332 1 ATATTAA 55 4.932808E-6 28.0 1 GTATTAA 40 7.0106355E-4 27.500002 1 AACATAT 50 8.305895E-5 26.4 7 GACTTCT 45 0.0013939741 24.444445 7 GGTATCA 85 2.3314897E-7 23.294119 1 ATTAGAG 60 2.8607066E-4 22.0 3 AATTAGA 50 0.002572676 22.0 2 TGGTCGG 60 2.8607066E-4 22.0 37 CAACGCA 290 0.0 20.48276 5 TATCAAC 305 0.0 20.196722 2 TCAACGC 305 0.0 20.196722 4 TATGCAA 55 0.0044702347 20.0 10 ACATATA 55 0.0044702347 20.0 8 GCATCGC 55 0.0044702347 20.0 14 ATCAACG 325 0.0 18.953846 3 CAGCACT 70 8.0896553E-4 18.857143 4 AACGCAG 315 0.0 18.857141 6 >>END_MODULE