FastQCFastQC Report
Thu 2 Feb 2017
SRR4062272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361555
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21050.5822074096610474No Hit
TATCAACGCAGAGTACTTTTTTTTT13250.366472597530113No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8950.2475418677656235No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8700.24062729045373457No Hit
GGTATCAACGCAGAGTACTTTTTTT7900.21850064305569003No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6410.17728976227683202No Hit
GTACATGGGGTGGTATCAACGCAAA6300.1742473482596009No Hit
GTATCAACGCAGAGTACATGGGGTG4680.12944088727856065No Hit
TATCAACGCAGAGTACATGGGGTGG3780.10454840895576054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGC350.002163120316.2888016
AAGACGG508.6704014E-515.2028825
CCCTACA456.7561236E-414.7744392
AGAACCG400.00526209514.2527025
CGCAAGA551.9573497E-413.815062
ACTGCTC707.211529E-613.5740018
ACGGACC500.001493524913.3025228
GACGGAC500.001493524913.3025227
GCGAAAG500.001498211113.29699518
CAAGACG655.420248E-513.156344
AGGACTG604.0719975E-412.6690685
TAGCTGA604.0719975E-412.6690686
CGGACCA604.0719975E-412.6690689
GTGTATA751.4745767E-512.6638051
GTCCTGA1001.4347279E-712.347211
TAGAACA853.9113766E-612.2964494
TTTACAC550.00305626312.0932023
GCATTCG550.00305626312.0932028
TACTGCT550.00305626312.0932027
CTTACAC550.00305626312.0932023
Produced by FastQC (version 0.11.5)