##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062272_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361555
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07408278131958	32.0	32.0	32.0	32.0	32.0
2	30.82030396481863	32.0	32.0	32.0	32.0	32.0
3	30.853242245301544	32.0	32.0	32.0	32.0	32.0
4	30.80978274398086	32.0	32.0	32.0	32.0	32.0
5	30.838218251718274	32.0	32.0	32.0	32.0	32.0
6	34.40810111877861	36.0	36.0	36.0	32.0	36.0
7	34.323884332950726	36.0	36.0	36.0	32.0	36.0
8	34.222997884139346	36.0	36.0	36.0	32.0	36.0
9	34.27779729225153	36.0	36.0	36.0	32.0	36.0
10	34.13359239949662	36.0	36.0	36.0	32.0	36.0
11	34.370731977154236	36.0	36.0	36.0	32.0	36.0
12	34.23356335827191	36.0	36.0	36.0	32.0	36.0
13	34.31045622381104	36.0	36.0	36.0	32.0	36.0
14	34.228106373857365	36.0	36.0	36.0	32.0	36.0
15	34.23445395582968	36.0	36.0	36.0	32.0	36.0
16	34.24142384976006	36.0	36.0	36.0	32.0	36.0
17	34.154009763383165	36.0	36.0	36.0	32.0	36.0
18	34.198122000802094	36.0	36.0	36.0	32.0	36.0
19	34.15564990112154	36.0	36.0	36.0	32.0	36.0
20	34.07948721494655	36.0	36.0	36.0	32.0	36.0
21	34.0630360526061	36.0	36.0	36.0	32.0	36.0
22	34.05754864405139	36.0	36.0	36.0	32.0	36.0
23	34.074447871001645	36.0	36.0	36.0	32.0	36.0
24	34.05818755099501	36.0	36.0	36.0	32.0	36.0
25	33.75456569539904	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	10.0
5	24.0
6	72.0
7	26.0
8	59.0
9	52.0
10	25.0
11	9.0
12	11.0
13	14.0
14	130.0
15	152.0
16	203.0
17	256.0
18	349.0
19	540.0
20	722.0
21	1042.0
22	1410.0
23	1905.0
24	2415.0
25	3139.0
26	4079.0
27	5120.0
28	6531.0
29	8672.0
30	11299.0
31	15264.0
32	21774.0
33	31964.0
34	72342.0
35	171945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.079551269108535	17.11505643709221	11.794398055056934	25.010994238742313
2	16.546030341165245	19.233864833842706	37.84043921395085	26.379665611041197
3	19.91680357567611	23.045796755118182	27.534199593974897	29.503200075230808
4	12.89904983195718	15.068531042971939	34.112277498858965	37.920141626211915
5	14.7939308085615	36.58281502467961	32.57403882334713	16.049215343411763
6	35.11548414774221	34.11439088858444	16.339380855509887	14.430744108163463
7	30.85259255362448	30.573775711647457	19.239469611179256	19.334162123548808
8	28.68059939528857	32.78621346535092	18.545868358972655	19.98731878038786
9	27.268976326494442	14.634983841980942	18.584906444100454	39.51113338742416
10	16.70936135212453	27.26708779781274	30.233929951175742	25.789620898886984
11	36.50428323348815	21.859585275536688	21.686206409478785	19.949925081496374
12	25.192820566788615	23.839407123491014	28.101260914959553	22.86651139476082
13	29.662861605436753	20.00426477022858	25.056909758569695	25.27596386576497
14	23.792382561021196	19.50906145730939	25.615473009456036	31.083082972213376
15	24.798586435429954	27.273432242233564	22.901186186726783	25.026795135609696
16	24.98241610961271	26.25842503724462	24.308690041702896	24.450468811439773
17	23.483849885907972	26.32119674782339	25.23039943286293	24.964553933405703
18	23.94049141926802	25.57301790352145	26.857497272661828	23.6289934045487
19	25.172687783256414	25.00906697379022	26.249096763297995	23.56914847965537
20	25.616508133367294	24.464050405837863	25.905522274020843	24.013919186774004
21	26.253837572079913	24.17898796061246	25.65560541592499	23.911569051382635
22	25.69296788482835	24.214839424141747	25.938538205980066	24.153654485049834
23	24.26329338168129	24.625626262905858	26.53251031084784	24.578570044565005
24	24.283385052332616	24.877438428554424	26.45530620323491	24.383870315878056
25	24.276507748440068	25.056611503900516	26.376808384592813	24.2900723630666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	226.0
1	226.0
2	301.0
3	376.0
4	376.0
