##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062269_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341671
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.039854128679345	32.0	14.0	32.0	14.0	32.0
2	23.751316324768563	32.0	14.0	32.0	14.0	32.0
3	24.309121933087678	32.0	14.0	32.0	14.0	32.0
4	24.204155459491734	27.0	14.0	32.0	14.0	32.0
5	24.26805903925121	21.0	14.0	32.0	14.0	32.0
6	27.946348973134974	32.0	21.0	36.0	14.0	36.0
7	27.254118728250276	32.0	21.0	36.0	14.0	36.0
8	26.65815360390551	32.0	21.0	36.0	14.0	36.0
9	27.483880692244878	32.0	21.0	36.0	14.0	36.0
10	25.265190782946167	21.0	14.0	36.0	14.0	36.0
11	28.108057166104235	32.0	21.0	36.0	14.0	36.0
12	26.422014159820414	32.0	14.0	36.0	14.0	36.0
13	27.11275466750178	32.0	21.0	36.0	14.0	36.0
14	26.254505064813813	32.0	14.0	36.0	14.0	36.0
15	26.602766989296722	32.0	14.0	36.0	14.0	36.0
16	26.779554015412486	32.0	21.0	36.0	14.0	36.0
17	26.203757415759604	32.0	14.0	36.0	14.0	36.0
18	26.53972095963661	32.0	14.0	36.0	14.0	36.0
19	26.558698279924254	32.0	14.0	36.0	14.0	36.0
20	26.255640660167238	32.0	14.0	36.0	14.0	36.0
21	26.14581278481346	32.0	14.0	36.0	14.0	36.0
22	26.294259682560124	32.0	14.0	36.0	14.0	36.0
23	25.86089834958191	32.0	14.0	36.0	14.0	36.0
24	25.722724492274732	32.0	14.0	36.0	14.0	36.0
25	25.18292158245798	27.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
3	9.0
4	30.0
5	46.0
6	58.0
7	24.0
8	35.0
9	32.0
10	10.0
11	16.0
12	21.0
13	18.0
14	599.0
15	1561.0
16	3368.0
17	6519.0
18	11012.0
19	16077.0
20	20249.0
21	22486.0
22	23141.0
23	22904.0
24	22118.0
25	21577.0
26	20930.0
27	20083.0
28	19377.0
29	18639.0
30	17692.0
31	16976.0
32	16687.0
33	14824.0
34	15000.0
35	9553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.886954587494294	18.279148178933934	11.838685507568751	26.99521172600302
2	16.11379401176574	19.92478122164662	38.10255509702344	25.8588696695642
3	18.48035098091439	23.283802254215313	29.01972950586968	29.21611725900062
4	12.528795183191516	15.82059320272687	36.33330113541567	35.31731047866595
5	14.177267071653372	36.65607846007465	34.30022689014125	14.866427578130716
6	33.59796416795029	35.63643178849837	17.10280483305123	13.662799210500115
7	29.286641258272134	30.647972650333816	21.208002179536383	18.857383911857674
8	26.86231372468604	34.09449764620067	19.75550953090991	19.287679098203377
9	26.576296808442073	14.522848320433118	18.870152165888356	40.03070270523645
10	14.998813315479348	27.5102772739659	32.7952720144629	24.69563739609185
11	35.74143261102076	21.57443628851479	23.05481077754824	19.629320322916207
12	23.156802878557908	23.973429597810608	29.83925316893361	23.030514354697875
13	28.691807572743283	20.305417766617932	25.463298007928437	25.53947665271035
14	22.174533373567748	19.679609062118224	26.341431974054665	31.804425590259367
15	24.432222733359314	27.43292302373076	22.639010568817447	25.495843674092477
16	24.698158038913515	26.441092109773496	24.324316405963884	24.536433445349097
17	23.035589242655526	26.494018651610997	26.044282194245135	24.42610991148835
18	23.55503733802854	26.249117451463494	26.354583832589697	23.84126137791827
19	24.47412128970271	25.85293264710104	25.822468151420257	23.85047791177599
20	24.48226368669264	25.321772752570375	25.94217756817716	24.253785992559827
21	24.819269344331442	25.150267141585044	25.518172657824433	24.51229085625908
22	24.421809958197482	25.324650595986135	25.765525080045816	24.488014365770564
