FastQCFastQC Report
Thu 2 Feb 2017
SRR4062270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140292
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12580.11032261911861171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.028.7372591
GTATCAA16850.024.5526981
CGTCTTA852.353172E-723.29332715
CGCAATA2200.019.0026936
CGTCGTA2550.018.1162410
ATACGAA2400.017.41989540
GTAGCAC1551.4400939E-817.0309353
ACCGTCG2600.016.9217628
CGGACCA3650.016.87549
TCAACGC24900.016.5208054
TCGTGAG800.001989290116.49871813
ATCAACG24950.016.4876963
CAACGCA25600.016.0690635
TATCAAC25850.015.9994572
ACGGACC3850.015.9987558
ATACCGT2900.015.9297966
AACGCAG26400.015.9154286
ATTTGCG1259.442281E-615.83946316
CTATTCC3200.015.8112714
GCGCAAA2101.5097612E-1015.71306322
Produced by FastQC (version 0.11.5)