FastQCFastQC Report
Thu 2 Feb 2017
SRR4062271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242075
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4830.1995249406175772No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG3890.16069399979345245No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG3430.14169162449654032No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3220.1330166270783848No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2850.11773210781782506No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2840.11731901270267481No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2670.11029639574512032No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT2650.10947020551481977No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC2540.1049261592481669No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG2530.10451306413301663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA301.2980617E-436.66666830
GGTCGTA353.210323E-431.4285729
CGTAAAC353.210323E-431.4285732
TACGAAT857.2759576E-1231.05882541
ATACGAA857.2759576E-1231.05882540
ATTGTCG300.005736056229.33333643
TAGCCCT300.005736056229.3333364
CGAATGC852.6193447E-1028.47058943
TTATCGG407.0157857E-427.544
GTAAACC407.0157857E-427.533
GTATCAA2100.027.2380961
CGATCGC905.0931703E-1026.8888910
GCGCAAT1006.002665E-1126.435
CGCAATA1006.002665E-1126.436
CAATACG1006.002665E-1126.438
TAACGCC1051.10958354E-1025.1428594
AATACGA1051.10958354E-1025.14285939
ATAACGC1151.2732926E-1124.8695663
GCAATAC1151.2732926E-1124.86956637
AACCGCG450.001394989224.4444457
Produced by FastQC (version 0.11.5)