FastQCFastQC Report
Thu 2 Feb 2017
SRR4062271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242075
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11280.46597128988949704No Hit
TATCAACGCAGAGTACTTTTTTTTT7200.29742848290818963No Hit
GGTATCAACGCAGAGTACTTTTTTT4400.1817618506661159No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT4290.17721780439946297No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3200.13219043684808426No Hit
ACCATACTCCCCCCGGAACCCAAAG3180.13136424661778376No Hit
CTCCACTCCTGGTGGTGCCCTTCCG2930.12103686873902715No Hit
TCGTAGTTCCGACCATAAACGATGC2850.11773210781782506No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG2800.11566663224207374No Hit
GAATAGGACCGCGGTTCTATTTTGT2660.10988330062997005No Hit
GTCAATCCTGTCCGTGTCCGGGCCG2480.10244758855726531No Hit
GTATCTGATCGTCTTCGAACCTCCG2460.10162139832696478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACC402.7488108E-416.6251813
CCGACCC350.002162923816.285894
GGTATCA1050.016.2825241
TCTTGCG400.005261683414.2501552
CCAAGAC400.005261683414.2501553
CGGTCCA400.005261683414.25015510
CGCCGGT400.005261683414.2501557
GCCGGTC500.001493048413.3001448
GGTATCT500.001493048413.30014411
CAAGACG500.001493048413.3001444
ACGGTAT500.001493048413.3001449
GTATCAA3400.013.1297811
CCGTCAA701.0834633E-412.21441818
TATTCCA550.00305533312.0910425
GACGGTA550.00305533312.0910428
CAAGACC550.00305533312.0910424
GGACAGG550.00305533312.0910426
GTCTTGC550.00306007112.0885411
TACGACG657.979789E-411.6924355
GAACTAC657.993653E-411.6900171