5	376.0
6	808.5
7	1241.0
8	1241.0
9	1241.0
10	1241.5
11	1242.0
12	1242.0
13	1242.0
14	1173.5
15	1105.0
16	1105.0
17	1105.0
18	1758.0
19	2411.0
20	2411.0
21	2411.0
22	3864.0
23	5317.0
24	5317.0
25	5317.0
26	7524.5
27	9732.0
28	9732.0
29	9732.0
30	12457.5
31	15183.0
32	15183.0
33	15183.0
34	17829.0
35	20475.0
36	20475.0
37	20475.0
38	24304.5
39	28134.0
40	28134.0
41	28134.0
42	32509.0
43	36884.0
44	36884.0
45	36884.0
46	41698.0
47	46512.0
48	46512.0
49	46512.0
50	49084.0
51	51656.0
52	51656.0
53	51656.0
54	48021.5
55	44387.0
56	44387.0
57	44387.0
58	40751.5
59	37116.0
60	37116.0
61	37116.0
62	31925.0
63	26734.0
64	26734.0
65	26734.0
66	21638.5
67	16543.0
68	16543.0
69	16543.0
70	12479.5
71	8416.0
72	8416.0
73	8416.0
74	6298.0
75	4180.0
76	4180.0
77	4180.0
78	3169.0
79	2158.0
80	2158.0
81	2158.0
82	1492.5
83	827.0
84	827.0
85	827.0
86	613.5
87	400.0
88	400.0
89	400.0
90	256.0
91	112.0
92	112.0
93	112.0
94	75.0
95	38.0
96	38.0
97	38.0
98	94.0
99	150.0
100	150.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.531661849511139E-4
2	0.0
3	2.7658309247555695E-4
4	0.01106332369902228
5	0.03401972037449351
6	0.06914577311888924
7	0.10676107369556498
8	0.10814398915794277
9	0.12003706213439172
10	0.12833455490865844
11	0.13718521386787624
12	0.12944088727856065
13	0.12639847326132955
14	0.11339906791497836
15	0.13331305057321846
16	0.11865414667201395
17	0.12252630996667174
18	0.1098034877127961
19	0.09791041473634716
20	0.09044267123950712
21	0.08961292196208046
22	0.09818699782882272
23	0.07882618135553374
24	0.08518759248247156
25	0.08878317268465379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	361555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.5140938540536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83601895725293	58.27784890144697
2	10.566723693522759	14.69072445122786
3	2.6766651485762707	5.581978570620157
4	1.0091140961300586	2.805906079513335
5	0.5549613450715817	1.9288817513338878
6	0.3069726653012538	1.2803355999828205
7	0.19922352654038294	0.9694190045304604
8	0.14737503868903834	0.8195713816939674
9	0.10569408697200329	0.6612505813431307
>10	0.5333218435307324	6.68497994125274
>50	0.04195379895878922	2.0817400462831688
>100	0.01917887952366831	2.2020060155714187
>500	0.0019977999503821156	1.0626734378880232
>1k	7.991199801528463E-4	0.9526842373120542
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2105	0.5822074096610474	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1325	0.366472597530113	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	895	0.2475418677656235	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	870	0.24062729045373457	No Hit
GGTATCAACGCAGAGTACTTTTTTT	790	0.21850064305569003	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	641	0.17728976227683202	No Hit
GTACATGGGGTGGTATCAACGCAAA	630	0.1742473482596009	No Hit
GTATCAACGCAGAGTACATGGGGTG	468	0.12944088727856065	No Hit
TATCAACGCAGAGTACATGGGGTGG	378	0.10454840895576054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCGC	35	0.0021631203	16.288801	6
AAGACGG	50	8.6704014E-5	15.202882	5
CCCTACA	45	6.7561236E-4	14.774439	2
AGAACCG	40	0.005262095	14.252702	5
CGCAAGA	55	1.9573497E-4	13.81506	2
ACTGCTC	70	7.211529E-6	13.574001	8
ACGGACC	50	0.0014935249	13.302522	8
GACGGAC	50	0.0014935249	13.302522	7
GCGAAAG	50	0.0014982111	13.296995	18
CAAGACG	65	5.420248E-5	13.15634	4
AGGACTG	60	4.0719975E-4	12.669068	5
TAGCTGA	60	4.0719975E-4	12.669068	6
CGGACCA	60	4.0719975E-4	12.669068	9
GTGTATA	75	1.4745767E-5	12.663805	1
GTCCTGA	100	1.4347279E-7	12.34721	1
TAGAACA	85	3.9113766E-6	12.296449	4
TTTACAC	55	0.003056263	12.093202	3
GCATTCG	55	0.003056263	12.093202	8
TACTGCT	55	0.003056263	12.093202	7
CTTACAC	55	0.003056263	12.093202	3
>>END_MODULE