23	23.4265027634504	25.39211120581335	25.868063182991087	25.313322847745155
24	23.407184365919957	25.617150154653668	25.9952900927922	24.980375386634172
25	23.779089351356895	25.14579458414493	26.053514154742903	25.021601909755276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	55.0
2	87.5
3	120.0
4	120.0
5	120.0
6	251.5
7	383.0
8	383.0
9	383.0
10	518.5
11	654.0
12	654.0
13	654.0
14	978.5
15	1303.0
16	1303.0
17	1303.0
18	2232.5
19	3162.0
20	3162.0
21	3162.0
22	4590.5
23	6019.0
24	6019.0
25	6019.0
26	8609.5
27	11200.0
28	11200.0
29	11200.0
30	13961.5
31	16723.0
32	16723.0
33	16723.0
34	20113.5
35	23504.0
36	23504.0
37	23504.0
38	27146.0
39	30788.0
40	30788.0
41	30788.0
42	34299.5
43	37811.0
44	37811.0
45	37811.0
46	40964.5
47	44118.0
48	44118.0
49	44118.0
50	44618.5
51	45119.0
52	45119.0
53	45119.0
54	42183.5
55	39248.0
56	39248.0
57	39248.0
58	35413.5
59	31579.0
60	31579.0
61	31579.0
62	27194.5
63	22810.0
64	22810.0
65	22810.0
66	18300.5
67	13791.0
68	13791.0
69	13791.0
70	10302.5
71	6814.0
72	6814.0
73	6814.0
74	5110.0
75	3406.0
76	3406.0
77	3406.0
78	2627.5
79	1849.0
80	1849.0
81	1849.0
82	1265.5
83	682.0
84	682.0
85	682.0
86	479.5
87	277.0
88	277.0
89	277.0
90	183.0
91	89.0
92	89.0
93	89.0
94	55.5
95	22.0
96	22.0
97	22.0
98	83.5
99	145.0
100	145.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.780376444006077E-4
2	2.9267921480020253E-4
3	0.0
4	0.009951093303206886
5	0.028097204620819445
6	0.05531637159723828
7	0.0919012734472636
8	0.09014519815846239
9	0.09746217852846745
10	0.11238881848327778
11	0.11882776120888223
12	0.11385221455727879
13	0.10770595104647454
14	0.09892557460246847
15	0.11238881848327778
16	0.1024377251800709
17	0.10536451732807292
18	0.09658414088406685
19	0.08487697229205873
20	0.0822428593588569
21	0.0816575009292565
22	0.08897448129926157
23	0.07316980370005063
24	0.07697463349245326
25	0.07785267113685387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	341671.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.5339631513767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14183486327357	64.31100217203104
2	10.190477891034746	15.394543630326746
3	2.3449289359611747	5.31365327524465
4	0.8497732938211375	2.5674697865003924
5	0.4220870275956324	1.5940952994541302
6	0.24594434136297694	1.1146290490680426
7	0.17982409579689387	0.9507978637956551
8	0.11737459201027287	0.7092614486249459
9	0.07544843519165728	0.5129027391236105
>10	0.4028099769071863	5.436746790722838
>50	0.020958072900712346	1.0653315327121415
>100	0.00815036168293735	0.8788838565952802
>500	3.8811246109225485E-4	0.15068255580052503
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	514	0.1504371164073041	No Hit
TATCAACGCAGAGTACTTTTTTTTT	358	0.10477915889847252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACT	40	2.752394E-4	16.62564	4
AGGCGCG	35	0.002164979	16.28634	18
GTATATG	55	1.1274478E-5	15.539225	1
CTAACAC	50	8.690235E-5	15.1983595	3
TTAGCTT	40	0.005266594	14.250549	4
TGGACAG	60	2.5557658E-5	14.250549	5
GGTATCA	130	1.8189894E-12	13.879051	1
TAACACT	50	0.0014949358	13.300513	4
TAAAGTG	60	4.076179E-4	12.667155	5
AGGGACG	60	4.076179E-4	12.667155	8
GTACAAG	60	4.0913236E-4	12.661592	1
GGACAGT	70	1.0857076E-4	12.214756	6
GGACCCA	55	0.0030591353	12.091374	6
GCACTGT	55	0.0030591353	12.091374	6
GTATCAA	300	0.0	11.711971	1
CCAAGAC	65	8.00256E-4	11.691046	3
CCAGACT	60	0.00585807	11.08376	4
TTCTATG	60	0.005864464	11.082137	2
GTCCTGA	60	0.0058772694	11.078893	1
TAGAGCA	70	0.0014857653	10.857561	4
>>END_MODULE