##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242075
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08224723742642	32.0	32.0	32.0	21.0	32.0
2	28.65069503253124	32.0	32.0	32.0	14.0	32.0
3	30.344368480842714	32.0	32.0	32.0	21.0	32.0
4	30.93008778271197	32.0	32.0	32.0	32.0	32.0
5	30.636075596406073	32.0	32.0	32.0	32.0	32.0
6	34.23591448931116	36.0	36.0	36.0	32.0	36.0
7	34.22915625322731	36.0	36.0	36.0	32.0	36.0
8	34.071688526283175	36.0	36.0	36.0	32.0	36.0
9	34.42633481359083	36.0	36.0	36.0	32.0	36.0
10	33.944240421357016	36.0	36.0	36.0	32.0	36.0
11	34.61467313849014	36.0	36.0	36.0	32.0	36.0
12	34.25709387586492	36.0	36.0	36.0	32.0	36.0
13	34.449418568625426	36.0	36.0	36.0	32.0	36.0
14	34.302207993390475	36.0	36.0	36.0	32.0	36.0
15	34.20430032014871	36.0	36.0	36.0	32.0	36.0
16	34.25323969844057	36.0	36.0	36.0	32.0	36.0
17	34.208398223691006	36.0	36.0	36.0	32.0	36.0
18	34.23273778787566	36.0	36.0	36.0	32.0	36.0
19	34.228652277186825	36.0	36.0	36.0	32.0	36.0
20	34.25362800784881	36.0	36.0	36.0	32.0	36.0
21	34.2443870701229	36.0	36.0	36.0	32.0	36.0
22	34.20616751006919	36.0	36.0	36.0	32.0	36.0
23	34.15496437054632	36.0	36.0	36.0	32.0	36.0
24	34.08256118971393	36.0	36.0	36.0	32.0	36.0
25	34.069788288753486	36.0	36.0	36.0	32.0	36.0
26	33.985169885366105	36.0	36.0	36.0	32.0	36.0
27	33.950924300320146	36.0	36.0	36.0	32.0	36.0
28	33.88139626148921	36.0	36.0	36.0	32.0	36.0
29	33.85932872043788	36.0	36.0	36.0	32.0	36.0
30	33.826900753898585	36.0	36.0	36.0	32.0	36.0
31	33.79935144066921	36.0	36.0	36.0	32.0	36.0
32	33.79030052669627	36.0	36.0	36.0	32.0	36.0
33	33.72935660435815	36.0	36.0	36.0	32.0	36.0
34	33.69362800784881	36.0	36.0	36.0	32.0	36.0
35	33.733314055561294	36.0	36.0	36.0	32.0	36.0
36	33.62169162449654	36.0	36.0	36.0	27.0	36.0
37	33.64750593824228	36.0	36.0	36.0	27.0	36.0
38	33.568592378395124	36.0	36.0	36.0	27.0	36.0
39	33.55923164308582	36.0	36.0	36.0	27.0	36.0
40	33.524796034286894	36.0	36.0	36.0	27.0	36.0
41	33.49352886502117	36.0	36.0	36.0	21.0	36.0
42	33.4236455643912	36.0	36.0	36.0	21.0	36.0
43	33.43677372715068	36.0	36.0	36.0	21.0	36.0
44	33.30806155117216	36.0	36.0	36.0	21.0	36.0
45	33.28182588040897	36.0	36.0	36.0	21.0	36.0
46	33.25927088712176	36.0	36.0	36.0	21.0	36.0
47	33.249385521016215	36.0	36.0	36.0	21.0	36.0
48	33.18312506454611	36.0	36.0	36.0	21.0	36.0
49	33.14547970670247	36.0	36.0	36.0	21.0	36.0
50	32.52473407001962	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	4.0
21	5.0
22	23.0
23	98.0
24	221.0
25	535.0
26	1146.0
27	2206.0
28	4133.0
29	7269.0
30	11638.0
31	18688.0
32	29127.0
33	45849.0
34	67504.0
35	53628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92146413005309	18.96139307182252	13.299043605792072	25.818099192332323
2	18.528601349202088	20.751934365821313	34.936319809641056	25.783144475335547
3	21.525560260249925	22.979241970463697	28.465971289889495	27.02922647939688
4	15.867809563151916	16.800165238046063	35.303521635856654	32.02850356294537
5	20.327171331199008	32.33997727976867	30.407105235980588	16.92574615305174
