##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062270_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140292
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217957330227698	32.0	32.0	32.0	32.0	32.0
2	30.751330360995254	32.0	32.0	32.0	32.0	32.0
3	30.78191550936076	32.0	32.0	32.0	32.0	32.0
4	30.7758740743599	32.0	32.0	32.0	32.0	32.0
5	30.70339965552683	32.0	32.0	32.0	32.0	32.0
6	34.35015241710018	36.0	36.0	36.0	32.0	36.0
7	34.27110073560106	36.0	36.0	36.0	32.0	36.0
8	34.225669389945736	36.0	36.0	36.0	32.0	36.0
9	34.36657891136656	36.0	36.0	36.0	32.0	36.0
10	34.04781845351892	36.0	36.0	36.0	32.0	36.0
11	34.3458859660508	36.0	36.0	36.0	32.0	36.0
12	34.166825690261796	36.0	36.0	36.0	32.0	36.0
13	34.26236788471725	36.0	36.0	36.0	32.0	36.0
14	34.163549336485744	36.0	36.0	36.0	32.0	36.0
15	34.11725242306357	36.0	36.0	36.0	32.0	36.0
16	34.12348416019756	36.0	36.0	36.0	32.0	36.0
17	34.051290371238245	36.0	36.0	36.0	32.0	36.0
18	34.062906694074854	36.0	36.0	36.0	32.0	36.0
19	34.064094109228165	36.0	36.0	36.0	32.0	36.0
20	34.04839374476011	36.0	36.0	36.0	32.0	36.0
21	34.030858762492414	36.0	36.0	36.0	32.0	36.0
22	34.00076208550091	36.0	36.0	36.0	32.0	36.0
23	33.967087377619066	36.0	36.0	36.0	32.0	36.0
24	33.940790604511825	36.0	36.0	36.0	32.0	36.0
25	33.5980406773002	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	18.0
5	93.0
6	219.0
7	52.0
8	205.0
9	138.0
10	87.0
11	27.0
12	41.0
13	48.0
14	193.0
15	250.0
16	412.0
17	548.0
18	760.0
19	1097.0
20	1636.0
21	2225.0
22	3394.0
23	5059.0
24	7203.0
25	10120.0
26	13809.0
27	17711.0
28	23762.0
29	30794.0
30	40344.0
31	55287.0
32	77528.0
33	108845.0
34	237294.0
35	501093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77709061952987	17.71541124842583	12.021303464750886	25.48619466729341
2	16.747903168662063	19.38064986863014	37.36367526914159	26.507771693566205
3	19.32611643043938	22.644669845986005	28.14127332967343	29.88794039390118
4	12.991163637160962	15.547174775801961	35.03181529151227	36.42984629552481
5	15.248277967457707	36.13610285525803	33.00881105188731	15.606808125396954
6	34.76635579626464	34.60191155462111	16.56192194426797	14.069810704846278
7	30.8600660002265	30.167406001473108	19.776013596854323	19.19651440144607
8	28.253472459981776	32.82187961436425	19.00069001480108	19.923957910852895
9	26.828486664600455	14.765562648764972	18.895993804880064	39.50995688175451
10	16.78611821400472	26.540371754523996	30.346869028886143	26.326641002585138
11	36.911171549182725	21.461496781921504	21.790900859998434	19.836430808897333
12	24.38795652998574	23.74131548074829	28.19931015588229	23.67141783338368
13	29.11342722674909	19.654221691486374	25.109142315257095	26.123208766507446
14	23.589546387969232	19.71039838878046	24.83904924702352	31.861005976226785
15	25.27533320103056	27.09979434528566	21.96808576029898	25.656786693384802
16	25.78567483970434	26.05626568825511	23.474151199001923	24.683908273038632
17	23.975759427596568	26.017787073158093	25.012007020704758	24.994446478540578
18	24.631604453141666	25.273185544935693	25.87341678803857	24.22179321388407
19	25.415295807188983	25.098227860546285	25.347440457589087	24.139035874675645
20	25.76650498305043	24.404219573724042	24.860304302258832	24.968971140966694
21	26.50963559839019	24.224390286719945	24.602528823408072	24.66344529148179
22	25.84050396001187	24.261151853418987	25.043058874341828	24.855285312227316
23	24.40736934215342	24.39420416037298	25.418016463498656	25.780410033974942
24	24.491470824197332	24.970638990367483	25.44401395284332	25.09387623259186
25	24.747998736043815	24.507847061301874	25.31686679306229	25.427287409592026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	586.0
