FastQCFastQC Report
Thu 2 Feb 2017
SRR4062269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341671
Sequences flagged as poor quality0
Sequence length25
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5140.1504371164073041No Hit
TATCAACGCAGAGTACTTTTTTTTT3580.10477915889847252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT402.752394E-416.625644
AGGCGCG350.00216497916.2863418
GTATATG551.1274478E-515.5392251
CTAACAC508.690235E-515.19835953
TTAGCTT400.00526659414.2505494
TGGACAG602.5557658E-514.2505495
GGTATCA1301.8189894E-1213.8790511
TAACACT500.001494935813.3005134
TAAAGTG604.076179E-412.6671555
AGGGACG604.076179E-412.6671558
GTACAAG604.0913236E-412.6615921
GGACAGT701.0857076E-412.2147566
GGACCCA550.003059135312.0913746
GCACTGT550.003059135312.0913746
GTATCAA3000.011.7119711
CCAAGAC658.00256E-411.6910463
CCAGACT600.0058580711.083764
TTCTATG600.00586446411.0821372
GTCCTGA600.005877269411.0788931
TAGAGCA700.001485765310.8575614
Produced by FastQC (version 0.11.5)