6	35.52122276154084	36.10079520809666	14.576474233192194	13.801507797170299
7	30.418671899204792	30.13776722090261	19.189920479190334	20.25364040070226
8	28.796034286894557	32.229267788908395	17.540431684395333	21.434266239801715
9	26.062583909945264	13.39956625012909	17.511928121449962	43.02592171847568
10	16.311886811938447	25.430548383765363	29.90849943199422	28.349065372301972
11	38.46989569348342	20.0805535474543	20.971186615718267	20.478364143344006
12	24.46638438500465	22.856139626148924	27.510069193431786	25.167406795414642
13	29.483837653619744	18.2724362284416	24.36063203552618	27.883094082412473
14	24.053289269854382	18.22286481462357	24.192915418775172	33.530930496746876
15	25.70443044510999	25.652380460601055	21.14468656408138	27.49850253020758
16	26.281524320974903	25.88247443973975	22.362077868429207	25.473923370856138
17	24.329649901889912	26.082825570587627	23.98884643189094	25.59867809563152
18	25.508315639019475	24.490445070515626	25.203037087832648	24.798202202632254
19	25.76928637818858	25.183517504905506	24.67747598884643	24.369720128059484
20	26.286894557471857	23.77858101827946	23.948776205721366	25.985748218527316
21	26.832180109470205	23.7657750697098	24.168542806981307	25.233502013838688
22	25.91593514406692	24.01445832903026	24.623360528761747	25.44624599814107
23	24.555612929877103	23.957451203139524	25.211607972735724	26.275327894247653
24	24.660539089125272	24.796034286894557	24.85304141278529	25.690385211194876
25	24.936899721160795	24.038417845708974	24.954662811112257	26.07001962201797
26	24.98771042032428	24.574615305174017	24.90756996798513	25.530104306516577
27	25.438089179341855	24.290506208845226	24.65279212141742	25.6186124903955
28	24.620773984599623	24.468339998017118	25.465150864205693	25.44573515317757
29	25.079521138164363	24.81679155960574	24.864297694093544	25.239389608136353
30	25.001549119480487	24.52194172832162	25.57988705886241	24.896622093335484
31	25.403493987350924	24.658264242604503	24.642153400007434	25.29608837003714
32	25.87205459532701	24.71991804091345	23.909828480782576	25.498198882976965
33	24.613859341113294	24.56057007125891	25.236393679644735	25.589176907983063
34	25.36527935557162	24.349065372301972	25.137250852008673	25.148404420117732
35	26.233605287617472	24.16978209232676	24.83238665702778	24.764225963027986
36	24.30073324382939	24.81586285242177	24.97449137663947	25.90891252710937
37	25.903649297322307	24.40576022208085	24.920478861835637	24.770111618761206
38	25.136424661778374	24.137147578229886	25.078178250542187	25.64824950944955
39	25.413716466865505	24.75565323000405	24.143856837165494	25.68677346596495
40	25.77093875864918	25.0963544356088	24.587834348858824	24.544872456883198
41	24.649798616131363	24.763812867912836	25.312816275947537	25.273572240008264
42	25.790167266357937	25.57370006072649	24.289355514336822	24.346777158578753
43	25.371691542494084	24.126592004758972	25.11597932820807	25.38573712453887
44	25.186629595508496	24.89620213420806	24.36079105334694	25.556377216936504
