FastQCFastQC Report
Thu 2 Feb 2017
SRR4062272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361555
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.2320532145869923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.15129095158412964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.12888772109360955No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.10454840895576054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT353.2144447E-431.4268916
TCGGCGA300.00573737529.33582313
GGACCGT407.024733E-427.498536
GTACATA508.320753E-526.4022431
GTCCTAC801.3034514E-724.75211
GTGCGGG450.001395630924.446521
ACCGTCG450.001396756224.4431388
CGACGGT752.0585703E-623.4654147
GACGGTA752.0585703E-623.4654148
GTATTAC500.002575702522.001871
TAACGCC602.8684238E-421.9988274
CGTTTAT500.002577772421.99882531
ATTCCGA703.2086467E-521.99882343
ACGACGG803.6056226E-621.9988236
ACGGTAT803.6056226E-621.9988239
TTCGTTC755.461295E-520.53223640
AATTCCG755.461295E-520.53223642
AGCGATT654.9201556E-420.30941626
GTAGGAC1301.6134436E-920.3066083
CACCCGT550.004475441320.00169828
Produced by FastQC (version 0.11.5)