1	586.0
2	500.5
3	415.0
4	415.0
5	415.0
6	843.5
7	1272.0
8	1272.0
9	1272.0
10	1519.0
11	1766.0
12	1766.0
13	1766.0
14	2414.5
15	3063.0
16	3063.0
17	3063.0
18	5101.0
19	7139.0
20	7139.0
21	7139.0
22	11315.5
23	15492.0
24	15492.0
25	15492.0
26	22731.5
27	29971.0
28	29971.0
29	29971.0
30	37755.0
31	45539.0
32	45539.0
33	45539.0
34	56852.5
35	68166.0
36	68166.0
37	68166.0
38	80405.0
39	92644.0
40	92644.0
41	92644.0
42	106337.0
43	120030.0
44	120030.0
45	120030.0
46	133519.5
47	147009.0
48	147009.0
49	147009.0
50	152378.5
51	157748.0
52	157748.0
53	157748.0
54	147290.0
55	136832.0
56	136832.0
57	136832.0
58	125439.5
59	114047.0
60	114047.0
61	114047.0
62	100016.0
63	85985.0
64	85985.0
65	85985.0
66	70285.5
67	54586.0
68	54586.0
69	54586.0
70	41560.5
71	28535.0
72	28535.0
73	28535.0
74	21655.0
75	14775.0
76	14775.0
77	14775.0
78	11704.5
79	8634.0
80	8634.0
81	8634.0
82	5998.5
83	3363.0
84	3363.0
85	3363.0
86	2480.5
87	1598.0
88	1598.0
89	1598.0
90	1063.0
91	528.0
92	528.0
93	528.0
94	317.0
95	106.0
96	106.0
97	106.0
98	284.5
99	463.0
100	463.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.015746843790888E-4
2	0.0
3	5.261810132843166E-4
4	0.010260529759044176
5	0.031570860797059
6	0.061036997540980735
7	0.10567468683460027
8	0.10400844695919993
9	0.11672448811357092
10	0.1294405292679419
11	0.1375963349738488
12	0.13014210395232098
13	0.12382793179290918
14	0.11216425266510684
15	0.13066828496560529
16	0.1142689767182441
17	0.12058314887765591
18	0.10795480455883229
19	0.09681730644431427
20	0.09032774061380769
21	0.09006465010716554
22	0.10120214822168358
23	0.08094417921023737
24	0.08997695327161816
25	0.08874919757395475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1140292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37569298294399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45649662667803	42.11750680106682
2	13.380373994929437	14.551342166905018
3	3.9332295454482593	6.4161624658421665
4	1.6576173361768902	3.605363654206402
5	0.8479712285732836	2.3054511591635345
6	0.5268646938346307	1.7189179701302804
7	0.3967093948597477	1.50999437808402
8	0.25797471612180606	1.1222043168961167
9	0.20332540669960467	0.9950363901296934
>10	1.1788110857877296	11.629777141350468
>50	0.08300817900952691	3.136481655233304
>100	0.06983981149404332	7.549973842611668
>500	0.0061575678062453214	2.2609297223097538
>1k	0.0016204125805908737	1.080858336070684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2168	0.19012673946673309	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1478	0.12961592293903665	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1353	0.11865381849561339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.76968355473861E-5	0.0
10	0.0	0.0	0.0	8.76968355473861E-5	0.0
11	0.0	0.0	0.0	8.76968355473861E-5	0.0
12	0.0	0.0	0.0	8.76968355473861E-5	0.0
13	0.0	0.0	0.0	8.76968355473861E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCA	45	3.5346704E-5	16.89049	4
AACCGCG	50	8.697115E-5	15.202777	7
GGTATCA	640	0.0	14.390952	1
AGGGCGA	60	4.088776E-4	12.667312	10
GTTCAAA	295	0.0	12.230926	1
ACTATAC	70	1.08988796E-4	12.214908	3
GTATCAA	1645	0.0	11.948193	1
CAAGACG	265	0.0	11.831311	4
AAGACGG	250	0.0	11.781634	5
GGCGACA	65	8.017108E-4	11.692903	12
GGGTAGG	305	0.0	11.518599	1
TCGCGTA	75	2.0702176E-4	11.401581	9
CGGTCCA	150	1.7644197E-10	11.400579	10
GTCTTAC	75	2.0829237E-4	11.394073	1
CGTTATT	135	4.7220965E-9	11.258843	2
CTAAGGT	60	0.0058698775	11.084384	4
TCGTCAC	180	3.6379788E-12	11.08195	19
GTGTTAC	60	0.005896803	11.077571	1
CTTGGAC	200	0.0	10.925555	3
CGTCGTA	200	0.0	10.925555	10
>>END_MODULE