45	25.26427978337113	25.620370714619146	24.288954158456335	24.82639534355339
46	24.72621668849222	25.179389517951346	24.64855352355301	25.44584027000343
47	24.976660029577733	24.69121012582929	25.2699587729372	25.062171071655776
48	26.162896376224865	25.494902258869406	23.761505031644	24.580696333261727
49	25.093878967393323	25.33967868400071	24.377548364708947	25.18889398389702
50	25.821136599290295	24.91769011827011	24.269538527634225	24.991634754805368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	3.5
2	2.0
3	4.5
4	7.0
5	10.0
6	13.0
7	17.0
8	21.0
9	26.0
10	31.0
11	58.0
12	85.0
13	156.0
14	227.0
15	295.0
16	363.0
17	434.0
18	505.0
19	555.5
20	606.0
21	719.0
22	832.0
23	1075.5
24	1319.0
25	1654.0
26	1989.0
27	2395.0
28	2801.0
29	3157.0
30	3513.0
31	3826.0
32	4139.0
33	4541.5
34	4944.0
35	5544.5
36	6145.0
37	7060.5
38	7976.0
39	8918.0
40	9860.0
41	10713.0
42	11566.0
43	11864.0
44	12162.0
45	12968.5
46	13775.0
47	14677.5
48	15580.0
49	15990.0
50	16400.0
51	16102.5
52	15805.0
53	16087.0
54	16369.0
55	17108.5
56	17848.0
57	17371.0
58	16894.0
59	15442.5
60	13991.0
61	12994.5
62	11998.0
63	10598.0
64	9198.0
65	7980.5
66	6763.0
67	5929.0
68	5095.0
69	4842.0
70	4589.0
71	3593.5
72	2598.0
73	2321.0
74	2044.0
75	1620.5
76	1197.0
77	1079.5
78	962.0
79	837.5
80	713.0
81	589.5
82	466.0
83	383.5
84	301.0
85	231.5
86	162.0
87	121.5
88	81.0
89	55.0
90	29.0
91	27.0
92	25.0
93	21.0
94	17.0
95	13.0
96	9.0
97	12.5
98	16.0
99	27.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008261902303005266
2	0.00247857069090158
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.130951151502634E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	4.130951151502634E-4
28	0.00123928534545079
29	4.130951151502634E-4
30	8.261902303005268E-4
31	8.261902303005268E-4
32	0.00123928534545079
33	0.0
34	0.0
35	0.0
36	0.0
37	4.130951151502634E-4
38	0.0
39	4.130951151502634E-4
40	0.0
41	0.0
42	0.00247857069090158
43	0.00247857069090158
44	0.0074357120727047395
45	8.261902303005268E-4
46	0.0016523804606010536
47	4.130951151502634E-4
48	0.002891665806051843
49	0.003304760921202107
50	8.261902303005268E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	242075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.99997671845559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.42515827284882	66.17459630235018
2	6.672554644653632	9.87537776713983
3	1.6618553450983442	3.689317705401554
4	0.6783447060856209	2.0078996982969417
5	0.368346631768792	1.3628821087606071
6	0.21379066062108626	0.9492302345150175
7	0.16641206437925962	0.862014222281474
8	0.11828988083924619	0.7002758742506483
9	0.08677934925421242	0.5779502842009
>10	0.5279444218201201	7.331873628846475
>50	0.050886708745888985	2.574273906357646
>100	0.029637313884968312	3.8943082675987473
