##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062271_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242075
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.948363110606216	32.0	32.0	32.0	14.0	32.0
2	25.94842507487349	32.0	14.0	32.0	14.0	32.0
3	26.299568315604667	32.0	21.0	32.0	14.0	32.0
4	26.05679644738201	32.0	21.0	32.0	14.0	32.0
5	26.355534441805226	32.0	21.0	32.0	14.0	32.0
6	29.538618196839824	36.0	21.0	36.0	14.0	36.0
7	29.365374367448105	36.0	21.0	36.0	14.0	36.0
8	29.171645151296087	32.0	21.0	36.0	14.0	36.0
9	29.352865847361354	32.0	21.0	36.0	14.0	36.0
10	28.19850872663431	32.0	21.0	36.0	14.0	36.0
11	30.032890633068263	36.0	21.0	36.0	14.0	36.0
12	28.877632964990188	32.0	21.0	36.0	14.0	36.0
13	29.361363213879997	36.0	21.0	36.0	14.0	36.0
14	28.81996075596406	32.0	21.0	36.0	14.0	36.0
15	28.732322627284933	32.0	21.0	36.0	14.0	36.0
16	28.86774346793349	32.0	21.0	36.0	14.0	36.0
17	28.626487658783436	32.0	21.0	36.0	14.0	36.0
18	28.72968294949912	32.0	21.0	36.0	14.0	36.0
19	28.66416193328514	32.0	21.0	36.0	14.0	36.0
20	28.657469792419704	32.0	21.0	36.0	14.0	36.0
21	28.607551378704947	32.0	21.0	36.0	14.0	36.0
22	28.412648972425902	32.0	21.0	36.0	14.0	36.0
23	28.30463286171641	32.0	21.0	36.0	14.0	36.0
24	28.23667045337189	32.0	21.0	36.0	14.0	36.0
25	27.340192089228545	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	25.0
5	34.0
6	42.0
7	19.0
8	28.0
9	24.0
10	10.0
11	5.0
12	11.0
13	11.0
14	284.0
15	616.0
16	1274.0
17	2500.0
18	4383.0
19	6610.0
20	8467.0
21	10175.0
22	10937.0
23	11432.0
24	11793.0
25	11832.0
26	12813.0
27	12884.0
28	13592.0
29	14228.0
30	14576.0
31	16263.0
32	18460.0
33	18776.0
34	23065.0
35	16902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65151152127036	19.633665738575807	12.532531374703604	28.182291365450236
2	15.942166683878964	19.700506041516057	37.983269647836416	26.374057626768565
3	17.72549829598265	23.206444283796344	28.42507487348962	30.642982546731385
4	12.343781630992073	15.702605670752618	34.78427921619176	37.16933348206355
5	15.00415301269045	35.81219291467109	32.92037372981863	16.263280342819833
6	33.93598948451819	35.5686999078242	16.113818517002922	14.381492090654696
7	29.475468811379656	30.54437943225753	20.512332788884986	19.467818967477825
8	27.70700241890467	32.29258409311749	19.23049887324526	20.769914614732578
9	26.042799545125604	13.966297942727179	18.830145766566734	41.16075674558048
10	15.870619812323458	26.1564357172693	30.77018515378476	27.202759316622483
11	36.10487263161813	21.236749262756486	22.283986615876483	20.374391489748902
12	23.910553067242358	23.625603295326243	28.62152963022701	23.84231400720439
13	28.241989114790982	19.4548296911446	25.31927740740128	26.983903786663138
14	22.95067053175256	19.142099800267136	25.51370217059585	32.39352749738445
15	24.331042126769066	26.95021381837432	22.306177986219673	26.412566068636938
16	25.142774435001964	26.025267249757043	23.69414635154974	25.137811963691252
17	22.944433646653074	26.521075680571006	25.70393558818786	24.83055508458806
18	23.868833478063102	25.095728404250924	26.65550179878427	24.379936318901706
19	24.34331030319569	25.264102341466238	26.000074424143193	24.39251293119488
20	24.780253855376856	24.074916277339067	25.521561169223133	25.62326869806094
21	25.399398008798336	24.148281678960075	25.632586908345186	24.819733403896404
22	24.627331632463186	24.269239198302987	26.008013662094715	25.09541550713911
23	23.37325687637207	24.234017835363964	26.65671678814614	25.73600850011783
24	23.47736565963939	25.085274138266072	26.411622916726962	25.025737285367576
25	23.576859312791214	24.304501874919897	26.357611513289815	25.76102729899908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	21.0
2	61.5
3	102.0
4	102.0
5	102.0
6	272.0
7	442.0
8	442.0
9	442.0
10	522.5
11	603.0
12	603.0
13	603.0
14	773.0
15	943.0
16	943.0
17	943.0
18	1614.0
19	2285.0
20	2285.0
21	2285.0
22	3342.0
23	4399.0
24	4399.0
25	4399.0
26	6275.5
27	8152.0
28	8152.0
29	8152.0
30	9719.5
31	11287.0
