FastQCFastQC Report
Thu 2 Feb 2017
SRR4062270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140292
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21680.19012673946673309No Hit
TATCAACGCAGAGTACTTTTTTTTT14780.12961592293903665No Hit
GCGCAAGACGGACCAGAGCGAAAGC13530.11865381849561339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCA453.5346704E-516.890494
AACCGCG508.697115E-515.2027777
GGTATCA6400.014.3909521
AGGGCGA604.088776E-412.66731210
GTTCAAA2950.012.2309261
ACTATAC701.08988796E-412.2149083
GTATCAA16450.011.9481931
CAAGACG2650.011.8313114
AAGACGG2500.011.7816345
GGCGACA658.017108E-411.69290312
GGGTAGG3050.011.5185991
TCGCGTA752.0702176E-411.4015819
CGGTCCA1501.7644197E-1011.40057910
GTCTTAC752.0829237E-411.3940731
CGTTATT1354.7220965E-911.2588432
CTAAGGT600.005869877511.0843844
TCGTCAC1803.6379788E-1211.0819519
GTGTTAC600.00589680311.0775711
CTTGGAC2000.010.9255553
CGTCGTA2000.010.92555510
Produced by FastQC (version 0.11.5)