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	483	0.1995249406175772	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	389	0.16069399979345245	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	343	0.14169162449654032	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	322	0.1330166270783848	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	285	0.11773210781782506	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	284	0.11731901270267481	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	267	0.11029639574512032	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	265	0.10947020551481977	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	254	0.1049261592481669	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	253	0.10451306413301663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1309511515026334E-4
2	0.0	0.0	0.0	0.0	4.1309511515026334E-4
3	0.0	0.0	0.0	0.0	4.1309511515026334E-4
4	0.0	0.0	0.0	0.0	4.1309511515026334E-4
5	0.0	0.0	0.0	0.0	4.1309511515026334E-4
6	0.0	0.0	0.0	0.0	4.1309511515026334E-4
7	0.0	0.0	0.0	0.0	4.1309511515026334E-4
8	0.0	0.0	0.0	0.0	4.1309511515026334E-4
9	0.0	0.0	0.0	0.0	4.1309511515026334E-4
10	0.0	0.0	0.0	0.0	8.261902303005267E-4
11	0.0	0.0	0.0	0.0	8.261902303005267E-4
12	0.0	0.0	0.0	0.0	0.00123928534545079
13	0.0	0.0	0.0	0.0	0.0016523804606010533
14	0.0	0.0	0.0	0.0	0.0016523804606010533
15	0.0	0.0	0.0	0.0	0.0016523804606010533
16	0.0	0.0	0.0	0.0	0.0016523804606010533
17	0.0	0.0	0.0	0.0	0.0016523804606010533
18	0.0	0.0	0.0	0.0	0.0016523804606010533
19	0.0	0.0	0.0	0.0	0.0016523804606010533
20	0.0	0.0	0.0	0.0	0.00247857069090158
21	0.0	0.0	0.0	0.0	0.0028916658060518436
22	0.0	0.0	0.0	0.0	0.0028916658060518436
23	0.0	0.0	0.0	0.0	0.0028916658060518436
24	0.0	0.0	0.0	0.0	0.0028916658060518436
25	0.0	0.0	0.0	0.0	0.0028916658060518436
26	0.0	0.0	0.0	4.1309511515026334E-4	0.0033047609212021067
27	0.0	0.0	0.0	4.1309511515026334E-4	0.00371785603635237
28	0.0	0.0	0.0	8.261902303005267E-4	0.00371785603635237
29	0.0	0.0	0.0	0.00123928534545079	0.00371785603635237
30	0.0	0.0	0.0	0.0020654755757513166	0.00371785603635237
31	0.0	0.0	0.0	0.0033047609212021067	0.00371785603635237
32	0.0	0.0	0.0	0.004130951151502633	0.00371785603635237
33	0.0	0.0	0.0	0.004544046266652897	0.00371785603635237
34	0.0	0.0	0.0	0.005370236496953423	0.00371785603635237
35	0.0	0.0	0.0	0.00619642672725395	0.00371785603635237
36	0.0	0.0	0.0	0.008261902303005266	0.00371785603635237
37	0.0	0.0	0.0	0.010740472993906847	0.00371785603635237
38	0.0	0.0	0.0	0.014045233915108955	0.00371785603635237
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAA	30	1.2980617E-4	36.666668	30
GGTCGTA	35	3.210323E-4	31.42857	29
CGTAAAC	35	3.210323E-4	31.42857	32
TACGAAT	85	7.2759576E-12	31.058825	41
ATACGAA	85	7.2759576E-12	31.058825	40
ATTGTCG	30	0.0057360562	29.333336	43
TAGCCCT	30	0.0057360562	29.333336	4
CGAATGC	85	2.6193447E-10	28.470589	43
TTATCGG	40	7.0157857E-4	27.5	44
GTAAACC	40	7.0157857E-4	27.5	33
GTATCAA	210	0.0	27.238096	1
CGATCGC	90	5.0931703E-10	26.88889	10
GCGCAAT	100	6.002665E-11	26.4	35
CGCAATA	100	6.002665E-11	26.4	36
CAATACG	100	6.002665E-11	26.4	38
TAACGCC	105	1.10958354E-10	25.142859	4
AATACGA	105	1.10958354E-10	25.142859	39
ATAACGC	115	1.2732926E-11	24.869566	3
GCAATAC	115	1.2732926E-11	24.869566	37
AACCGCG	45	0.0013949892	24.444445	7
>>END_MODULE