32	11287.0
33	11287.0
34	13523.5
35	15760.0
36	15760.0
37	15760.0
38	17695.0
39	19630.0
40	19630.0
41	19630.0
42	22019.5
43	24409.0
44	24409.0
45	24409.0
46	26758.5
47	29108.0
48	29108.0
49	29108.0
50	30110.0
51	31112.0
52	31112.0
53	31112.0
54	29053.0
55	26994.0
56	26994.0
57	26994.0
58	25084.5
59	23175.0
60	23175.0
61	23175.0
62	20361.5
63	17548.0
64	17548.0
65	17548.0
66	14844.0
67	12140.0
68	12140.0
69	12140.0
70	9305.5
71	6471.0
72	6471.0
73	6471.0
74	4931.5
75	3392.0
76	3392.0
77	3392.0
78	2844.5
79	2297.0
80	2297.0
81	2297.0
82	1629.0
83	961.0
84	961.0
85	961.0
86	717.0
87	473.0
88	473.0
89	473.0
90	312.5
91	152.0
92	152.0
93	152.0
94	95.5
95	39.0
96	39.0
97	39.0
98	109.5
99	180.0
100	180.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.130951151502634E-4
2	0.0
3	0.0
4	0.010740472993906848
5	0.03387379944232159
6	0.06031188681193845
7	0.0991428276360632
8	0.09501187648456057
9	0.10327377878756583
10	0.11401425178147269
11	0.12227615408447795
12	0.11484044201177321
13	0.11525353712692346
14	0.10409996901786638
15	0.11607972735722401
16	0.10740472993906847
17	0.10575234947846743
18	0.10120830321181451
19	0.09005473510275741
20	0.08468449860580399
21	0.08716306929670557
22	0.09831663740576269
23	0.08096664256945162
24	0.08592378395125479
25	0.08179283279975214
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	242075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.56851240415247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79576409100765	60.20024939106302
2	7.499744249543183	10.284926132055459
3	1.9193291089735558	3.948166254489125
4	0.8435889586182491	2.3137455989208595
5	0.47438995945146667	1.6264106909526639
6	0.2760642244112646	1.1357587917531917
7	0.20973625264831702	1.0066911988921607
8	0.12008274072236981	0.6587115917397152
9	0.10673086768531972	0.6586539142308122
>10	0.6328305177486179	8.279487086979845
>50	0.07653889458978289	3.804294792534742
>100	0.043994797677591735	5.319235560450488
>500	6.026684613368731E-4	0.29753337504073585
>1k	6.026684613368731E-4	0.46613562089715277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1128	0.46597128988949704	No Hit
TATCAACGCAGAGTACTTTTTTTTT	720	0.29742848290818963	No Hit
GGTATCAACGCAGAGTACTTTTTTT	440	0.1817618506661159	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	429	0.17721780439946297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	320	0.13219043684808426	No Hit
ACCATACTCCCCCCGGAACCCAAAG	318	0.13136424661778376	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	293	0.12103686873902715	No Hit
TCGTAGTTCCGACCATAAACGATGC	285	0.11773210781782506	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	280	0.11566663224207374	No Hit
GAATAGGACCGCGGTTCTATTTTGT	266	0.10988330062997005	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	248	0.10244758855726531	No Hit
GTATCTGATCGTCTTCGAACCTCCG	246	0.10162139832696478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.1309511515026334E-4	0.0
8	0.0	0.0	0.0	4.1309511515026334E-4	0.0
9	0.0	0.0	0.0	8.261902303005267E-4	0.0
10	0.0	0.0	0.0	8.261902303005267E-4	0.0
11	0.0	0.0	0.0	8.261902303005267E-4	0.0
12	0.0	0.0	0.0	8.261902303005267E-4	4.1309511515026334E-4
13	0.0	0.0	0.0	8.261902303005267E-4	4.1309511515026334E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGACC	40	2.7488108E-4	16.625181	3
CCGACCC	35	0.0021629238	16.28589	4
GGTATCA	105	0.0	16.282524	1
TCTTGCG	40	0.0052616834	14.250155	2
CCAAGAC	40	0.0052616834	14.250155	3
CGGTCCA	40	0.0052616834	14.250155	10
CGCCGGT	40	0.0052616834	14.250155	7
GCCGGTC	50	0.0014930484	13.300144	8
GGTATCT	50	0.0014930484	13.300144	11
CAAGACG	50	0.0014930484	13.300144	4
ACGGTAT	50	0.0014930484	13.300144	9
GTATCAA	340	0.0	13.129781	1
CCGTCAA	70	1.0834633E-4	12.214418	18
TATTCCA	55	0.003055333	12.091042	5
GACGGTA	55	0.003055333	12.091042	8
CAAGACC	55	0.003055333	12.091042	4
GGACAGG	55	0.003055333	12.091042	6
GTCTTGC	55	0.003060071	12.088541	1
TACGACG	65	7.979789E-4	11.692435	5
GAACTAC	65	7.993653E-4	11.690017	1
>>END_MODULE